Tomato Double Mutant of Histone Variant Gene was Obtained and its Function Research

doi:10.16420/j.issn.0513-353x.2018-0046

ACTA HORTICULTURAE SINICA ›› 2018, Vol. 45 ›› Issue (6): 1081-1088.doi: 10.16420/j.issn.0513-353x.2018-0046

• Research Papers • Previous Articles Next Articles

Tomato Double Mutant of Histone Variant Gene was Obtained and its Function Research

YANG Xuedong*，TIAN Shoubo*，ZHU Weimin，LU Panling，WANG Hong，WANG Ying，ZHANG Hui**，and ZHU Longying**

The Protected Horticulture Institute，Shanghai Academy of Agricultural Sciences，Shanghai Key Laboratory of Protected Horticulture Technology，Shanghai 201403，China

Online:2018-06-25 Published:2018-06-25

Abstract

Abstract: Using tomato inbred line 1 479 as the material，a double mutant of H2A.Z gene of histone variant hta11hta9 was obtained by gene editing technique. Sequencing analysis showed that the tomato H2A.Z gene was knocked out. The results of germination experiment showed that H2A.Z double mutant hta11hta9 had a serious effect on seed germination. The growth of H2A.Z double mutant hta11hta9 was obviously slower than wild type under the relative low temperature of 17 ℃.

Key words: tomato, gene editing, histone variant

CLC Number:

S 641.2

YANG Xuedong*，TIAN Shoubo*，ZHU Weimin，LU Panling，WANG Hong，WANG Ying，ZHANG Hui**，and ZHU Longying**. Tomato Double Mutant of Histone Variant Gene was Obtained and its Function Research[J]. ACTA HORTICULTURAE SINICA, 2018, 45(6): 1081-1088.

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Tomato Double Mutant of Histone Variant Gene was Obtained and its Function Research

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