园艺学报 ›› 2019, Vol. 46 ›› Issue (3): 590-600.doi: 10.16420/j.issn.0513-353x.2018-0713

• 研究报告 • 上一篇    下一篇

采用胚挽救获得Ogura CMS甘蓝与甘蓝型油菜的种间杂种

姜 娇,张恩慧,李升娟,石汶汶,吴晓凤,郭 佳,宫海军,许忠民*   

  1. (西北农林科技大学园艺学院,陕西杨凌 712100)
  • 出版日期:2019-03-25 发布日期:2019-03-25

Obtained Interspecific Hybrids Between Ogura CMS Lines of Brassica oleracea and Brassica napus Through Embryo Rescue

JIANG Jiao,ZHANG Enhui,LI Shengjuan,SHI Wenwen,WU Xiaofeng,GUO Jia,GONG Haijun,and XU Zhongmin*   

  1. (College of Horticulture,Northwest A & F University,Yangling,Shaanxi 712100,China)
  • Online:2019-03-25 Published:2019-03-25

摘要: 为将甘蓝型油菜细胞质雄性不育系(Ogura CMS)的恢复基因转至甘蓝Ogura CMS材料上,以6份甘蓝Ogura CMS材料为母本,以含恢复基因的甘蓝型油菜为父本,人工杂交结合胚挽救培养,研究取材时期、培养基成分和杂交组合对胚珠成苗的影响,同时对胚挽救培养获得的植株是否为真实F1杂种进行鉴定。结果发现,胚珠培养以剥蕾授粉后16 d取材时胚珠成苗数最多,成苗率为4.56%;培养基以MS + GA 0.1 mg ? L-1 + NAA 0.1 mg ? L-1 + 0.5%水解酪蛋白(CH)+ 0.5%活性炭(AC)成苗效果最好,成苗率高达6.24%;M09CMS × RFO-46组合成苗数最多,成苗率为5.96%。67株胚挽救培养得到的植株经流式细胞仪、SSR分子标记和形态学鉴定,得出65株为真杂种,真杂种率高达97%。

关键词: 甘蓝, 远缘杂交, 胚挽救, 鉴定

Abstract: In order to transfer the Ogura CMS restorer gene of Brassica napus to the Ogura CMS lines of Brassica oleracea,6 Ogura CMS lines of cabbage and the B. napus which contained the restorer gene were used as the female and male parents respectively to obtain the F1 generation through embryo rescue culture and the artificial hybridization technology,and their authenticity was also identified. Investigation was carried out on the factors which could affect ovule seedling formation,including sampling time,medium composition and cross combination. The results showed that the ovular seedling formation efficiency was the highest while using ovules 16 d after pollination,altogether the seedling rate was 4.56%. The medium of MS + GA 0.1 mg ? L-1 + NAA 0.1 mg ? L-1 + 0.5% casein hydrolyzate + 0.5% activated carbon,had the best seedling growing-up with the seedling rate as high as 6.24%. The cross combination of M09CMS × RFO-46 generated the most F1 generation seedlings with the 5.96% seedling rate. Sixty five out of 67 F1 generation seedlings were identified as the true hybrids through flow cytometry,SSR molecular markers and morphological identification,accounting for 97%.

Key words: cabbage, distant hybridization, embryo rescue, identification