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ACTA HORTICULTURAE SINICA ›› 2020, Vol. 47 ›› Issue (8): 1521-1529.doi: 10.16420/j.issn.0513-353x.2019-0935

• Research Papers • Previous Articles     Next Articles

Cloning and Expression Analysis of Lb_PPR1 Gene in Different Lycium barbarum Fertility Varieties

GUAN Cuiping,YANG Yajun,and SHI Jing*   

  1. College of Life Science,Ningxia University;Western Biological Resources Protection and Utilization Lab of National Education Ministry,Yinchuan 750021,China
  • Online:2020-08-25 Published:2020-08-25

Abstract: The pentatricopeptide repeats(PPR)proteins involve in post-transcriptional modifications and editing of specific single-stranded RNAs in the nucleus and organelles via their localization in various organelles,and they play important roles in series events of plant growth and development. In this study,Lb_PPR1 gene was cloned from the fertile line‘Ningqi 1’and the male-sterile line‘Ningqi 5’. Bioinformatics analysis was used to predict the physico-chemical properties,subcellular localization,conserved domain,protein structure and phylogenetic evolution of Lb_PPR1 encoded protein. The results indicated that Lb_PPR1 gene contained a 1 977 bp open reading frame encoding 658 amino acids in both ‘Ningqi 1’and ‘Ningqi 5’,but there were 20 bp and 14 aa differences between the two cultivars. Lb_PPR1 protein was predicted to have 14 tandem repeats,belonged to PLS family,and its subcellular localization was in cell membrane and cytoplasm. Secondary and tertiary structure predictions showed that Lb_PPR1 protein was dominated by α-helix. Phylogenetic analysis showed that Lb_PPR1 had the closest relationship with PPR in Capsicum annuum and Nicotiana attenuate. Quantitative real-time PCR(qRT-PCR)was performed to determine the expression pattern of Lb_PPR1 gene in different organs and different developmental stages of anther. The results showed that Lb_PPR1 was expressed in different tissues of Lycium barbarum,but highest in the fertile line’s flower buds,its expression in the male-sterile line‘Ningqi 5’were all significantly lower than the fertile line‘Ningqi 1’. The above results indicate that Lb_PPR1 gene maybe related to the development of flower organs in Lycium barbarum.

Key words: Lycium barbarum, Lb_PPR1, bioinformatics analysis, gene expression pattern

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