miR169a调节马铃薯气孔特性适应动态环境

doi:10.16420/j.issn.0513-353x.2024-0962

摘要/Abstract

摘要：

为了验证马铃薯MicroRNA169a（miR169a）作用于气孔的特定功能，分别构建了马铃薯miR169a过表达（OE169a）与沉默株系（STTM169），以野生型为对照，测定相关指标。结果显示OE169a提高马铃薯处于动态环境下的气孔开度，降低其净光合速率（P_n）、胞间二氧化碳浓度（C_i）、内部水分利用率（iWUE）及抗氧化能力；STTM169降低马铃薯处于动态环境下的气孔开度，提高其P_n、C_i、iWUE及抗氧化能力。综上所述，沉默miR169可降低马铃薯处于动态环境下的气孔开度，并在逆境下维持较高的光能及水份利用率，从而提升马铃薯对动态环境的适应性。

关键词: miR169, 气孔, 动态环境, 光合特性, 马铃薯

Abstract:

To verify the specific function of potato microRNA169a（miR169a）in stomata，potato miR169a overexpression（OE169a）and silenced（STTM169）lines were constructed，using the wild type as a control，and measured relevant parameters. The results showed that OE169a increased the stomatal aperture of potatoes under dynamic environmental conditions，while reducing their net photosynthetic rate （P_n），intercellular carbon dioxide concentration（C_i），intrinsic water use efficiency（iWUE），and antioxidant capacity. In contrast，STTM169 decreased the stomatal aperture under dynamic environmental conditions and enhanced P_n，C_i，iWUE and antioxidant capacity. In conclusion, silencing miR169 reduces the stomatal aperture of potatoes under dynamic environmental conditions and helps maintain higher light and water use efficiency under stress，thereby improving potato adaptability to dynamic environments.

Key words: miR169, stomata, dynamic environment, photosynthetic characteristics, potato

张宏冠, 雷紫芊, 王明, 张幸媛, 毛俊, 林原, 胡新喜, 熊兴耀, 秦玉芝. miR169a调节马铃薯气孔特性适应动态环境[J]. 园艺学报, 2025, 52(11): 2889-2898.

ZHANG Hongguan, LEI Ziqian, WANG Ming, ZHANG Xingyuan, MAO Jun, LIN Yuan, HU Xinxi, XIONG Xingyao, QIN Yuzhi. MiR169a Regulates the Stomatal Characteristics of Potatoes to Adapt to the Dynamic Environment[J]. Acta Horticulturae Sinica, 2025, 52(11): 2889-2898.

图/表 10

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名称 Name	序列 Sequence
StActin-F	ATTCAAGTATGCCTGGGTGCT
StActin-R	GTGGTGGAGTCAATAATGAGGAC
St169a-F	TCTTCGTCAACATGGTGGAGCACGA
St169a -R	GCGATTAAGTTGGGTAACGCCAGGG

名称 Name	序列 Sequence
StActin-F	ATTCAAGTATGCCTGGGTGCT
StActin-R	GTGGTGGAGTCAATAATGAGGAC
St169a-F	TCTTCGTCAACATGGTGGAGCACGA
St169a -R	GCGATTAAGTTGGGTAACGCCAGGG

株系 Lines	气孔密度/（个 · mm^-2） Stomatal density	气孔长度/μm Stomatal length	气孔宽度/μm Stomatal width
WT	156.15 ± 2.52 b	26.17 ± 0.76 a	33.50 ± 2.50 a
OE169a	261.46 ± 2.02 a	24.25 ± 1.75 a	35.75 ± 2.25 a
STTM169	86.62 ± 3.06 c	25.50 ± 0.50 a	31.50 ± 5.10 a

株系 Lines	气孔密度/（个 · mm^-2） Stomatal density	气孔长度/μm Stomatal length	气孔宽度/μm Stomatal width
WT	156.15 ± 2.52 b	26.17 ± 0.76 a	33.50 ± 2.50 a
OE169a	261.46 ± 2.02 a	24.25 ± 1.75 a	35.75 ± 2.25 a
STTM169	86.62 ± 3.06 c	25.50 ± 0.50 a	31.50 ± 5.10 a

株系 Lines	净光合速率/ （μmol · m^-2· s^-1） Net photosynthetic rate	气孔导度/ （mmol · m^-2· s^-1） Stomatal conductance	胞间二氧化碳浓度/ （μmol · mol^-1） Intercellular CO₂concentration	蒸腾速率/ （mmol · m^-2· s^-1） Transpiration rate	内部水分利用率/% Intrinsic water use effficiency	SPAD值 SPAD value
WT	16.29 ± 0.23 b	0.14 ± 0 b	263.20 ± 7.06 b	4.55 ± 0.42 b	115.03 ± 4.77 b	30.23 ± 0.55 a
OE169a	18.15 ± 1.21 a	0.19 ± 0.01 a	278.77 ± 6.26 a	5.72 ± 0.38 a	93.83 ± 7.92 b	29.37 ± 0.64 a
STTM169	13.63 ± 0.52 c	0.07 ± 0 c	199.21 ± 6.61 c	2.53 ± 0.32 c	196.50 ± 26.04 a	29.77 ± 0.58 a