Abstract:

The Rcd1 protein is the major subunit of the CCR4-NOT protein complex and is one of the most conserved proteins found in eukaryotes，and its homologs are expressed in a variety of differentiated tissues. In order to understand the function of Rcd1 protein in response to abiotic stress in potato，the StRcd1 gene was cloned from potato cultivar‘Qingshu 9’. The expression pattern of the gene under simulated drought stress and salt stress was analyzed. The results showed that the full length of StRcd1 was 1 223 bp，the open reading frame was 897 bp，encoding 298 amino acids，and the subcellular cells were located in the nucleus. StRcd1 was highly expressed in potato tubers and responded to drought and salt stress，but the expression patterns in different tissues were different. The expression of the roots was the highest under the salt stress of 12 h and drought stress of 24 h，which were 5.96 times and 9.37 times of the control，respectively. It is speculated that StRcd1 plays an important role in response to drought and salt stress in potato.

Key words: potato, required cell differentiation 1, gene clone；expression analysis