外源H2O2作用下大蒜试管苗活性氧代谢的变化及对AsA的响应

园艺学报 ›› 2011, Vol. 38 ›› Issue (09): 1707-1716.

外源H2O2作用下大蒜试管苗活性氧代谢的变化及对AsA的响应

张琳1,2，蒋芳玲1,2,*，熊超超1,2，孙萍萍1,2，靳慧卿1,2，田洁1,2，吴震1,2,**

（1南京农业大学园艺学院，南京210095；2农业部南方蔬菜遗传改良重点开放实验室，南京210095）

收稿日期:2011-06-21 修回日期:2011-08-23 出版日期:2011-09-25 发布日期:2011-09-25
通讯作者: 吴震

Changes of Reactive Oxygen Metabolism of Garlic Plantlet in Vitro Under Exogenous H2O2 Stress and the Responses to AsA

ZHANG Lin1,2，JIANG Fang-ling1,2,*，XIONG Chao-chao1,2，SUN Ping-ping1,2，JIN Hui-qing1,2，TIAN Jie1,2，and WU Zhen1,2,* *

（1College of Horticulture，Nanjing Agricultural University，Nanjing 210095，China；2Key Laboratory of Southern Vegetable Crop Genetic Improvement，Ministry of Agriculture，Nanjing 210095，China）

Received:2011-06-21 Revised:2011-08-23 Online:2011-09-25 Published:2011-09-25
Contact: WU Zhen1

摘要/Abstract

摘要： 探讨外源活性氧物质（ROS）引起试管苗玻璃化，以及活性氧清除剂对试管苗玻璃化的缓解效应和机理，以大蒜品种‘二水早’试管苗为材料，研究外源过氧化氢（H2O2）及H2O2 + 活性氧清除剂抗坏血酸（AsA）处理下试管苗玻璃化及活性氧代谢的变化。结果表明：外源H2O2处理使大蒜试管苗内源超氧阴离子产生速率和H2O2含量提高，玻璃化率和玻璃化程度提高。玻璃化现象的发生较内源大量发生滞后，与H2O2显著积累同步。外源H2O2处理0 ~ 8 d，超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）活性有上升趋势并显著高于对照，但在8 d后抗氧化酶活性或者下降（SOD、CAT），或增加缓慢（POD），而内源抗氧化物质AsA含量在处理8 d时显著低于对照。在外源H2O2处理的同时添加外源AsA，因外源H2O2引起的内源的产生和H2O2的积累减少，SOD、POD、CAT等抗氧化酶活性保持持续上升趋势，并在处理后期高于添加H2O2的处理及对照，内源AsA含量保持较高水平并显著高于外源H2O2处理；同时玻璃化率增加缓慢。综上所述，外源H2O2可提高大蒜试管苗内源的产生速率和H2O2含量，降低抗氧化系统的抗氧化能力，进而导致试管苗玻璃化加重；添加外源抗氧化物质AsA可缓解的产生和H2O2的积累，对试管苗玻璃化发生有控制效果。

关键词: 大蒜, 试管苗, 玻璃化, 抗坏血酸, 过氧化氢, 活性氧

Abstract: n order to investigate the inductive effect of exogenous reactive oxygen species（ROS）on hyperhydricity of plantlet in vitro，as well as the mitigative effect and mechanism of exogenous ROS scavenger on hyperhydricity，using garlic variety‘Ershuizao’as test material，the effects of exogenoushydrogen peroxide（H2O2）and H2O2 + Ascorbic acid（AsA，a ROS scavenger）on hyperhydricity and changes of reactive oxygen metabolism in plantlet in vitro were studied. The results showed that exogenous H2O2 treatment led to the increase of superoxide anion（）production rate and H2O2 content，as well as the rise of hyperhydricity rate and degree in garlic plantlet in vitro. The occurence of hyperhydricity lagged behind substantial generation of endogenous ，and was in synchrony with H2O2 accumulation. Between day 0 to 8 of exposure to H2O2，superoxide dismutase（SOD），peroxidase（POD）and catalase（CAT）activities were on the rise and significantly higher than that of the control. But after 8 d，antioxidant enzymes activities decreased（SOD，CAT）or increased slowly（POD）. Whereas internal antioxidant compound，AsA，content on 8 d was remarkably lower than that of the control. Exogenous H2O2 + AsA treatment could reduce internalproduction rate and H2O2 content caused by exogenous H2O2；Antioxidant enzymes（SOD，POD and CAT etc.）activities kept rising，and were higher than that of the H2O2 treatment and control at the later stage；Endogenous AsA maintained a high level and were significantly higher than that of the exogenous H2O2 treatment；At the same time，hyperhydricity rate of garlic plantlet in vitro raised slowly，and was significantly lower than that of the exogenous H2O2 treatment. In sum，exogenous H2O2 treatment could lead to the increase of production rate and H2O2 accumulation；Reduce the antioxidant capacity of antioxidant system，resulting in the rise of hyperhydricity rate. The addition of exogenous antioxidant，AsA，could relieve the increase of production rate and H2O2 content，and then effectively control the occurrence of the hyperhydricity rate of plantlet in vitro.

Key words: llium sativum L., plantlet in vitro, hyperhydricity, ascorbic acid, hydrogen peroxide, reactive oxygen species

中图分类号:

S 633.4

张琳;蒋芳玲;;熊超超;孙萍萍;靳慧卿;田洁;吴震;. 外源H2O2作用下大蒜试管苗活性氧代谢的变化及对AsA的响应[J]. 园艺学报, 2011, 38(09): 1707-1716.

ZHANG Lin;JIANG Fang-ling;;XIONG Chao-chao;SUN Ping-ping;JIN Hui-qing;TIAN Jie;and WU Zhen;. Changes of Reactive Oxygen Metabolism of Garlic Plantlet in Vitro Under Exogenous H2O2 Stress and the Responses to AsA[J]. ACTA HORTICULTURAE SINICA, 2011, 38(09): 1707-1716.

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