https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2012, Vol. 39 ›› Issue (2): 265-272.

• 果树 • 上一篇    下一篇

火龙果Ty1-copia类反转录转座子反转录酶序列的克隆及分析

范付华,乔  光,郑思成,文晓鹏*   

  1. 贵州大学贵州省农业生物工程重点实验室,贵阳 550025
  • 收稿日期:2011-10-20 修回日期:2012-01-05 出版日期:2012-02-25 发布日期:2012-02-25
  • 通讯作者: 文晓鹏 E-mail:xpwensc@hotmail.com

Cloning and Analysis of Reverse Transcriptase of Ty1-copia Retrotrans- posons in Hylocereus undatus

FAN Fu-hua,QIAO Guang,ZHENG Si-cheng,and WEN Xiao-peng*   

  1. Guizhou Key Laboratory of Agricultural Bioengineering,Guizhou University,Guiyang 550025,China
  • Received:2011-10-20 Revised:2012-01-05 Online:2012-02-25 Published:2012-02-25
  • Contact: WEN Xiao-peng E-mail:xpwensc@hotmail.com

摘要: 根据Ty1-copia类反转录转座子反转录酶的保守区设计简并引物,通过PCR扩增,从火龙果基因组中扩增出260 bp左右的目标条带,经回收、克隆、测序及序列分析,获得了23条来源于火龙果试管苗变异和非变异材料的反转录酶序列。通过系统演化分析,所得序列可分为5类,存在较高的异质性;与母株相比,变异植株的反转录酶序列存在缺失、移码和终止密码子突变。经反转录酶氨基酸序列对比,火龙果与其它物种间具有较高的同源性,表明在不同物种间可能存在反转录转座子的横向传递作用。

关键词: 火龙果, 变异, Ty1-copia类反转录转座子, 反转录酶, 异质性

Abstract: Using degenerate oligonucleotide primers corresponding to the conserved domains of the Ty1-copia retrotransposon reverse transcriptase,a fragment of 260 bp was amplified by polymerase chain reaction(PCR)from genomic DNA of Hylocereus undatus. The amplicons were recovered and cloned,then sequenced and analyzed. Twenty-three DNA sequences of reverse transcriptase were obtained from  normal or somaclonal variant of Hylocereus undatus in vitro plants. The sequences can be classified into  five groups with high heterogeneity through phylogenic analysis. The DNA sequences displayed mutation including deletion,frameshift or stop codon. A phylogenic tree was constructed based on the amino acid sequences from other species,indicating that they might share the common origin and that horizontal transmission of retrotransposons has occurred among the plants in the past.

Key words: Hylocereus undatus, variation, Ty1-copia-like retrotransposon, reverse transcriptase, heterogeneity

中图分类号: