园艺学报 ›› 2006, Vol. 33 ›› Issue (6): 1321-1324.

• 研究报告 • 上一篇    下一篇


徐月1, 3;曹庆芹2;冯永庆1;杨凯2;秦岭1;廖康3

  1. (1 北京农学院植物科学技术系, 北京102206; 2 北京农学院生物技术系, 北京102206; 3 新疆农业大学园艺学院, 新疆乌鲁木齐830052)
  • 收稿日期:2006-02-21 修回日期:2006-07-28 出版日期:2006-12-25 发布日期:2006-12-25
  • 通讯作者: 秦岭

AFLP Ana lysis on the Short Ca tkinsMuta tion of Chestnut

Xu Yue1, 3;Cao Qingqin2;Feng Yongqing1;Yang Kai2;Qin Ling1;Liao Kang3   

  1. (1Deparment of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China; 2Deparment of Biology Technology, Beijing University of Agriculture, Beijing 102206, China; 3 Institute of Horticulture, Xinjiang Agricultural University, Urumchi, Xinjiang 830052, China)
  • Received:2006-02-21 Revised:2006-07-28 Online:2006-12-25 Published:2006-12-25
  • Contact: Qin Ling

摘要: 利用AFLP分子标记技术对板栗短雄花序芽变及其母株进行了初步研究, 72种引物组合共产生5 129条清晰谱带, 多态性位点53个, 多态性2.05% , 相似性系数0.9897, 差异片段主要集中于300~900 bp之间。研究表明短雄花序芽变和对照个体之间变异与遗传物质的改变相关, 其中一些可能与雄花序变短的基因有关。

关键词: 板栗, 芽变, 短雄花序, AFLP

Abstract: AFLP was used to compare the short catkins sport and its mother plant in chestnut (Castanea mollissima Bl. ). A total of 72 selective primer pairs amplified 5 129 clear bands, of those 53 were polymorphic loci. The polymorphic rate and Neips association coefficient between them were 2.05% and 0.9897 respectively. The molecular weight range of the polymorphic fragments was mainly 300 - 900 bp. The results indicated that there were some molecular-level differentiation between chestnut and its short catkins sport. Some of the specific segments had been considered as markers of short catkins in chestnut.

Key words: Chestnut, Sport, Short catkin, AFLP