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园艺学报 ›› 2002, Vol. 29 ›› Issue (4): 358-362.

• 研究论文 • 上一篇    下一篇

不同来源红木材料的RAPD 分析及分类学探讨

李晨东1*;唐前瑞2;陈德富1**;夏立新1;陈喜文1;陈友云2   

  1. (1 南开大学生物化学与分子生物学系, 天津300071; 2 湖南农业大学园艺系, 长沙410128)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-08-25 发布日期:2002-08-25

RAPD Analysis and Taxology Discussion on Loropetalum chinense var. rubrumMaterials from Different Fountainhead Areas

Li Chendong1, Tang Qianrui2, Chen Defu1, Xia Lixin1, Chen Xiwen1, and Chen Youyun2   

  1. (1Department of Biochemistry and Molecular Biology , Nankai University , Tianjin 300071, China; 2Department of Horticulture, Hunan Agricultural University , Changsha 410128, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-08-25 Published:2002-08-25

摘要: 以2 个生态型木材料为对照, 对在各原产地收集到的14 个红木材料进行55 个随机引物的RAPD 反应, 共产生632 条稳定的扩增带, 其中427 条具有多态性。按扩增带的有无列出二元数据矩阵,利用NTSYS􀀁pc 计算Nei 氏相似系数及遗传距离, 利用SAS 软件进行主成分分析。结果表明: 红木是木的变种, 供试的14 个红木材料分属4 类, 其突变途径可能是一个复杂的过程, 存在着木不同生态型间、木与红木之间, 红木之间的相互作用。

关键词: 红木, RAPD, 遗传距离, 聚类分析

Abstract: Contrasted with 2 different Loropetalum chinense ecotypes, fourteen Lorop etalum chinense var. rubrum materials representing almost all the types could be were analyzed by RAPD in this paper. Fifty􀀁five random primers produced 632 steady amplified bands in total, among them 427 bands were polymorphic. Strong and clear bands were scored for their presence or absence in a binary data matrix. Nei's similarity coeff icients and genet ic
distances were calculated by NTSYS􀀁pc software, and principal components analysis was done by SAS software. The result demonstrated that L . chinense var. rubrum is a mutant of L . chinense on molecular level and 14 L.
chinense
var. rubrum materials were classified into 4 groups. The mutant pathway of 4 groups is possibly a complicated process, including interactions between different ecotypes L . chinense, interactions between L.
chinense
and L . chinense var. rubrum, and ineractions between different L . chinense var. rubrum.

Key words: Loropetalum chinense var. rubrum, RAPD, Genetic distances, Cluster analysis

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