切花菊耐低钾特性的品种差异及转录组分析

doi:10.16420/j.issn.0513-353x.2024-0874

园艺学报 ›› 2026, Vol. 53 ›› Issue (1): 110-130.doi: 10.16420/j.issn.0513-353x.2024-0874

• 遗传育种·种质资源·分子生物学 • 上一篇下一篇

切花菊耐低钾特性的品种差异及转录组分析

杨可鑫¹^,³^,^*, 唐超越¹^,²^,^*, 杨树华¹, 贾瑞冬¹, 寇亚平¹, 葛红¹^,^**(), 赵鑫¹^,^**()

¹ 中国农业科学院蔬菜花卉研究所，蔬菜生物育种全国重点实验室，农业农村部花卉生物学与种质创制重点实验室（北方），北京 100081
² 三亚中国农业科学院国家南繁研究院，海南三亚 572024
³ 宽城满族自治县林业和草原局，河北承德 067600

收稿日期:2025-08-18 修回日期:2025-12-23 出版日期:2026-01-25 发布日期:2026-01-26
通讯作者:
^**E-mail：zhaoxin01@caas.cn，
gehong@caas.cn
作者简介:
^*共同第一作者
基金资助:
国家重点研发计划课题(2023YFD1200105); 中国农业科学院北方农牧业技术创新中心项目(2022RC-产研院-3)

Varietal Differences and Transcriptome Analyses of Low Potassium Tolerance in Cut Chrysanthemum Cultivars

YANG Kexin¹^,³, TANG Chaoyue¹^,², YANG Shuhua¹, JIA Ruidong¹, KOU Yaping¹, GE Hong¹^,^**(), ZHAO Xin¹^,^**()

¹ State Key Laboratory of Vegetable Biobreeding， Key Laboratory of Biology and Genetic Improvement of Flower Crops（North China），Ministry of Agriculture and Rural Affairs，Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences， Beijing 100081， China
² Sanya National Academy of Southern propagation， Chinese Academy of Agricultural Sciences，Sanya， Hainan 572024， China
³ China Forestry and Grassland Bureau of Kuancheng Manzu Autonomous County， Chengde， Hebei 067600， China

Received:2025-08-18 Revised:2025-12-23 Published:2026-01-25 Online:2026-01-26

摘要/Abstract

摘要：

通过测定4个耐低钾特性显著不同的切花菊（Chrysanthemum × morifolium）品种的苗期表型和养分指标，筛出钾高效利用基因型‘芒果’和钾低效利用基因型‘摩摩卡’，并对两个品种进行低钾处理转录组分析。结果发现，‘芒果’的相对地下鲜、干质量显著高于其他品种，耐低钾综合指数最高，‘摩摩卡’相对性状均为最低值，地上部表型受低钾胁迫影响较大。转录组分析显示，低钾处理下‘芒果’和‘摩摩卡’的叶部差异基因多上调表达，根部差异基因多数下调表达。KEGG分析显示，两品种在低钾胁迫下根部组间独特富集的通路有氨酰基-tRNA生物合成等5个，叶部组间独特富集有植物MAPK信号通路等4个。鉴定出7个钾通道蛋白和7个钾转运蛋白，其中4个AKT基因在‘芒果’和‘摩摩卡’呈现显著不同的表达模式；还鉴定出两个品种在其他营养元素转运、活性氧、植物激素等相关基因的差异表达。综上所述，耐低钾品种‘芒果’可能通过调节自身钾离子通道活性以及多种激素合成和酶活性来增加其对低钾环境的抗性。

关键词: 切花菊, 低钾处理, 钾利用效率, 钾离子通道, 转录组

Abstract:

The seedling phenotypes and nutrient indexes of four cut chrysanthemum cultivars with significantly different low potassium tolerance characteristics were determined，screened out the potassium utilization efficiency genotype‘Mangguo’and the potassium utilization inefficiency genotype‘Momoka’，and performed low potassium-treated transcriptome analyses on two cultivars. The results showed that‘Mangguo’had significantly higher relative total root length，relative stem diameter，and underground fresh weight compared to the other three cultivars，with the highest comprehensive index of low potassium tolerance. In contrast，‘Momoka’exhibited the lowest relative traits under low potassium treatment，and the aboveground phenotypes were more affected by low potassium stress. Transcriptome analysis showed that leaf differential genes were mostly up-regulated and root differential genes were mostly down-regulated in‘Mangguo’and‘Momoka’under low potassium treatment. According to KEGG analysis，the pathways uniquely enriched between the root groups of the two cultivars under low potassium stress included five pathways such as aminoacyl-tRNA biosynthesis，and four pathways such as MAPK signaling pathway-plant were uniquely enriched between the leaf groups. Seven potassium channel proteins and seven potassium transporter proteins were identified，of which four AKT genes showed significantly different expression patterns in‘Mangguo’and‘Momoka’. Differential expression of other genes related to nutrient transport，reactive oxygen species，and phytohormones in the two cultivars was also identified in this experiment. In summary，the low potassium tolerant cultivar‘Mangguo’can increase its resistance under low potassium treatment by regulating its own potassium channel activity as well as a cultivar of hormone syntheses and enzyme activities.

Key words: cut chrysanthemum, low potassium treatment, potassium utilization efficiency, potassium channel, transcriptome

杨可鑫, 唐超越, 杨树华, 贾瑞冬, 寇亚平, 葛红, 赵鑫. 切花菊耐低钾特性的品种差异及转录组分析[J]. 园艺学报, 2026, 53(1): 110-130.

YANG Kexin, TANG Chaoyue, YANG Shuhua, JIA Ruidong, KOU Yaping, GE Hong, ZHAO Xin. Varietal Differences and Transcriptome Analyses of Low Potassium Tolerance in Cut Chrysanthemum Cultivars[J]. Acta Horticulturae Sinica, 2026, 53(1): 110-130.

图/表 15

参考文献 69

[1]	Abdoulaye S, Djibril S, Abdala G D. 2023. Potassium sources,microorganisms and plant nutrition:challenges and future research directions. Pedosphere, 33 (1):105-115. doi: 10.1016/j.pedsph.2022.06.025 URL
[2]	Anderson J A, Huprikar S S, Kochian L V, Lucas W J, Gaber R F. 1992. Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences of the United States of America, 89 (9):3736-3740.
[3]	Buschmann P H, Vaidyanathan R, Gassmann W, Schroeder J I. 2000. Enhancement of Na⁺ uptake currents,time-dependent inward-rectifying K⁺ channel currents,and K⁺ channel transcripts by K⁺ starvation in wheat root cells. Plant Physiology, 122 (4):1387-1397. doi: 10.1104/pp.122.4.1387 pmid: 10759535
[4]	Cakmak I. 2005. The role of potassium in alleviating detrimental effects of abiotic stresses in plants. Journal of Plant Nutrition and Soil Science, 168 (4):521-530.
[5]	Chang C, Li C, Li C Y, Kang X Y, Zou Y J, Ma F W. 2014. Differences in the efficiency of potassium(K)uptake and use in five apple rootstock genotypes. Journal of Integrative Agriculture, 13 (9):1934-1942. doi: 10.1016/S2095-3119(14)60839-X URL
[6]	Chen Guang, Gao Zhenyu, Xu Guohua. 2017. Adaption of plants to potassium deficiency and strategies to improve potassium use efficiency. Chinese Bulletin of Botany, 52 (1):89-101. (in Chinese)
	陈光, 高振宇, 徐国华. 2017. 植物响应缺钾胁迫的机制及提高钾利用效率的策略. 植物学报, 52 (1):89-101. doi: 10.11983/CBB16231
[7]	Deng Z P, Yang J, Chen Y Y, Han H H, Liu X, Yi X P, Wang J C, Yu C W. 2021. Screening high potassium efficiency potato genotypes and physiological responses at different potassium levels. Notulae Botanicae Horti Agrobotanici Cluj-nopaca, 49 (1):12190.
[8]	Department of crop production(Department of Agrochemical Management),ministry of agriculture and rural affairs. 2011. Determination of nitrogen, phosphorus and potassium in plants:NY/T 2017-2011. Beijing:Standards Press of China:1-5. (in Chinese)
	农业农村部种植业管理司. 2011. 植物中氮、磷、钾的测定:NY/T 2017-2011. 北京:中国标准出版社:1-5.
[9]	Department of crop production(Department of Agrochemical Management),ministry of agriculture and rural affairs. 2014. Soil testing-Part 7:method for determination of available phosphorus in soil: NY/T 1121. 7-2014. Beijing:Standards Press of China:1-4. (in Chinese)
	农业农村部种植业管理司. 2014. 土壤检测第七部分:土壤有效磷的测定:NY/T 1121. 7-2014. 北京:中国标准出版社:1-4.
[10]	Du Q, Zhao X H, Xia L, Jiang C J, Wang X G, Han Y, Wang J, Yu H Q. 2017. Effects of potassium deficiency on photosynthesis,chloroplast ultrastructure,ROS,and antioxidant activities in maize(Zea mays L.). Journal of Integrative Agriculture, 18 (2):395-406. doi: 10.1016/S2095-3119(18)61953-7 URL
[11]	Fan M, Huang Y, Zhong Y Q, Kong Q S, Xie J J, Niu M L, Xu Y, Bie Z L. 2014. Comparative transcriptome profiling of potassium starvation responsiveness in two contrasting watermelon genotypes. Planta, 239 (2):397-410. doi: 10.1007/s00425-013-1976-z pmid: 24185372
[12]	Fang X Y, Yang Y R, Zhao Z G, Zhou Y, Liao Y, Guan Z Y, Chen S M, Fang W M, Chen F D, Zhao S. 2023. Optimum nitrogen,phosphorous,and potassium fertilizer application increased chrysanthemum growth and quality by reinforcing the soil microbial community and nutrient cycling function. Plants(Basel), 12 (23):4062.
[13]	Fu Jianxin. 2014. Molecular mechanism of flower transition induced by short-day in Chrysanthemum lavandulifolium[Ph. D. Dissertation]. Beijing: Beijing Forestry University. (in Chinese)
	付建新. 2014. 短日照诱导甘菊成花转变的分子机理[博士论文]. 北京: 北京林业大学.
[14]	Hao Yanshu, Jiang Cuncang, Wang Xiaoli, Xia Ying, Chen Fang. 2011. Differences of potassium efficiency characteristics and root morphology between two cotton genotypes. Acta Agronomica Sinica, 37 (11):2094-2098. (in Chinese) doi: 10.3724/SP.J.1006.2011.02094
	郝艳淑, 姜存仓, 王晓丽, 夏颖, 陈防. 2011. 不同棉花基因型钾效率特征及其根系形态的差异. 作物学报, 37 (11):2094-2098. doi: 10.3724/SP.J.1006.2011.02094
[15]	Hou Jing, Sheng Jiandong, Li Xueni, Chen Bolang. 2008. Screening of cotton varieties with high potassium efficiency at seedling stage. Cotton Science,(2):158-161. (in Chinese)
	侯静, 盛建东, 李雪妮, 陈波浪. 2008. 棉花苗期钾营养高效品种筛选. 棉花学报,(2):158-161. doi: 10.11963/cs080216
[16]	Hu W, Di Q, Zhang J, Liu J, Shi X J. 2020. Response of grafting tobacco to low potassium stress. BMC Plant Biology, 20 (1):286. doi: 10.1186/s12870-020-02481-6 pmid: 32571243
[17]	Jabnoune M, Espeout S, Mieulet D, Fizames C, Verdeil J L, Conejero G, Rodriguez-navarro A, Sentenac H, Guiderdoni E, Abdelly C, Very A A. 2009. Diversity in expression patterns and functional properties in the rice HKT transporter family. Plant Physiology, 150 (4):1955-1971. doi: 10.1104/pp.109.138008 pmid: 19482918
[18]	Jiang Cuncang, Gao Xiangzhao, Wang Yunhua, Lu Jianwei, Xu Fangsen. 2006. Response of difference potassium efficiency cotton genotypes to potassium deficiency. Cotton Science, 18 (2):109-114. (in Chinese)
	姜存仓, 高祥照, 王运华, 鲁剑巍, 徐芳森. 2006. 不同钾效率棉花基因型对低钾胁迫的反应. 棉花学报, 18 (2):109-114.
[19]	Jiang K F, Peng S, Yin Z M, Li X H, Xie L, Shen M C, Li D H, Gao J S. 2024. Effects of N,P,K nutrition levels on the growth,flowering attributes and functional components in Chrysanthemum morifolium. Horticulturae, 10 (3):226. doi: 10.3390/horticulturae10030226 URL
[20]	Johansson I, Wulfetange K, Poree F, Michard E, Gajdanowicz P, Lacombe B, Sentenac H, Thibaud J B, Mueller-roeber B, Blatt M R, Dreyer I. 2006. External K⁺ modulates the activity of the Arabidopsis potassium channel SKOR via an unusual mechanism. Plant Journal, 46 (2):269-281. doi: 10.1111/j.1365-313X.2006.02690.x pmid: 16623889
[21]	Jungk A. 2001. Root hairs and the acquisition of plant nutrients from soil. Journal of Plant Nutrition and Soil Science, 164 (2):121-129. doi: 10.1002/(ISSN)1522-2624 URL
[22]	Karthika K, Richard B, Ma Q F. 2014. Wheat responses to sodium vary with potassium use efficiency of cultivars. Frontiers in Plant Science,(2):5631.
[23]	Kim M J, Ciani S, Schachtman D P. 2010. A peroxidase contributes to ROS production during arabidopsis root response to potassium deficiency. Molecular Plant, 3 (2):420-427. doi: 10.1093/mp/ssp121 pmid: 20139158
[24]	Kumar P, Kumar T, Singh S, Tuteja N, Prasad R, Singh J. 2020. Potassium:a key modulator for cell homeostasis. Journal of Biotechnology,324:198-210.
[25]	Li H, Yu M, Du X Q, Wang Z F, Wu W H, Quintero F J, Jin X H, Li H D, Wang Y. 2017. NRT1. 5/NPF7. 3. Functions as a proton-coupled H⁺/K⁺antiporter for K⁺loading into the xylem in Arabidopsis. Plant Cell, 29 (8):2016-2026. doi: 10.1105/tpc.16.00972 URL
[26]	Li Jingwei, Jiang Chao, Wang Xiaoguang, Song Changhai, Wang Yingqi, Li Li, Song Yingqi, Wang Lijun. 2025. Comprehensive evaluation of agronomic traits in different potato germplasm resources based on principal component analysis and membership function method. China Cucurbits and Vegetables, 38 (9):84-91. (in Chinese)
	李景伟, 姜超, 王小光, 宋昌海, 王英琪, 李莉, 宋瑛琪, 王丽君. 2025. 基于主成分分析和隶属函数法对不同马铃薯种质资源农艺性状的综合评价. 中国瓜菜, 38 (9):84-91.
[27]	Li L Q, Li J, Chen Y, Lu Y F, Lu L M. 2016. De novo transcriptome analysis of tobacco seedlings and identification of the early response gene network under low-potassium stress. Genetics and Molecular Research, 15 (3):1-13.
[28]	Li S Y, Yao X Z, Zhang B H, Tang H, Lu L T. 2023. Genome-wide characterization of the U-box gene in Camellia sinensis and functional analysis in transgenic tobacco under abiotic stresses. Gene,865:147301.
[29]	Li Tingxuan, Ma Guorui. 2004. Physiological and morphological characteristics of roots in grain amaranth genotypes enrichment in potassium. Acta Agronomica Sinica,(11):1145-1151. (in Chinese)
	李廷轩, 马国瑞. 2004. 籽粒苋富钾基因型的根系形态和生理特性. 作物学报,(11):1145-1151.
[30]	Liu C K, Tu B J, Wang X, Jin J, Li Y S, Zang Q Y, Liu X B, Ma B L. 2019. Potassium translocation combined with specific root uptake is responsible for the high potassium efficiency in vegetable soybean. Crop & Pasture Science, 70 (6):516-525.
[31]	Liu C K, Wang X, Tu B J, Li Y S, Liu X B, Zhang Q Y, Herbert S J. 2020. Dry matter partitioning and K distribution of vegetable soybean genotypes with higher potassium efficiency. Archives of Agronomy and Soil Science, 66 (5):717-729. doi: 10.1080/03650340.2019.1638508 URL
[32]	Love M I, Huber W, Ander S S. 2014. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biology, 15 (12):550. doi: 10.1186/s13059-014-0550-8 URL
[33]	Ma T L, Wu W H, Wang Y. 2012. Transcriptome analysis of rice root responses to potassium deficiency. BMC Plant Biology,12:161.
[34]	Ministry of Agriculture and Rural Affairs. 2004. Determination of exchangeable potassium and non-exchangeable potassium content in soil: NY/T 889-2004. Beijing:Standards Press of China:1-3. (in Chinese)
	农业农村部. 2004. 土壤速效钾和缓效钾含量的测定:NY/T 889-2004. 北京:中国标准出版社:1-3.
[35]	Nam Y J, Tran L S, Kojima M, Sakakibara H, Nishiyama R, Shin R. 2012. Regulatory roles of cytokinins and cytokinin signaling in response to potassium deficiency in Arabidopsis. PLoS ONE, 7 (10):e47797. doi: 10.1371/journal.pone.0047797 URL
[36]	Nieves-cordones M, Martinez-cordero M A, Martinez V, Rubio F. 2007. An NH4⁺-sensitive component dominates high-affinity K⁺ uptake in tomato plants. Plant Science, 172 (2):273-280. doi: 10.1016/j.plantsci.2006.09.003 URL
[37]	Pettigrew W T. 2008. Potassium influences on yield and quality production for maize,wheat,soybean and cotton. Physiologia Plantarum, 133 (4):670-681. doi: 10.1111/ppl.2008.133.issue-4 URL
[38]	Ragel P, Raddatz N, Leidi E O, Quintero F J, Pardo J M. 2019. Regulation of K⁺ nutrition in plants. Frontiers in Plant Science,10:281.
[39]	Research institute of forestry chinese academy of forestry. 1999. Determination of hydrolyzable nitrogen in forest soil:LY/T 1299-1999. Beijing:Standards Press of China:78-80. (in Chinese)
	中国林业科学研究院林业研究所. 1999. 森林土壤水解性氮的测定:LY/T 1299-1999. 北京:中国标准出版社:78-80.
[40]	Robinson M D, McCarthy D J, Smyth G K. 2010. edgeR:a bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics(Oxford,England), 26 (1):139-140.
[41]	Ruan L, Cheng H, Ludewig U, Li J W, Chang S X. 2022. Root foraging strategy improves the adaptability of tea plants(Camellia sinensis L. ) to soil potassium heterogeneity. International Joural of Molecular Sciences, 23 (15):8585.
[42]	Schachtman D P. 2015. The role of ethylene in plant responses to K⁺ deficiency. Frontiers in Plant Science,6:1153.
[43]	Shin R, Schachtman D P. 2004. Hydrogen peroxide mediates plant root cell response to nutrient deprivation. Proceedings of the National Academy of Sciences of the United States of America, 101 (23):8827-8832.
[44]	Song A P, Su J S, Wang H B, Zhang Z R, Zhang X T, Van d P Y, Chen F, Fang W M, Guan Z Y, Zhang F, Wang Z X, Wang L K, Ding B Q, Zhao S, Ding L, Liu Y, Zhou L J, He J, Jia D W, Zhang J L, Chen C W, Yu Z Y, Sun D J, Jiang J F, Chen S M, Chen F D. 2023. Analyses of a chromosome-scale genome assembly reveal the origin and evolution of cultivated chrysanthemum. Nature Communications, 14 (1):2021. doi: 10.1038/s41467-023-37730-3 pmid: 37037808
[45]	Sun T T, Zhang J K, Zhang Q, Li X L, Li M J, Yang Y Z, Zhou J, Wei Q P, Zhou B B. 2023. Transcriptional and metabolic responses of apple to different potassium environments. Front in Planta Science,14:1131708.
[46]	Sustr M, Soukup A, Tylova E. 2019. Potassium in root growth and development. Plants,8:435.
[47]	Tahir M M, Chen S Y, Ma X Y, Li S H, Zhang X Y, Shao Y, Shalmani A, Zhao C P, Bao L, Zhang D. 2021. Transcriptome analysis reveals the promotive effect of potassium by hormones and sugar signaling pathways during adventitious roots formation in the apple rootstock. Plant Physiol Biochem,165:123-136.
[48]	Wang F, Tan W F, Song W, Yang S T, Qiao S. 2022. Transcriptome analysis of sweet potato responses to potassium deficiency. BMC Genomics, 23 (1):655. doi: 10.1186/s12864-022-08870-5 pmid: 36109727
[49]	Wang X, Luo J F, Liu R, Liu X, Jiang J. 2023. Gibberellins regulate root growth by antagonizing the jasmonate pathway in tomato plants in response to potassium deficiency. Scientia Horticulturae,309:5.
[50]	Wang X P, Hao L, Zhu B P, Jiang Z H. 2018. Plant calcium signaling in response to potassium deficiency. International Journal of Molecular Sciences, 19 (11):3456. doi: 10.3390/ijms19113456 URL
[51]	Wang Xiao, Xia Ying, Wang Li, Chen Fang. 2012. Research on the response of different potassium efficiency cotton genotypes to water and low potassium stress. Chinese Agricultural Science Bulletin, 28 (9):60-65. (in Chinese) doi: 10.11924/j.issn.1000-6850.2011-2625
	汪霄, 夏颖, 王利, 陈防. 2012. 棉花不同钾效率基因型对水分和低钾胁迫的响应研究. 中国农学通报, 28 (9):60-65.
[52]	Wang Y, Chen Y F, Wu W H. 2021a. Potassium and phosphorus transport and signaling in plants. Journal of Integrative Plant Biology,63:34-52.
[53]	Wang Y, Wu W H. 2017. Regulation of potassium transport and signaling in plants. Current Opinion in Plant Biology,39:123-128.
[54]	Wang Y F, Dai X Y, Xu G Q, Dai Z Y, Chen P Y, Zhang T J, Zhang H F. 2021b. The Ca²⁺-CaM signaling pathway mediates potassium uptake by regulating reactive oxygen species homeostasis in tobacco roots under low-K⁺stress. Frontires in Plant Science,12:658509.
[55]	Wang Yong, Li Tingxuan, Chen Guangdeng, Yang Huan, Meng Lin. 2017. Study on K absorption and physiological and biochemical characteristics of different K-efficiency tobacco genotypes. Chinese Tobacco Science, 38 (5):56-61. (in Chinese)
	王勇, 李廷轩, 陈光登, 杨欢, 孟霖. 2017. 不同钾基因型烟草钾吸收和生理生化特性研究. 中国烟草科学, 38 (5):56-61.
[56]	Wani A H, Mir S H, Kumar S, Malik M A, Tyub S, Rashid I. 2022. Silicon enroute-from loam to leaf. Plant Growth Regulation, 99 (3):465-476. doi: 10.1007/s10725-022-00931-9
[57]	White P J. 2013. Improving potassium acquisition and utilisation by crop plants. Journal of Plant Nutrition and Soil Science,176:305-316.
[58]	Wu Jintao, Zhang Xizhou, Li Tingxuan, Yu Haiying. 2010. Study on K absorption and its physiological and biochemical characteristics of K-efficient barley genotype. Journal of Plant Nutrition and Fertilizers, 16 (4):906-912. (in Chinese)
	吴金涛, 张锡洲, 李廷轩, 余海英. 2010. 大麦钾高效基因型钾吸收和生理生化特性研究. 植物营养与肥料学报, 16 (4):906-912.
[59]	Xu J, Tian X L, Eneji A E, Li Z H. 2014. Functional characterization of GhAKT1,a novel shaker-like K⁺ channel gene involved in K⁺ uptake from cotton(Gossypium hirsutum). Gene, 545 (1):61-71. doi: 10.1016/j.gene.2014.05.006 URL
[60]	Xu Shunli, Fang Weimin, Guan Zhiyong, Jiang Jiafu, Chen Sumei, Liao Yuan, Chen Fadi. 2013. Screening cut chrysanthemum varieties in low potassium tolerant and patience physiology in seedling. Acta Horticulturae Sinica, 40 (12):2463-2471. (in Chinese)
	徐顺莉, 房伟民, 管志勇, 蒋甲福, 陈素梅, 廖园, 陈发棣. 2013. 耐低钾切花菊品种筛选及其苗期耐性生理研究. 园艺学报, 40 (12):2463-2471.
[61]	Xu X X, Liu G Y, Liu J Q, Lyu M X, Wang F, Xing Y, Meng H, Li M, Jiang Y, Tian G, Zhu Z L, Jiang Y M, Ge S F. 2024. Potassium alleviated high nitrogen-induced apple growth inhibition by regulating photosynthetic nitrogen allocation and enhancing nitrogen utilization capacity. Horticultural Plant Journal, 10 (1):1-14. doi: 10.1016/j.hpj.2023.04.003 URL
[62]	Yang D D, Li F J, Yi F, Egrinya A E, Tian X L, Li Z H. 2021. Transcriptome analysis unravels key factors involved in response to potassium deficiency and feedback regulation of K⁺ uptake in cotton roots. International Journal of Molecular Sciences, 22 (6):3133. doi: 10.3390/ijms22063133 URL
[63]	Yang Kexin. 2023. Physiological response and molecular mechanism of cut chrysanthemum under reduced fertilization and low potassium treatment[M. D. Disseration]. Beijing:Chinese Academy of Agricultural Sciences. (in Chinese)
	杨可鑫. 2023. 切花菊减量施肥及低钾处理下的生理响应和分子机制[硕士论文]. 北京: 中国农业科学院.
[64]	Yang Kexin, Zhao Xin, Ge Hong, Yang Shuhua, Jia Ruidong, Kou Yaping. 2022. nutrition and fertilizer efficient utilization technology of cut chrysanthemum:research progress. Chinese Agricultural Science Bulletin, 38 (34):52-58. (in Chinese) doi: 10.11924/j.issn.1000-6850.casb2021-1206
	杨可鑫, 赵鑫, 葛红, 杨树华, 贾瑞冬, 寇亚平. 2022. 切花菊营养与肥料高效利用技术研究进展. 中国农学通报, 38 (34):52-58. doi: 10.11924/j.issn.1000-6850.casb2021-1206
[65]	Zeng J B, He X Y, Wu D Z, Zhu B, Cai S G, Umme A N, Zahra J, Zhang G P. 2014. Comparative transcriptome profiling of two Tibetan wild barley genotypes in responses to low potassium. PLoS ONE, 9 (6):e100567. doi: 10.1371/journal.pone.0100567 URL
[66]	Zhang M L, Hu Y Y, Han W, Chen J, Lai J S, Wang Y. 2023a. Potassium nutrition of maize:uptake,transport,utilization,and role in stress tolerance. The Crop Journal, 11 (4):1048-1058. doi: 10.1016/j.cj.2023.02.009 URL
[67]	Zhang M X, Qin S Q, Yan J P, Li L, Xu M Z, Liu Y R, Zhang W J. 2023b. Genome-wide identification and analysis of TCP family genes in Medicago sativa reveal their critical roles in Na⁺/K⁺ homeostasis. BMC Plant Biology, 23 (1):301. doi: 10.1186/s12870-023-04318-4
[68]	Zhang X C, Wu H H, Chen J G, Chen L M, Chang N, Ge G F, Wang X C. 2020. Higher ROS scavenging ability and plasma membrane H⁺-ATPase activity are associated with potassium retention in drought tolerant tea plants. Journal of Plant Nutrition and Soil Science, 183 (4):406-415. doi: 10.1002/jpln.v183.4 URL
[69]	Zhao X M, Liu Y, Liu X, Jiang J. 2018. Comparative transcriptome profiling of two tomato genotypes in response to potassium-deficiency stress. International Journal of Molecular Sciences, 19 (8):2402. doi: 10.3390/ijms19082402 URL

0.1 mmol · L^-1 K⁺		2.5 mmol · L^-1 K⁺
营养液成分 Nutrient solution composition	浓度/（mg · L^-1） Concentration	营养液成分 Nutrient solution composition	浓度/（mg · L^-1） Concentration
Ca（NO₃）2 ∙ 4H₂O	945.000	Ca（NO₃）2 ∙ 4H₂O	945.000
KNO₃	10.000	KNO₃	253.000
NH₄⁺	236.000	NH₄⁺	140.000
NH₄H₂PO₄	115.000	NH₄H₂PO₄	120.000
MgSO₄	241.000	MgSO₄	241.000
H₃BO₃	6.200	H₃BO₃	6.200
CoCl₂∙ 5H₂O	0.025	CoCl₂∙ 5H₂O	0.025
CuSO₄∙ 5H₂O	0.025	CuSO₄∙ 5H₂O	0.025
FeNaEDTA	36.700	FeNaEDTA	36.700
MnSO₄∙ H₂O	16.900	MnSO₄∙ H₂O	16.900
Na₂MoO₄∙ 2H₂O	0.250	Na₂MoO₄∙ 2H₂O	0.250
KI	0.830	KI	0.830
ZnSO₄∙ 7H₂O	8.600	ZnSO₄∙ 7H₂O	8.600

0.1 mmol · L^-1 K⁺		2.5 mmol · L^-1 K⁺
营养液成分 Nutrient solution composition	浓度/（mg · L^-1） Concentration	营养液成分 Nutrient solution composition	浓度/（mg · L^-1） Concentration
Ca（NO₃）2 ∙ 4H₂O	945.000	Ca（NO₃）2 ∙ 4H₂O	945.000
KNO₃	10.000	KNO₃	253.000
NH₄⁺	236.000	NH₄⁺	140.000
NH₄H₂PO₄	115.000	NH₄H₂PO₄	120.000
MgSO₄	241.000	MgSO₄	241.000
H₃BO₃	6.200	H₃BO₃	6.200
CoCl₂∙ 5H₂O	0.025	CoCl₂∙ 5H₂O	0.025
CuSO₄∙ 5H₂O	0.025	CuSO₄∙ 5H₂O	0.025
FeNaEDTA	36.700	FeNaEDTA	36.700
MnSO₄∙ H₂O	16.900	MnSO₄∙ H₂O	16.900
Na₂MoO₄∙ 2H₂O	0.250	Na₂MoO₄∙ 2H₂O	0.250
KI	0.830	KI	0.830
ZnSO₄∙ 7H₂O	8.600	ZnSO₄∙ 7H₂O	8.600

性状Trait	低钾胁迫敏感度不同品种 Varieties with different sensitivities to potassium deficiency stress
性状Trait	摩摩卡Momoka	紫妍Ziyan	凯文白Kaiwenbai	芒果Mangguo
相对株高Relative height	0.70 ± 0.012 b	0.83 ± 0.057 a	0.93 ± 0.030 a	0.88 ± 0.008 a
相对茎粗Relative stem diameter	0.86 ± 0.053 b	0.93 ± 0.021 a	0.93 ± 0.004 a	1.00 ± 0.090 a
相对总根长Relative root length	0.70 ± 0.084 a	0.70 ± 0.059 a	0.74 ± 0.019 a	0.85 ± 0.072 a
相对根平均直径Relative mean root diameter	0.65 ± 0.057 a	0.55 ± 0.012 a	0.58 ± 0.029 a	0.80 ± 0.004 a
相对地上鲜质量Relative aboveground fresh weight	0.54 ± 0.019 c	0.72 ± 0.142 b	0.86 ± 0.073 a	0.83 ± 0.007 ab
相对地下鲜质量Relative underground fresh weight	0.55 ± 0.057 b	0.47 ± 0.035 b	0.67 ± 0.029 b	1.12 ± 0.004 a
相对地上干质量Relative aboveground dry weight	0.69 ± 0.049 c	0.95 ± 0.181 b	1.16 ± 0.131 a	0.98 ± 0.020 b
相对地下干质量Relative underground dry weight	0.54 ± 0.054 c	0.75 ± 0.093 bc	0.80 ± 0.025 ab	0.92 ± 0.018 a

性状Trait	低钾胁迫敏感度不同品种 Varieties with different sensitivities to potassium deficiency stress
性状Trait	摩摩卡Momoka	紫妍Ziyan	凯文白Kaiwenbai	芒果Mangguo
相对株高Relative height	0.70 ± 0.012 b	0.83 ± 0.057 a	0.93 ± 0.030 a	0.88 ± 0.008 a
相对茎粗Relative stem diameter	0.86 ± 0.053 b	0.93 ± 0.021 a	0.93 ± 0.004 a	1.00 ± 0.090 a
相对总根长Relative root length	0.70 ± 0.084 a	0.70 ± 0.059 a	0.74 ± 0.019 a	0.85 ± 0.072 a
相对根平均直径Relative mean root diameter	0.65 ± 0.057 a	0.55 ± 0.012 a	0.58 ± 0.029 a	0.80 ± 0.004 a
相对地上鲜质量Relative aboveground fresh weight	0.54 ± 0.019 c	0.72 ± 0.142 b	0.86 ± 0.073 a	0.83 ± 0.007 ab
相对地下鲜质量Relative underground fresh weight	0.55 ± 0.057 b	0.47 ± 0.035 b	0.67 ± 0.029 b	1.12 ± 0.004 a
相对地上干质量Relative aboveground dry weight	0.69 ± 0.049 c	0.95 ± 0.181 b	1.16 ± 0.131 a	0.98 ± 0.020 b
相对地下干质量Relative underground dry weight	0.54 ± 0.054 c	0.75 ± 0.093 bc	0.80 ± 0.025 ab	0.92 ± 0.018 a

性状Trait	摩摩卡 Momoka	芒果 Mangguo
相对地上部钾含量Relative shoot potassium content	0.32 ± 0.01 b	0.41 ± 0 a
相对根部钾含量Relative root potassium content	8.05 ± 0.41 a	1.18 ± 0.01 b
相对地上部钾累积量Relative shoot potassium accumulation	0.22 ± 0.03 b	0.40 ± 0.01 a
相对根部钾累积量Relative root potassium accumulation	4.43 ± 1.26 a	1.08 ± 0.22 b
相对地上部钾素利用率Relative shoot potassium utilization rate	3.14 ± 0.12 a	2.44 ± 0.03 b
相对根部钾素利用率Relative root potassium utilization rate	0.12 ± 0.01 b	0.85 ± 0.01 a

切花菊耐低钾特性的品种差异及转录组分析

Varietal Differences and Transcriptome Analyses of Low Potassium Tolerance in Cut Chrysanthemum Cultivars

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 15

参考文献 69

相关文章 15

编辑推荐

Metrics

本文评价

访问总数:　当日访问总数:　当前在线人数:

编号 ID	差异倍数（log₂fc）Variance multiplier				描述 Description	标志 Symbol
编号 ID	M_HR vs. M_LR	A_HR vs. A_LR	M_HL vs. M_LL	A_HL vs. A_LL	描述 Description	标志 Symbol
Unigene0004899	3.20	2.32	-1.74	-	钾转运蛋白Potassium transporter	HAK5
Unigene0000573	1.11	1.34	-	-	钾通道，电压依赖性，EAG/ELK/ERG，瞬时受体电位通道 Potassium channel，voltage-dependent，EAG/ELK/ERG，Transient receptor potential channel	AKT1
Unigene0083644	-	-1.32	-	1.14	K⁺转运蛋白1 K⁺ transporter 1	AKT1
Unigene0089510	1.09	1.09	-	1.08	钾通道，电压依赖性，EAG/ELK/ERG，瞬时受体电位通道 Potassium channel，voltage-dependent，EAG/ELK/ERG，Transient receptor potential channel	AKT1
Unigene0029619	-9.60	-14.57	-1.46	4.54	蛋白激酶 Protein kinase	AKT3
Unigene0088233	-9.91	-12.02	-	-	蛋白激酶 Protein kinase	AKT1
Unigene0061927	4.61	2.72	-	-	钾离子转运蛋白HKT6 Cation transporter HKT6	HKT1
Unigene0067717	-1.05	-1.11	-	-	高亲和力 K⁺转运蛋白1 High-affinity K⁺transporter 1	HKT6
Unigene0069625	-	-	1.25	-	双孔钾通道5 Two-pore potassium channel 5	TPK5
Unigene0045437	1.24	-	-1.45	-	EF-手结构域对，钾通道结构域蛋白 EF-hand domain pair，Potassium channel domain protein	TPK1
Unigene0093310	1.57	-	-	-	硫胺素焦磷酸激酶3 Thiamine pyrophosphokinase 3	TPK1
Unigene0005531	-1.01	-1.74	-	-	谷胱甘肽调控的钾外流系统蛋白KefB Glutathione-regulated potassium-efflux system protein KefB	KEA2
Unigene0042129	-13.87	-7.89	-3.33	-3.33	钠钾氢离子反向转运蛋白 Na⁺/K⁺/H⁺ antiporter 9	NHX5
Unigene0080270	1.54	-	-	-	液泡钠氢共转运蛋白 Vacuolar Na⁺/H⁺ antiporter protein	NHX2
Unigene0043899	-13.22	-8.37	-	-	未命名的蛋白质产物 Unnamed protein product	CPA1

[1]	孙东宇, 魏冬, 杨胤延, 胡彩珠, 胡志群, 周东辉, 周碧燕. 莲雾开花对喷施氯吡硫磷的生理响应以及转录组分析[J]. 园艺学报, 2025, 52(7): 1718-1732.
[2]	李雪松, 华蓉, 孙达锋, 岳万松, 张俊波, 李建英, 刘绍雄. 金耳SSR分子标记开发及指纹编码构建[J]. 园艺学报, 2025, 52(7): 1780-1788.
[3]	杨春梅, 于洋, 丁雨格, 夏京, 周玲, 彭磊. 转录—代谢联合分析‘贵妃’杧果腋芽转化花芽中的淀粉与蔗糖代谢途径[J]. 园艺学报, 2025, 52(6): 1412-1426.
[4]	郭新淼, 田露瑶, 曹佳琪, 谢岩, 高妍夏, 孙志超. 白色桑椹颜色缺失分子机制初步探讨[J]. 园艺学报, 2025, 52(6): 1451-1462.
[5]	李姿燕, 陈炜曦, 李子涵, 黎茵, 梁峰铭, 曾祥利, 荐红举, 吕典秋. 基于RNA-Seq筛选调控马铃薯熟性的候选基因[J]. 园艺学报, 2025, 52(6): 1505-1518.
[6]	张悦, 冯一了, 王蓓, 任文静, 姜春昱, 赵新宇, 王彩红, 杨丽梅, 庄木, 吕红豪, 王勇, 张扬勇, 季家磊. 甘蓝叶酸合成代谢的转录组初步分析[J]. 园艺学报, 2025, 52(5): 1301-1316.
[7]	袁娟伟, 贾利, 王涵, 严从生, 张其安, 俞飞飞, 甘德芳, 江海坤. 辣椒种质资源疫病抗性鉴定及抗病基因挖掘[J]. 园艺学报, 2025, 52(4): 857-871.
[8]	唐伶俐, 贺玉花, 王庆涛, 安璐璐, 徐永阳, 张健, 孔维虎, 户克云, 赵光伟. 非呼吸跃变和呼吸跃变型甜瓜成熟果实的激素与转录组分析[J]. 园艺学报, 2025, 52(4): 883-896.
[9]	邵一帆, 朱宝庆, 王童欣, 廖建和, 吴繁花, 杨思怡, 冯建行, 于旭东. 基于激素、转录组和代谢组研究木棉皮刺的遗传调控[J]. 园艺学报, 2025, 52(4): 933-946.
[10]	吝茹雪, 王斐, 张艳杰, 马力, 刘肖烽, 李舒然, 刘亚龙, 王晨, 姜淑苓, 欧春青. 梨矮生相关基因转录组筛选与功能分析[J]. 园艺学报, 2025, 52(3): 545-560.
[11]	赵超胜, 陈梦然, 郑永琪, 李菊, 马培芳, 肖雪梅, 郁继华. 韭菜S-烃基半胱氨酸亚砜合成与分解代谢关键基因筛选[J]. 园艺学报, 2025, 52(12): 3257-3270.
[12]	彭朝凤, 齐帅征, 耿喜宁, 姚鹏强, 谢丽华, 张钰, 陈明辉, 施江, 程世平. 不同倍性‘凤丹’牡丹叶片转录组差异表达分析[J]. 园艺学报, 2025, 52(12): 3288-3302.
[13]	冯克政, 王康, 汪永康, 赵龙龙, 廖娜, 唐小燕, 王文杰, 汪承刚, 袁凌云, 侯金锋, 吴建强, 陈国户. 乌塌菜雄性不育关键基因的鉴定及功能分析[J]. 园艺学报, 2025, 52(11): 2876-2888.
[14]	郑聪慧, 徐振华, 张蔓蔓, 王玉忠, 李向军, 杜克久, 孙然, 李亚. ‘朝阳椿’叶片类黄酮合成的转录组—代谢组联合分析[J]. 园艺学报, 2025, 52(11): 2913-2930.
[15]	樊阿梅, 靳亚鑫, 何龙娇, 李琦, 张燕青, 邵祺, 宋芸, 牛颜冰, 乔永刚. 赤霉素调控连翘异型花柱生长发育的研究[J]. 园艺学报, 2025, 52(11): 3016-3030.

切花菊耐低钾特性的品种差异及转录组分析

Varietal Differences and Transcriptome Analyses of Low Potassium Tolerance in Cut Chrysanthemum Cultivars

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 15

参考文献 69

相关文章 15

编辑推荐

Metrics

本文评价

访问总数: 当日访问总数: 当前在线人数:

访问总数:　当日访问总数:　当前在线人数: