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园艺学报 ›› 2022, Vol. 49 ›› Issue (4): 868-874.doi: 10.16420/j.issn.0513-353x.2021-0198

• 研究报告 • 上一篇    下一篇

马铃薯与枸杞嫁接愈合过程的解剖学观察

祁利潘1,2, 李越2, 王磊2, 冯琰2, 王宽2, 尹江2, 郭华春1,**()   

  1. 1.云南农业大学薯类研究所,昆明 650201
    2.河北北方学院旱作农业研究中心,河北张家口 075000
  • 收稿日期:2021-09-28 修回日期:2022-01-18 出版日期:2022-04-25 发布日期:2022-04-24
  • 通讯作者: 郭华春 E-mail:ynghc@126.com
  • 基金资助:
    国家现代农业产业技术体系建设专项资金项目(CARS-09-P05);河北省科技计划项目(16227508D)

Anatomical Observation on the Graft Union Between Potato and Wolfberry

QI Lipan1,2, LI Yue2, WANG Lei2, FENG Yan2, WANG Kuan2, YIN Jiang2, GUO Huachun1,**()   

  1. 1. Tuber and Root Crop Research Institute,Yunnan Agricultural University,Kunming 650201,China
    2. Rain-fed Agricultrue Research Center,Hebei North University,Zhangjiakou,Hebei 075000,China
  • Received:2021-09-28 Revised:2022-01-18 Online:2022-04-25 Published:2022-04-24
  • Contact: GUO Huachun E-mail:ynghc@126.com

摘要:

为揭示马铃薯与枸杞嫁接愈合过程,采用石蜡切片技术对马铃薯/枸杞嫁接植株进行解剖学观察。结果显示:马铃薯/枸杞嫁接植株生长旺盛,可完成整个生育进程。马铃薯/枸杞嫁接愈合过程可分为4个阶段:(1)隔离层产生期,嫁接5 d后,嫁接接口处产生隔离层;(2)愈伤组织形成期,嫁接9 d后,隔离层细胞脱分化,最后形成愈伤组织;(3)愈伤组织分化期,嫁接13 d后,愈伤组织分化,形成愈伤组织桥;(4)新的维管组织形成期,嫁接17 d后,形成新的韧皮部和木质部及维管束桥,使砧木和接穗完全结合,愈合过程结束。

关键词: 马铃薯, 枸杞, 石蜡切片, 嫁接愈合

Abstract:

In order to reveal the graft union process between potato and wolfberry,anatomical observation of potato/wolfberry grafting system was carried out using paraffin section technique. The results showed that the potato/wolfberry grafting system grew vigorously and could complete the entire reproductive process. The union process of potato/wolfberry grafting could be divided into four stages:(1) isolation layer generation period,5 d after grafting,an isolation layer was formed at the grafting interface;(2) callus formation period,9 d after grafting,the isolation layer cells dedifferentiated,finally callus was formed;(3) callus differentiation stage,13 d after grafting,the callus differentiated,forming callus bridge;(4) new vascular tissue formation period,17 d after grafting,new phloem,xylem and vascular bundle bridges were formed which made the rootstock and scion fully combined,and the union process was finished.

Key words: potato, wolfberry, paraffin section, grafting union

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