关键词: 百合, 深绿木霉T2, TraT2A, 气孔, 光响应, 荧光特性, 灰霉病, 诱导抗性

Abstract: Lanzhou lily and Trichoderma atroviride T2 proteinaceous TraT2A were used as experimental materials to explore the physiological mechanisms of TraT2A in the resistance of Lanzhou lily to gray mold. The changes in the physiological characteristics of stomata， photosynthetic- photoresponsiveness，chlorophyll fluorescence of the lily leaves were analyzed，and the resistance of the lily against Botrytis cinerea was measured after a combinatorial treatment of TraT2A and inoculation of Botrytis cinerea（IB）. The results showed that the stomatal aperture of the lily leaves became smaller by the TraT2A + IB treatment. With the enhancement of photosynthetically active radiation（PAR），the electron transport rate（ETR）of TraT2A + IB treated lily leaves was significantly higher than that of the control，inoculation of Botrytis cinerea and TraT2A treatment. After TraT2A + IB treatment，the maximum photon yield（Fv/Fm），PSⅡ potential activity（Fv/F0），ΦPSⅡ and qP of PSⅡ were higher，and the NPQ value was lower than those of the control，IB and TraT2A，and they were significantly different. The induction effect of lily against Botrytis cinerea reached above 96% in 5 d and 7 d after the TraT2A + IB treatment. The results indicate that the PSⅡ reaction center could capture light energy more effectively in photosynthesis and thus enhance the photosynthetic characteristics and the disease resistance to gray mold in the TraT2A + IB-treated Lanzhou lily.

Key words: lily, Trichoderma atroviride T2, TraT2A, stomata, light response, fluorescence characteristics, gray mold, induced resistance