园艺学报 ›› 2016, Vol. 43 ›› Issue (11): 2266-2276.doi: 10.16420/j.issn.0513-353x.2016-0440

• 研究报告 • 上一篇    下一篇

二倍体马铃薯栽培品种遗传多样性的SSR 标记分析

宋晓燕1,张春芝2,3,李 颖2,冯爽爽1,2,杨 清1,*,黄三文2,3,*   

  1. 1 南京农业大学生命科学学院,南京 210095;2 中国农业科学院蔬菜花卉研究所,农业部园艺作物生物学与遗传改
    良重点实验室,北京 100081;3 中国农业科学院深圳农业基因组研究所,广东深圳 518120
  • 出版日期:2016-11-25 发布日期:2016-11-25
  • 基金资助:


SSR Analysis of Genetic Diversity Among 192 Diploid Potato Cultivars

  1. 1College of Life Science,Nanjing Agricultural University,Nanjing 210095,China;2Key Laboratory of Biology and
    Genetic Improvement of Horticultural Crops,Ministry of Agriculture,Institute of Vegetables and Flowers,Chinese Academy
    of Agricultural Sciences,Beijing 100081,China;3Agricultural Genomic Institute at Shenzhen,Chinese Academy of
    Agricultural Sciences,Shenzhen,Guangdong 518120,China
  • Online:2016-11-25 Published:2016-11-25


价二倍体马铃薯种质的遗传多样性,挖掘和有效利用优良性状显得非常必要。为了筛选多态性的SSR 标
记,用55 对SSR 引物扩增39 个遗传关系相对较远的二倍体马铃薯材料。选取分布在12 条染色体上的
12 个具有高多态性的SSR 标记评价192 份二倍体马铃薯栽培品种的遗传多样性,共检测到98 个等位位
点,其中97 个为多态性位点;每对SSR 引物扩增出的等位位点为6 ~ 18 个,平均8.2 个。用非加权配对
算术平均法(UPGMA)进行聚类,显示出所有供试材料的遗传关系:12 对SSR 引物可以将192 份材料
中的186 份区分开;这192 份材料被划分为11 个组群,其中第一个组群包含了83.3%的材料。

关键词: 马铃薯, 二倍体栽培品种, SSR 标记, 遗传多样性, DNA 指纹图谱


In potato breeding,it is difficult to improve the traits of interest at the tetraploid level due
to the tetrasomic inheritance. A promising supplement is diploid breeding due to the simple genome. Thus,
it is necessary to assess the genetic diversity of diploid potato germplasm for efficient exploration and
deployment of desirable traits. In this study,we used simple sequence repeat(SSR) markers to evaluate
the genetic diversity of diploid potato cultivars,which can provide a molecular basis for diploid potato
breeding. To screen polymorphic SSR markers,55 pairs of SSR primers were employed to amplify 39
cultivars with relatively distant genetic relationships. Among them,12 SSR markers with high
polymorphism located at 12 chromosomes were chosen to evaluate the genetic diversity of 192 diploid potato cultivars. The primers produced 6 to 18 bands with an average of 8.2 bands per primer. In total,98
bands were amplified from 192 cultivars,97 of which were polymorphic. Cluster analysis using UPGMA
showed the genetic relationships of all accessions tested:One-hundred and eighty-six of the 192 accessions
could be distinguished by only 12 pairs of SSR primers,the 192 diploid cultivars were divided into 11
groups,and 83.3% constituted the first group.

Key words: Solanum tuberosum, diploid potato cultivated species, SSR marker, genetic diversity, DNA fingerprinting