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园艺学报 ›› 2024, Vol. 51 ›› Issue (6): 1321-1331.doi: 10.16420/j.issn.0513-353x.2023-0788

• 遗传育种·种质资源·分子生物学 • 上一篇    下一篇

蟹爪兰品种的DUS测试性状主成分分析及其亲缘关系研究

赵天荣*(), 凌建刚, 任锡亮   

  1. 宁波市农业科学研究院,浙江宁波 315040
  • 收稿日期:2024-02-24 修回日期:2024-05-20 出版日期:2024-12-18 发布日期:2024-06-22
  • 通讯作者:
  • 基金资助:
    宁波市科技创新2025重大专项(2019B10013)

Principal Component Analysis and Genetic Relationship Research on Cultivars of Schlumbergera truncata Based on Morphological Characteristics for DUS Testing

ZHAO Tianrong*(), LING Jiangang, REN Xiliang   

  1. Ningbo Academy of Agricultural Sciences,Ningbo,Zhejiang 315040,China
  • Received:2024-02-24 Revised:2024-05-20 Published:2024-12-18 Online:2024-06-22

摘要:

对58个蟹爪兰品种DUS(特异性、一致性和稳定性)测试中的16个性状的原始数据平均值进行统计分析,揭示各性状变异情况,并进行主成分分析和聚类分析。分析结果显示花托颜色变异系数最大,为62.74%,内轮花被片宽度变异系数最小,为10.35%。按照方差累计贡献率86.8%提取10个主成分,通过各性状在各主成分的得分,筛选出蟹爪兰资源评价中性状叶状茎宽度、叶状茎长度、始花期、叶状茎边缘缺刻的类型、叶状茎边缘缺刻的深度、花长度、内轮花被片宽度、内轮花被片边缘区的颜色、花宽度、花托颜色和雌蕊花筒以上部分长度具有重要的地位。聚类分析结果显示,58个供试品种依据16个性状聚为3大类,并细分为6小类。其中C类10个品种中有9个的叶状茎边缘缺刻类型为钝锯齿或密钝锯齿,说明聚入C类的品种具有仙人指(Schlumbergera bridgesii )的基因,与A类和B类距离较远。结果表明DUS测试的相关性状在蟹爪兰品种间具有广泛的多态性,类间距离的远近能够较准确地反映品种间的亲缘关系。

关键词: 蟹爪兰, DUS测试, 表型, 亲缘关系

Abstract:

Sixteen morphological characteristics,often used for DUS(Distinctness,Uniformity and Stability)testing,of 58 cultivars of Schlumbergera truncata were recorded for statistical analysis in order to reveal the variation of each characteristic and the principal component analysis and cluster analysis were performed by using the DPS software. The results showed that the maximum coefficient of variation of receptacle color was 62.74%,and the minimum coefficient of variation of inner perianth width was 10.35%. According to the cumulative contribution rate of variance 86.8%,10 principal components were extracted,and the characters leafy stem width,leafy stem length,the beginning of flowering,leafy stem type of margin notch,leafy stem depth of margin notch,flower length,inner perianth segments width,inner perianth segments color of marginal zone,flower width,receptacle color and pistil length above tube were screened out by the scores of each trait in principal component. Based on the results of cluster analysis,58 cultivars were divided into three groups according to the differences of 16 characteristics,and were further subdivided into six groups. Nine of the 10 cultivars clustered into class C had type of margin notch with obtuse serrated or dense serrated type,which indicated that the cultivars clustered into class C had genes of Schlumbergera bridgesii,so it is far away from class A and class B. The results showed that there was a wide range of polymorphisms among S. truncata cultivars in the DUS-test. The distance between classes could accurately reflect the relationship between cultivars.

Key words: Schlumbergera truncata, DUS testing, morphological characteristics, genetic relationship