园艺学报 ›› 2013, Vol. 40 ›› Issue (3): 540-548.

• 研究报告 • 上一篇    下一篇


程 鸿,孔维萍,何启伟,王晓巍   

  1. (1 甘肃省农业科学院蔬菜研究所,农业部园艺作物生物学与种质创制西北地区科学观测实验站,兰州 730070;2 山
    东省农业科学院蔬菜研究所,济南 250100)
  • 出版日期:2013-03-25 发布日期:2013-03-25

CmMLO2:A Novel Gene Closely Associated with the Powdery Mildew in

 CHENG   Hong, KONG  Wei-Ping, HE  Qi-Wei, WANG  Xiao-Wei   

  1. (1Institute of Vegetable,Gansu Academy of Agricultural Science,Lanzhou Research Station of Horticultural Crop Biology
    and Germplasm Enhancement,Ministry of Agriculture,P. R. China,Lanzhou 730070,China;2Institute of Vegetable,
    Shandong Academy of Agricultural Science,Ji’nan 250100,China)
  • Online:2013-03-25 Published:2013-03-25

摘要: 通过RT-PCR 方法从甜瓜中克隆得到白粉病感病相关基因CmMLO2 的cDNA 序列,GenBank 登录号为
FJ713542。该基因ORF 全长1 713 bp,可编码由570 个氨基酸组成的蛋白质,具有7 个跨膜螺旋结构,属于典型的
跨膜蛋白。RT-PCR 结果表明,CmMLO2 主要在甜瓜叶片中表达,具有组织特异性。在受到白粉病菌胁迫时CmMLO2
表达显著上调,表明CmMLO2 很有可能与白粉病发病有关。构建RNAi 表达载体pFGC1008-CmMLO2 后,利用叶
盘转化法转化甜瓜,获得了PCR 阳性植株,白粉病接种鉴定结果表明,转化植株对白粉病具有抗性,证明通过ihpRNAi

关键词: 甜瓜, 白粉病, MLO, 基因表达, 遗传转化

Abstract: The full-length cDNA sequence of the MLO gene was cloned via RACE-PCR from
muskmelon(Cucumis melo L.),and was designated as CmMLO2(GenBank Accession No. FJ713542).
The gene is 1 713 bp long and encodes a 570-amino acid peptide with a seven-transmembrane domain
topology,and is a typical transmembrane protein. Its expression pattern was analyzed using quantitative
real-time PCR after the melon leaf was infected with powdery mildew. The results indicate that CmMLO2
is mainly expressed in melon leaves in a tissue-specific distribution. Moreover,CmMLO2 may play a
crucial role in the pathogenesis of powdery mildew. After the efficient of dsRNA constructs were
examined via pFGC1008-CmMLO2 were introduced into Agrobacterium tumefaciens and transformed into
melon wild type with the methods of Agrobacterium-mediated leaf disc transformation. The material of
broad-spectrum powdery mildew resistance against P. xanthii in muskmelon was obtained by
ihpRNAi-mediated knock-down of CmMLO2.

Key words: melon, powdery mildew, MLO, gene expression, genetic transformation