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园艺学报 ›› 2019, Vol. 46 ›› Issue (10): 1960-1972.doi: 10.16420/j.issn.0513-353x.2019-0036

• 研究论文 • 上一篇    下一篇

菜薹品质性状与SSR标记的关联分析

夏岩石1,张润霖1,卢宇鹏1,李荣华1,李光光2,张 华2,郭培国1,*   

  1. 1广州大学生命科学学院,作物抗逆国际合作研究中心,广州 510006;2广州市农业科学研究院,广州 510308
  • 出版日期:2019-10-25 发布日期:2019-10-25
  • 基金资助:
    广东高校省级重点平台和重大科研项目(2015KGJHZ015);广东省科技计划项目(2016B020201001);广东省自然科学基金项目(2015A030310136,2015A030313500,2019A1515011587)

Association Analysis of Quality Traits with SSR Markers in Flowering Chinese Cabbage

XIA Yanshi1,ZHANG Runlin1,LU Yupeng1,LI Ronghua1,LI Guangguang2,ZHANG Hua2,and GUO Peiguo1,*   

  1. 1International Crop Research Center for Stress Resistance,College of Life Sciences,Guangzhou University,Guangzhou 510006,China;2Guangzhou Academy of Agricultural Sciences,Guangzhou 510308,China
  • Online:2019-10-25 Published:2019-10-25

摘要: 为挖掘与菜薹品质性状相关的分子标记,选用具有多态性的84个SSR标记分析了81份菜薹种质材料的遗传多样性,并采用TASSEL3.0软件中混合线性模型(MLM)对菜薹群体材料的单株质量、叶绿素含量、维生素C含量、可溶性总糖含量、可溶性蛋白含量和硝酸盐含量等6个品质性状进行关联分析。结果显示,84个SSR标记在81份菜薹种质材料中共检测出310个等位位点,引物多态信息量(PIC)在0.1878 ~ 0.9902,平均值为0.6119;81份菜薹种质材料的遗传相似系数(GS)在0.4742 ~ 0.8958,平均值为0.6294;在GS值为0.596水平上,81份菜薹种质可聚为4个类群。关联分析显示,10个等位位点与菜薹4个品质性状显著相关(P < 0.01),对表型变异的贡献率为8.59% ~ 11.50%,其中5个等位位点表现为增效表型效应,其余5个等位位点为减效表型效应。基于等位位点的分析结果,鉴定出典型的载体材料11份,分别携带有4 ~ 8个优异等位位点。本研究发掘的与菜薹品质性状相关的优异等位位点及载体材料,可为高品质菜薹的分子标记辅助育种提供参考。

关键词: 菜薹, 品质性状, SSR标记, 关联分析

Abstract: In order to identify molecular markers loci related to quality traits of flowering Chinese cabbage and promote marker-assisted breeding in flowering Chinese cabbage,84 polymorphic SSRs were used to genotype and analyze genetic diversity of 81 flowering Chinese cabbage accessions. Association analysis between genotypic data and six quality traits was performed by the method of MLM(mixed linear model)in Tassel 3.0. The results showed that 310 alleles were detected in 81 accessions,and the polymorphic information content(PIC)ranged from 0.1878 to 0.9902,with an average values of 0.6119. The genetic similarity coefficient(GS)of 81 flowering Chinese cabbage accessions was between 0.4742 to 0.8958,with an average of 0.6294. These accessions could be clustered into four groups at the GS value of 0.596. A total of 10 loci associated with quality traits were detected at P < 0.01 level by association analysis, with explained variance ranging from 8.59% to 11.50%,of which 5 loci had positive effect on phenotype traits,and the rest were negative effect. Based on the results of elite alleles analysis,11 typical materials with 4–8 elite alleles were identified. These elite alleles could be useful for marker-assisted selection breeding in flowering Chinese cabbage.

Key words: flowering Chinese cabbage, quality traits, SSR marker, association analysis

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