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园艺学报 ›› 2008, Vol. 35 ›› Issue (10): 1511-1516.

• 研究简报 • 上一篇    下一篇

用AFLP标记分析广西龙眼种质遗传多样性

彭宏祥1,李冬波1*,朱建华1,李江舟2,曹辉庆1,潘丽梅2
  

  1. 1广西农业科学院园艺研究所,南宁 530007;2广西大学农学院,南宁 530005)
  • 收稿日期:2008-06-11 修回日期:2008-09-22 出版日期:2008-10-25 发布日期:2008-10-25
  • 通讯作者: 李冬波

Genetic Diversity of Longan in Guangxi Assessed by AFLP Markers

PENG Hong-xiang1;LI Dong-bo1*;ZHU Jian-hua1;LI Jiang-zhou2;CAO Hui-qing1;and PAN Li-mei2
  

  1. (1Horticulture Research Institute , Guangxi Academy of Agricultural Sciences, Nanning 530007, China; 2Agriculture College, Guangxi University, Nanning 530005, China)
  • Received:2008-06-11 Revised:2008-09-22 Online:2008-10-25 Published:2008-10-25
  • Contact: LI Dong-bo

摘要:

选用10对AFLP引物组合分析了广西38个龙眼品种(优良单株)和两个龙眼近缘亚种龙荔单株,以及10个来自国内外其他产区龙眼品种的遗传多样性。在所扩增的570条AFLP主带中,有485条具有多态性,占85.1%。所用引物可将供试的品种(优良单株)区分开来,供试材料的遗传相似系数在0.39(两个亚种间)到0.98之间。根据遗传相似系数(UPGMA, N-J)得到的分类树状图与传统方法的分类结果相类似。结果表明,广西龙眼种质具有较广泛的遗传多样性。

关键词: 龙眼, 遗传多样性, AFLP

Abstract:

To determine the genetic diversity of longan(Dimocarpus longan Lour.) varieties in Guangxi, we employed 10 pairs of AFLP primer combinations on 38 varieties and 2 subspecific varieties〔Dimocarpus confinis (How et Ho) H. S. Do〕 in Guangxi, and 10 varieties in other regions and countries. A total of 570 major AFLP bands were observed, and among them 485 bands (85.1%) were polymorphism. All of the cultivars could be divided with the markers used in this study. Genetic similarities among all taxa ranged from 0.39 (between the two subspecies) to 0.98. Phenetic trees based on genetic similarities (UPGMA, N-J) were similar to known taxonomic relationships. The results showed that the genetic diversity of longan is very high in Guangxi.

Key words: longan, genetic diversity, AFLP

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