薰衣草CBF途径相关耐寒基因挖掘与调控网络分析

doi:10.16420/j.issn.0513-353x.2021-0888

摘要/Abstract

摘要：

为探究薰衣草（Lavandula angustifolia Mill.）的耐冷机制，使用转录组学方法对薰衣草响应冷处理的分子调控网络进行挖掘分析。CBF转录因子和其靶基因COR形成的CBF-COR调控网络在增强植物耐冷胁迫中作用显著。本研究中共发现7个CBF基因（LaCBF）和11个COR基因（LaCOR）在冷处理下的表达具有显著差异，同时在CBF信号调控通路中发现4个上游基因LaICE1、LaCAMTA3、LaEBF1、LaCCA1对冷胁迫有响应，认为这些基因共同形成的冷信号调控网络在薰衣草抵御低温中起到了重要作用。

关键词: 薰衣草, 低温胁迫, 转录组学分析, CBF转录因子

Abstract:

To explore the cold-tolerant mechanisms of lavender（Lavandula angustifolia Mill.），transcriptomics methods were used for discovering and analyzing the molecular regulation networks in responses to cold treatments in this research. The CBF-COR regulation networks composed by CBF transcription factors and their target genes COR played significant roles in improving plant cold-tolerance. In this research，seven CBF genes（LaCBF）and 11 COR genes（LaCOR）were found with significant expression difference under cold stress. Meanwhile，four upstream genes including LaICE1，LaCAMTA3，LaEBF1，and LaCCA1 in CBF regulation pathways were found to be responsive to cold stress. It is considered that the cold signal regulation networks together formed by these genes took the key roles in resistance to low temperature in Lavandula angustifolia.

Key words: Lavandula angustifolia, low temperature stress, transcriptomics analysis, CBF transcription factors

中图分类号:

蔺海娇, 梁雨晨, 李玲, 马军, 张璐, 兰振颖, 苑泽宁. 薰衣草CBF途径相关耐寒基因挖掘与调控网络分析[J]. 园艺学报, 2023, 50(1): 131-144.

LIN Haijiao, LIANG Yuchen, LI Ling, MA Jun, ZHANG Lu, LAN Zhenying, YUAN Zening. Exploration and Regulation Network Analysis of CBF Pathway Related Cold Tolerance Genes in Lavandula angustifolia[J]. Acta Horticulturae Sinica, 2023, 50(1): 131-144.

图/表 10

表1 qPCR反应所用引物

Table 1 Primers used for qPCR

序号 ID	引物序列（5′-3′） Primers sequence
1	CCA1-F:TGCCGGGAAAGAGGAGTACT	CCA1-R:CCCACTTCGTTAGGAGGCAA
2	LHY-F:TGGGTAATTGGGTCAAGGCC	LHY-R:TTCCGGGTCTTTCTCCATGC
3	ICE1-F:TTGAGCTGCGACCAGAGTTT	ICE1-R:CAGCTTAGACCTGTGCGGAT
4	CBF1-F:TGCAGCGGAGGCTTTCTATC	CBF1-R:AAGCCTTCAGCCATGTTGGA
5	CBF2-F:CGATACAGGCAAGTGGGTCT	CBF2-R:AATGTCCTGCACACTCGACG
6	CBF3-F:GTGCTGGAATTCTCCTCCGA	CBF3-R:TGACCTCGCAGACCCACTTA
7	ERD7-F:TGGTGAGGAAGAAGGATGCG	ERD7-R:CCACTTCAGCCTATCCACCG
8	COR413PM2-F:AGAGGCGGGGTAGTCATCTT	COR413PM2-R:GTCAGTGCTCGTGTGTGAGA
9	GAPDH-F:TAGGAGGTGGCAGGACATCA	GAPDH-R:CCCTTTACCCGTCACGTTGT

图1 0 ℃胁迫下薰衣草转录因子差异表达基因火山图

Fig. 1 Volcano map of transcription factor differentially expressed genes in Lavandula angustifolia under 0 ℃ stress

图2 薰衣草转录因子差异表达基因GO统计柱形图

Fig. 2 GO statistical bar chart of transcription factor differentially expressed genes in Lavandula angustifolia

图3 薰衣草CBF-COR调控网络相关耐寒基因聚类图

Fig. 3 Cluster map of cold tolerance genes associated with CBF-COR regulatory network in Lavandula angustifolia

图4 薰衣草CBF-COR调控网络相关耐寒基因GO弦图

Fig. 4 GO chordal graph of cold tolerance genes associated with CBF-COR regulatory network in Lavandula angustifolia

图5 薰衣草CBF途径相关耐寒基因与拟南芥数据库比对的蛋白网络互作图

Fig. 5 Mapping of protein network interactions between CBF pathway related cold tolerance genes in Lavandula angustifolia and Arabidopsis thaliana database

图6 薰衣草与拟南芥中CBF转录因子系统发育树

Fig. 6 Phylogenetic tree of CBF transcription factors in Lavandula angustifolia and Arabidopsis thaliana

图7 不同温度下薰衣草COR基因表达变化不同字母表示差异显著（P < 0.05）。

Fig. 7 Changes of COR gene expression in Lavandula angustifolia at different temperatures Different letters indicated significant difference（P < 0.05）.

图8 转录组测序与qPCR基因表达水平的比较

Fig. 8 Comparison of gene expression levels between RNA-seq and qPCR

图9 薰衣草响应低温胁迫的CBF-COR调控网络根据Shi等（2018）修改绘制。

Fig. 9 CBF-COR regulatory network in response to low temperature stress in Lavandula angustifolia Modified and drawn according to the literature Shi et al.（2018）.

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