李资源果实主要性状的SSR标记关联分析及指纹图谱构建

doi:10.16420/j.issn.0513-353x.2023-0988

摘要/Abstract

摘要：

2022和2023年对收集的102份李资源的11个果实性状进行鉴定，其中单果质量的变异系数最大，可食率和果形指数的变异系数较小。利用78对多态性引物在供试材料中共扩增出405个等位变异，多态性信息含量（PIC）为0.06 ~ 0.80，平均值0.53。对SSR标记和11个主要性状进行关联分析，共检测到55个SSR标记与主要性状极显著关联，表型变异解释率为4.01% ~ 14.76%。其中有29个标记在两年的GLM和MLM模型下，出现2次及以上与同一性状极显著关联。筛选出可区分102份供试材料的27对核心引物，并构建了102个李种质的指纹图谱。

关键词: 李, SSR, 关联分析, 指纹图谱

Abstract:

A study on eleven fruit features generated from 102 plum resources was carried out in 2022 and 2023. The coefficient of variation for fruit weight was the highest，while edible ratio and fruit shape indices had lower coefficients. A total of 405 allelic variants were amplified in the test material using 78 pairs of polymorphic primers，with polymorphism information content（PIC）ranging from 0.06 to 0.80 and a mean value of 0.53. An association analysis revealed that 55 SSR markers had highly significant associations with 11 major traits，explaining 4.01%-14.76% of the phenotypic variance. Among the test materials，29 exhibited two or more highly significant associations with traits in two years under GLM and MLM models. From 78 primers，27 pairs of primers were selected to distinguish 102 materials，and the fingerprints of 102 plum resources were constructed.

Key words: plum, SSR, association analysis, fingerprint mapping

岳林清, 吴怡超, 何星星, 向明燕, 王进, 武峥, 朱世国, 丁梦琦, 张建, 方小梅, 易泽林. 李资源果实主要性状的SSR标记关联分析及指纹图谱构建[J]. 园艺学报, 2024, 51(11): 2495-2509.

YUE Linqing, WU Yichao, HE Xingxing, XIANG Mingyan, WANG Jin, WU Zheng, ZHU Shiguo, DING Mengqi, ZHANG Jian, FANG Xiaomei, YI Zelin. SSR Marker Association Analysis and Fingerprint Construction of Main Traits of Plum Fruit[J]. Acta Horticulturae Sinica, 2024, 51(11): 2495-2509.

图/表 12

参考文献 26

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编号 No.	引物名 Primer name	正向引物序列（5′-3′） Forward primer sequence	反向引物序列（5′-3′） Reverse primer sequence
1	BPPCT001	AATTCCCAAAGGATGTGTATGAG	CAGGTGAATGAGCCAAAGC
2	BPPCT002	TCGACAGCTTGATCTTGACC	CAATGCCTACGGAGATAAAAGAC
3	BPPCT004	CTGAGTGATCCATTTGCAGG	AGGGCATCTAGACCTCATTGTT
4	BPPCT007	TCATTGCTCGTCATCAGC	CAGATTTCTGAAGTTAGCGGTA
5	BPPCT008	ATGGTGTGTATGGACATGATG	CCTCAACCTAAGACACCTTCA
6	BPPCT009	ATTCGGGTCGAACTCCCT	ACGAGCACTAGAGTAACCCTC
7	BPPCT010	AAAGCACAGCCCATAATGC	GTACTGTTACTGCTGGGAATGC
8	BPPCT012	ACTTCCATTGTCAGGCATCA	GGAGCAACGATGGAGTGC
9	BPPCT017	TTAAGAGTTTGTGATGGGAACC	AAGCATAATTTAGCATAACCAAG
10	BPPCT020	CGTGGATGGTCAAGATGC	ATTGACGTGGACTTACAGGTG
11	BPPCT024	GAGGAATGTGCCTCTTCTGG	CTCCCGTACGCGTTTACC
12	BPPCT025	TCCTGCGTAGAAGAAGGTAGC	CGACATAAAGTCCAAATGGC
13	BPPCT026	ATACCTTTGCCACTTGCG	TGAGTTGGAAGAAAACGTAACA
14	BPPCT028	TCAAGTTAGCTGAGGATCGC	GAGCTTGCCTATGAGAAGACC
15	BPPCT029	GGACGGACAGAAATGAAGGT	CCTTAACCCACGCAACTCC
16	BPPCT030	AATTGTACTTGCCAATGCTAT	CTGCCTTCTGCTCACACC
17	BPPCT032	TTAAGCCACAACATCCATGAT	AATGGTCTAAGGAGCACACG
18	BPPCT033	GTAGCCGGAGCCGTGTAT	CTAGAACCCTATAAACACATGGC
19	BPPCT034	CTACCTGAAATAAGCAGAGCCAT	CAATGGAGAATGGGGTGC
20	BPPCT036	AAGCAAAGTCCATAAAAACGC	GGACGAAGACGCTCCATT
21	BPPCT037	CATGGAAGAGGATCAAGT	CTTGAAGGTAGTGCCAAA
22	BPPCT040	ATGAGGACGTGTCTGAAT	TGCCAAACCCCTCTTATA
23	BPPCT041	CAATAAGGCATTTGGAGG	CAGCCGAACCAAGGAGAC
24	CPDCT016	GGAAACCTGATTAGGGCACTT	GGTCTGCTATACTGACCTAGGATT
25	CPDCT028	TGAACGTTGCACTCCTTCAC	ACCACCACCATAACCACCAT
26	CPDCT045	TGTGGATCAAGAAAGAGAACCA	AGGTGTGCTTGCACATGTTT
27	CPPCT025	CAATTAGCTAGAGAGAATTATTG	GACAAGAAGCAAGTAGTTTG
28	CPPCT1	TGTATCCCCTCCTCGGATAA	GGCATTAATGGATGATGATGAA
29	CPPCT13	AGACGCAGCACCCAAACTAC	CATTACATCACCGCCAACAA
30	CPPCT22	ACTCGTGGAGCAGACCACTT	ATCCAAGCATGCCAAGAAAG
31	CPPCT26	TGCTTTCCACGCACACTG	GCCAAGCATTGCGTCGTT
32	CPPCT34	GCATTTCGAGAGCTGTATTT	GTCTTACGTGCAGCTTCATT
33	CPPCT35	TCGGTTTTTAAAATTCCAAAAGTT	ACCCTTATTTGCACCCAACA
34	CPPCT42	CTACCCATTAGCCACCAAGC	TCCCAATTCGTTGCAATCTT
35	CPSCT004	GCTCTGAAGCTCTGCATTGA	TTTGAAATGGCTATGGAGTACG
36	CPSCT005	CTGCAAGCACTGCGGATCTC	CCCATATTCCCAACCCATTA
37	CPSCT006	ACAAACCAAGCACCGTCTC	GGGCAAATGCTTACCTGTTC
38	CPSCT011	ATTTGGGTTTGCGACTCAAG	ACTCATCCCTTGCCCTTTCT
39	CPSCT012	ACGGGAGACTTTCCCAGAAG	CTTCTCGTTTCCTCCCTCCT
40	CPSCT018	AGGACATGTGGTCCAACCTC	GGGTTCCCCGTTACTTTCAT
41	CPSCT021	GCCACTTCGGCTAAAAGAGA	TCCATATCTCCTCCTGCTTGA
42	CPSCT022	TGTCTGCCTCTCATCTTAACCA	TTCTTGAGCAGCCCATCTTCT
43	CPSCT023	CGGGTTGACTCAGTTCCTTC	ATTTGAGCAGAAGCCCAGAA
44	CPSCT024	TGGGTCGTCTTCTTTATCGTG	CCTCACCAAAACGGTAGTCAG
45	CPSCT026	TCTCACACGCTTTCGTCAAC	AAAAAGCCAAAAGGGGTTGT
46	CPSCT032	CATCATCATCCTCACCCAAA	TGCTGATCCGTGAGATCTTG
47	CPSCT033	TCCTCATTTGAGTGTTGTGGA	TGCCCAATTTGAAAACTTTGT
48	CPSCT034	AGGTGGACAATAGCCGTGAT	TTTCCAGACCCTGAGAAAGC
49	CPSCT036	TCGAAGACAGACCAGACAAAGA	TTGCCTTATGCCGGTAATTT
50	CPSCT039	GCCGCAACTCGTAAGGAATA	TCCACCGTTGATTACCCTTC
51	EPDCU5100	CTCTTCTCGCCTCCCAATTT	TGCTTAGCCCTGGGTACAAG
52	GS1	CCACCTGCAACCTTTTTCCC	ATGTTTCCACTGCCTGCCTT
53	PceGA025	GTTGTGTTTACTTTTTTCTTAACGG	GTATCACAAGTGAGAACATAAGAGG
54	PchcmS1	GCAATTCGAGCTGTATTTCAGATG	CAGTTGGCGGCTATCATGTCTTAC
55	PchcmS2	GTTACACCTCTGTCACA	CTTGGCTGGCATTCCTA
56	PchcmS4	AGGGTCGTCTCTTTGAC	CTTCGTTTCAAGGCCTG
57	PchgmS2	CTCACGCTATTTCTCGG	CCTCGACGAAGAGCTCG
58	PchgmS4	GCTAATGAGTTCAGTGTCTACACTC	AATCATAACATCATTCAGCCACTGC
59	PchgmS6	ATCTTCACAACCCTAATG	GTTGAGGCAAAAGACTTC
60	PF1	CATTGTTCATGGGAGGAATT	AGAACATTCCTAAAGGAGCA
61	PGS1.21	CACGAGTGCCAATCCCACC	TTCTTCCGGAAGACCTTGGC
62	PGS1.24	CCCTGGTGTTCTGCTCTCTC	CATCCACAAATGGGAAGCAT
63	PS0lH03	GTAAATGAGTGCCTGCGTGT	TGCGAGAGTTGTGATTGATG
64	PS08E08	TGAGGAGCATAATGACAGT	TCACCATGTGTCATACT
65	PS12A02	CCCAATGAACAACTGCAT	CATATCAATCACTGGGAT
66	SsrPaCITA5	GCCACCAATGGTTCTTCC	AGCACCAGATGCACCTGA
67	UDP96-003	TTGCTCAAAAGTGTCGTTGC	ACACGTAGTGCAACACTGGC
68	UDP96-005	GTAACGCTCGCTACCACAAA	CCTGCATATCACCACCCAG
69	UDP96-008	TTGTACACACCCTCAGCCTG	TGCTGAGGTTCAGGTGAGTG
70	UDP98-022	CTAGTTGTGCACACTCACGC	GTCGCAGGAACAGTAAGCCT
71	UDP98-024	CCTTGATGCATAATCAAACAGC	GGACACACTGGCATGTGAAG
72	UDP98-025	GGGAGGTTACTATGCCATGAAT	CGCAGACATGTAGGACCTC
73	UDP98-405	ACGTGATGAACTGACACCCA	GAGTCTTTGCTCTGCCATCC
74	UDP98-406	TCGGAAACTGGTAGTATGAACAGA	ATGGGTCGTATGCACAGTCA
75	UDP98-409	GCTGATGGGTTTTATGGTTTTC	CGGACTCTTATCCTCTATCAACA
76	UDP98-410	AATTTACCTATCAGCCTCAAA	TTTATGCAGTTTACAGACCG
77	UDP98-412	AGGGAAAGTTTCTGCTGCAC	GCTGAAGACGACGATGATGA
78	UDP98-416	TTTTCTCAGCAGCCAAACAA	ATGTTTCGTGCTTCTGCTCC

编号 No.	引物名 Primer name	正向引物序列（5′-3′） Forward primer sequence	反向引物序列（5′-3′） Reverse primer sequence
1	BPPCT001	AATTCCCAAAGGATGTGTATGAG	CAGGTGAATGAGCCAAAGC
2	BPPCT002	TCGACAGCTTGATCTTGACC	CAATGCCTACGGAGATAAAAGAC
3	BPPCT004	CTGAGTGATCCATTTGCAGG	AGGGCATCTAGACCTCATTGTT
4	BPPCT007	TCATTGCTCGTCATCAGC	CAGATTTCTGAAGTTAGCGGTA
5	BPPCT008	ATGGTGTGTATGGACATGATG	CCTCAACCTAAGACACCTTCA
6	BPPCT009	ATTCGGGTCGAACTCCCT	ACGAGCACTAGAGTAACCCTC
7	BPPCT010	AAAGCACAGCCCATAATGC	GTACTGTTACTGCTGGGAATGC
8	BPPCT012	ACTTCCATTGTCAGGCATCA	GGAGCAACGATGGAGTGC
9	BPPCT017	TTAAGAGTTTGTGATGGGAACC	AAGCATAATTTAGCATAACCAAG
10	BPPCT020	CGTGGATGGTCAAGATGC	ATTGACGTGGACTTACAGGTG
11	BPPCT024	GAGGAATGTGCCTCTTCTGG	CTCCCGTACGCGTTTACC
12	BPPCT025	TCCTGCGTAGAAGAAGGTAGC	CGACATAAAGTCCAAATGGC
13	BPPCT026	ATACCTTTGCCACTTGCG	TGAGTTGGAAGAAAACGTAACA
14	BPPCT028	TCAAGTTAGCTGAGGATCGC	GAGCTTGCCTATGAGAAGACC
15	BPPCT029	GGACGGACAGAAATGAAGGT	CCTTAACCCACGCAACTCC
16	BPPCT030	AATTGTACTTGCCAATGCTAT	CTGCCTTCTGCTCACACC
17	BPPCT032	TTAAGCCACAACATCCATGAT	AATGGTCTAAGGAGCACACG
18	BPPCT033	GTAGCCGGAGCCGTGTAT	CTAGAACCCTATAAACACATGGC
19	BPPCT034	CTACCTGAAATAAGCAGAGCCAT	CAATGGAGAATGGGGTGC
20	BPPCT036	AAGCAAAGTCCATAAAAACGC	GGACGAAGACGCTCCATT
21	BPPCT037	CATGGAAGAGGATCAAGT	CTTGAAGGTAGTGCCAAA
22	BPPCT040	ATGAGGACGTGTCTGAAT	TGCCAAACCCCTCTTATA
23	BPPCT041	CAATAAGGCATTTGGAGG	CAGCCGAACCAAGGAGAC
24	CPDCT016	GGAAACCTGATTAGGGCACTT	GGTCTGCTATACTGACCTAGGATT
25	CPDCT028	TGAACGTTGCACTCCTTCAC	ACCACCACCATAACCACCAT
26	CPDCT045	TGTGGATCAAGAAAGAGAACCA	AGGTGTGCTTGCACATGTTT
27	CPPCT025	CAATTAGCTAGAGAGAATTATTG	GACAAGAAGCAAGTAGTTTG
28	CPPCT1	TGTATCCCCTCCTCGGATAA	GGCATTAATGGATGATGATGAA
29	CPPCT13	AGACGCAGCACCCAAACTAC	CATTACATCACCGCCAACAA
30	CPPCT22	ACTCGTGGAGCAGACCACTT	ATCCAAGCATGCCAAGAAAG
31	CPPCT26	TGCTTTCCACGCACACTG	GCCAAGCATTGCGTCGTT
32	CPPCT34	GCATTTCGAGAGCTGTATTT	GTCTTACGTGCAGCTTCATT
33	CPPCT35	TCGGTTTTTAAAATTCCAAAAGTT	ACCCTTATTTGCACCCAACA
34	CPPCT42	CTACCCATTAGCCACCAAGC	TCCCAATTCGTTGCAATCTT
35	CPSCT004	GCTCTGAAGCTCTGCATTGA	TTTGAAATGGCTATGGAGTACG
36	CPSCT005	CTGCAAGCACTGCGGATCTC	CCCATATTCCCAACCCATTA
37	CPSCT006	ACAAACCAAGCACCGTCTC	GGGCAAATGCTTACCTGTTC
38	CPSCT011	ATTTGGGTTTGCGACTCAAG	ACTCATCCCTTGCCCTTTCT
39	CPSCT012	ACGGGAGACTTTCCCAGAAG	CTTCTCGTTTCCTCCCTCCT
40	CPSCT018	AGGACATGTGGTCCAACCTC	GGGTTCCCCGTTACTTTCAT
41	CPSCT021	GCCACTTCGGCTAAAAGAGA	TCCATATCTCCTCCTGCTTGA
42	CPSCT022	TGTCTGCCTCTCATCTTAACCA	TTCTTGAGCAGCCCATCTTCT
43	CPSCT023	CGGGTTGACTCAGTTCCTTC	ATTTGAGCAGAAGCCCAGAA
44	CPSCT024	TGGGTCGTCTTCTTTATCGTG	CCTCACCAAAACGGTAGTCAG
45	CPSCT026	TCTCACACGCTTTCGTCAAC	AAAAAGCCAAAAGGGGTTGT
46	CPSCT032	CATCATCATCCTCACCCAAA	TGCTGATCCGTGAGATCTTG
47	CPSCT033	TCCTCATTTGAGTGTTGTGGA	TGCCCAATTTGAAAACTTTGT
48	CPSCT034	AGGTGGACAATAGCCGTGAT	TTTCCAGACCCTGAGAAAGC
49	CPSCT036	TCGAAGACAGACCAGACAAAGA	TTGCCTTATGCCGGTAATTT
50	CPSCT039	GCCGCAACTCGTAAGGAATA	TCCACCGTTGATTACCCTTC
51	EPDCU5100	CTCTTCTCGCCTCCCAATTT	TGCTTAGCCCTGGGTACAAG
52	GS1	CCACCTGCAACCTTTTTCCC	ATGTTTCCACTGCCTGCCTT
53	PceGA025	GTTGTGTTTACTTTTTTCTTAACGG	GTATCACAAGTGAGAACATAAGAGG
54	PchcmS1	GCAATTCGAGCTGTATTTCAGATG	CAGTTGGCGGCTATCATGTCTTAC
55	PchcmS2	GTTACACCTCTGTCACA	CTTGGCTGGCATTCCTA
56	PchcmS4	AGGGTCGTCTCTTTGAC	CTTCGTTTCAAGGCCTG
57	PchgmS2	CTCACGCTATTTCTCGG	CCTCGACGAAGAGCTCG
58	PchgmS4	GCTAATGAGTTCAGTGTCTACACTC	AATCATAACATCATTCAGCCACTGC
59	PchgmS6	ATCTTCACAACCCTAATG	GTTGAGGCAAAAGACTTC
60	PF1	CATTGTTCATGGGAGGAATT	AGAACATTCCTAAAGGAGCA
61	PGS1.21	CACGAGTGCCAATCCCACC	TTCTTCCGGAAGACCTTGGC
62	PGS1.24	CCCTGGTGTTCTGCTCTCTC	CATCCACAAATGGGAAGCAT
63	PS0lH03	GTAAATGAGTGCCTGCGTGT	TGCGAGAGTTGTGATTGATG
64	PS08E08	TGAGGAGCATAATGACAGT	TCACCATGTGTCATACT
65	PS12A02	CCCAATGAACAACTGCAT	CATATCAATCACTGGGAT
66	SsrPaCITA5	GCCACCAATGGTTCTTCC	AGCACCAGATGCACCTGA
67	UDP96-003	TTGCTCAAAAGTGTCGTTGC	ACACGTAGTGCAACACTGGC
68	UDP96-005	GTAACGCTCGCTACCACAAA	CCTGCATATCACCACCCAG
69	UDP96-008	TTGTACACACCCTCAGCCTG	TGCTGAGGTTCAGGTGAGTG
70	UDP98-022	CTAGTTGTGCACACTCACGC	GTCGCAGGAACAGTAAGCCT
71	UDP98-024	CCTTGATGCATAATCAAACAGC	GGACACACTGGCATGTGAAG
72	UDP98-025	GGGAGGTTACTATGCCATGAAT	CGCAGACATGTAGGACCTC
73	UDP98-405	ACGTGATGAACTGACACCCA	GAGTCTTTGCTCTGCCATCC
74	UDP98-406	TCGGAAACTGGTAGTATGAACAGA	ATGGGTCGTATGCACAGTCA
75	UDP98-409	GCTGATGGGTTTTATGGTTTTC	CGGACTCTTATCCTCTATCAACA
76	UDP98-410	AATTTACCTATCAGCCTCAAA	TTTATGCAGTTTACAGACCG
77	UDP98-412	AGGGAAAGTTTCTGCTGCAC	GCTGAAGACGACGATGATGA
78	UDP98-416	TTTTCTCAGCAGCCAAACAA	ATGTTTCGTGCTTCTGCTCC

性状 Trait	年份 Year	最大值 Max	最小值 Min	平均值 Mean	极差 Range	标准差 SD	变异系数/% CV	偏度 Skew.	峰度 Kurt.
LD/mm	2022	53.10	27.04	35.61	26.06	6.53	18.33	0.75	-0.21
	2023	54.50	26.54	37.14	27.96	6.17	16.60	0.39	-0.23
TD/mm	2022	53.12	29.90	40.34	23.22	6.56	16.25	0.18	-1.15
	2023	61.81	28.90	41.65	32.91	6.43	15.44	0.15	-0.19
FS	2022	1.08	0.66	0.90	0.42	0.07	8.09	-0.09	0.11
	2023	1.04	0.78	0.89	0.26	0.07	7.32	0.28	-0.80
FW/g	2022	87.05	13.97	35.64	73.08	17.32	48.59	0.78	-0.06
	2023	89.11	13.13	38.40	75.98	16.87	43.92	0.73	0.47
EA/%	2022	0.98	0.93	0.97	0.05	0.01	1.34	-1.12	0.62
	2023	0.99	0.93	0.97	0.06	0.01	1.31	-1.01	0.71
FH/N	2022	19.46	1.72	11.68	17.74	4.57	39.11	-0.50	-0.59
	2023	21.81	0.82	11.12	20.99	5.15	46.33	-0.04	-0.66
TS/%	2022	13.20	6.46	9.63	6.74	1.46	15.20	-0.08	0.03
	2023	13.21	6.32	9.63	6.89	1.53	15.91	-0.11	-0.41
RS/%	2022	9.05	4.23	6.89	4.82	1.09	15.82	-0.30	-0.23
	2023	9.49	3.01	7.04	6.48	1.34	18.99	-0.49	-0.15
VC/（μg · g^-1）	2022	67.6	13.9	38.9	53.7	11.0	28.34	0.11	-0.12
	2023	71.3	9.6	40.4	61.7	13.9	34.48	0.08	-0.32
TSS/%	2022	16.70	8.60	12.61	8.10	1.72	13.62	-0.25	0.02
	2023	16.90	6.60	12.31	10.30	2.13	17.29	-0.30	-0.18
TA/%	2022	1.43	0.49	0.95	0.94	0.18	18.69	-0.07	0.03
	2023	1.71	0.42	0.93	1.29	0.24	25.79	0.38	0.36

性状 Trait	年份 Year	最大值 Max	最小值 Min	平均值 Mean	极差 Range	标准差 SD	变异系数/% CV	偏度 Skew.	峰度 Kurt.
LD/mm	2022	53.10	27.04	35.61	26.06	6.53	18.33	0.75	-0.21
	2023	54.50	26.54	37.14	27.96	6.17	16.60	0.39	-0.23
TD/mm	2022	53.12	29.90	40.34	23.22	6.56	16.25	0.18	-1.15
	2023	61.81	28.90	41.65	32.91	6.43	15.44	0.15	-0.19
FS	2022	1.08	0.66	0.90	0.42	0.07	8.09	-0.09	0.11
	2023	1.04	0.78	0.89	0.26	0.07	7.32	0.28	-0.80
FW/g	2022	87.05	13.97	35.64	73.08	17.32	48.59	0.78	-0.06
	2023	89.11	13.13	38.40	75.98	16.87	43.92	0.73	0.47
EA/%	2022	0.98	0.93	0.97	0.05	0.01	1.34	-1.12	0.62
	2023	0.99	0.93	0.97	0.06	0.01	1.31	-1.01	0.71
FH/N	2022	19.46	1.72	11.68	17.74	4.57	39.11	-0.50	-0.59
	2023	21.81	0.82	11.12	20.99	5.15	46.33	-0.04	-0.66
TS/%	2022	13.20	6.46	9.63	6.74	1.46	15.20	-0.08	0.03
	2023	13.21	6.32	9.63	6.89	1.53	15.91	-0.11	-0.41
RS/%	2022	9.05	4.23	6.89	4.82	1.09	15.82	-0.30	-0.23
	2023	9.49	3.01	7.04	6.48	1.34	18.99	-0.49	-0.15
VC/（μg · g^-1）	2022	67.6	13.9	38.9	53.7	11.0	28.34	0.11	-0.12
	2023	71.3	9.6	40.4	61.7	13.9	34.48	0.08	-0.32
TSS/%	2022	16.70	8.60	12.61	8.10	1.72	13.62	-0.25	0.02
	2023	16.90	6.60	12.31	10.30	2.13	17.29	-0.30	-0.18
TA/%	2022	1.43	0.49	0.95	0.94	0.18	18.69	-0.07	0.03
	2023	1.71	0.42	0.93	1.29	0.24	25.79	0.38	0.36

引物名称 Primer name	条带数 Number of strips	多态性条带数 Polymorp-hic bands	等位基因数 N_a	主要等位基因频率 Major allele frquency	基因多样性 H_e	多态性信息含量 PIC
BPPCT001	8	6	8	0.43	0.76	0.72
BPPCT002	5	3	5	0.81	0.12	0.31
BPPCT004	10	6	7	0.38	0.37	0.74
BPPCT007	15	11	9	0.38	0.95	0.75
BPPCT008	7	4	4	0.40	0.99	0.64
BPPCT009	9	8	9	0.36	0.96	0.71
BPPCT010	6	4	5	0.45	0.89	0.64
BPPCT012	6	4	4	0.40	0.93	0.64
BPPCT017	5	3	4	0.43	0.50	0.65
BPPCT020	9	3	4	0.42	0.35	0.64
BPPCT024	11	7	8	0.42	0.99	0.66
BPPCT025	9	6	7	0.44	0.95	0.58
BPPCT026	9	8	9	0.44	0.93	0.69
BPPCT028	12	7	8	0.37	0.97	0.72
BPPCT029	8	6	7	0.27	0.84	0.78
BPPCT030	7	6	7	0.69	0.11	0.44
BPPCT032	7	2	3	0.80	0.20	0.31
BPPCT033	10	7	5	0.49	0.98	0.55
BPPCT034	8	3	4	0.38	0.87	0.61
BPPCT036	6	5	5	0.49	0.98	0.46
BPPCT037	7	3	5	0.65	0.21	0.51
BPPCT040	7	5	6	0.45	0.78	0.62
BPPCT041	5	3	4	0.70	0.29	0.45
CPDCT016	8	2	4	0.77	0.32	0.34
CPDCT028	4	2	4	0.52	0.64	0.54
CPDCT045	7	3	4	0.51	0.35	0.57
CPPCT025	4	4	4	0.51	0.82	0.54
CPPCT1	8	6	6	0.48	0.79	0.57
CPPCT13	8	6	7	0.26	0.93	0.80
CPPCT22	6	2	4	0.57	0.20	0.51
CPPCT26	7	5	6	0.41	0.91	0.65
CPPCT34	4	4	5	0.56	0.24	0.56
CPPCT35	10	8	9	0.26	0.81	0.78
CPPCT42	8	5	6	0.45	0.99	0.54
CPSCT004	8	4	5	0.38	0.91	0.64
CPSCT005	10	7	6	0.38	0.93	0.73
CPSCT006	10	6	7	0.39	0.91	0.63
CPSCT011	8	6	7	0.36	0.96	0.73
CPSCT012	7	5	6	0.49	0.94	0.59
CPSCT018	10	4	4	0.39	0.95	0.65
CPSCT021	8	5	5	0.47	0.97	0.62
CPSCT022	5	5	6	0.36	0.89	0.71
CPSCT023	6	4	5	0.52	0.85	0.46
CPSCT024	9	3	4	0.41	0.84	0.59
CPSCT026	7	5	6	0.38	0.89	0.69
CPSCT032	6	3	4	0.52	0.72	0.52
CPSCT033	5	4	5	0.46	0.96	0.64
CPSCT034	6	4	4	0.82	0.25	0.29
CPSCT036	8	2	3	0.90	0.20	0.17
CPSCT039	7	5	6	0.42	0.96	0.66
EPDCU5100	7	2	3	0.87	0.11	0.23
GS1	6	5	6	0.44	0.41	0.68
PceGA025	7	3	5	0.53	0.20	0.59
PchcmS1	3	1	3	0.82	0.00	0.26
PchcmS2	2	1	3	0.83	0.00	0.27
PchcmS4	4	2	3	0.95	0.10	0.09
PchgmS2	5	3	4	0.59	0.60	0.47
PchgmS4	6	4	4	0.74	0.30	0.40
PchgmS6	7	5	6	0.33	0.78	0.76
PF1	2	1	2	0.96	0.00	0.07
PGS1.21	5	4	6	0.55	0.39	0.58
PGS1.24	5	2	4	0.78	0.01	0.31
PS0lH03	6	3	5	0.88	0.05	0.21
PS08E08	5	3	4	0.84	0.15	0.27
PS12A02	7	2	3	0.77	0.12	0.31
SsrPaCITA5	4	1	3	0.97	0.00	0.06
UDP96-003	6	3	6	0.48	0.95	0.45
UDP96-005	5	4	5	0.47	0.88	0.53
UDP96-008	6	4	6	0.57	0.22	0.59
UDP98-022	8	1	8	0.46	0.00	0.65
UDP98-024	8	2	4	0.85	0.05	0.25
UDP98-025	6	5	6	0.41	0.87	0.70
UDP98-405	5	3	5	0.77	0.16	0.37
UDP98-406	6	2	4	0.64	0.20	0.51
UDP98-409	11	4	6	0.50	0.21	0.60
UDP98-410	7	2	3	0.87	0.01	0.22
UDP98-412	6	4	5	0.60	0.76	0.49
UDP98-416	2	1	4	0.56	0.43	0.53
平均值Mean	6.88	4.05	5.19	0.55	0.56	0.53