关键词: 莲藕, 甘薯潜隐病毒, 实时荧光定量PCR

Abstract: During the survey of lotus viruses disease，sweet potato latent virus-lotus was found to occur at high frequencies in Jiangsu province, China. And there is a significant sequence difference between SPLV-lotus and sweet potato isolates. In order to detect SPLV more accurately，a specific RT-qPCR assay based on SYBR GreenⅠfluorescent dye was established. A specific primer pair QSPLV- lotus3-F/QSPLV-lotus3-R was designed based on the CP gene of SPLV-lotus，and a standard curve was constructed under the optimized primer concentration and annealing temperature. The results showed that the RT-qPCR assay had higher specificity and was 100 times more sensitive for SPLV detection than conventional RT-PCR. This method can be widely used in batch inspection of field samples and be suitable for the detection and identification of virus-free lotus root seedlings.

Key words: lotus, sweet potato latent virus-lotus, RT-qPCR