园艺学报 ›› 2019, Vol. 46 ›› Issue (3): 549-558.doi: 10.16420/j.issn.0513-353x.2018-0331

• 研究论文 • 上一篇    下一篇



  1. (1连云港市农业科学院花卉研究中心,江苏连云港 222000;2中国林业科学研究院亚热带林业研究所,浙江省林木育种技术研究重点实验室,杭州 311400;3江苏百草园园艺工程有限公司,江苏连云港 222000)
  • 出版日期:2019-03-25 发布日期:2019-03-25

Analysis of Potential Double Resistances for 49 Camellia Cultivars Under Low Temperature and Salt Stress

WANG Jiangying1,2,ZHAO Tongli1,ZHU Pengbo1,LI Jiyuan2,TANG Xueyan1,SUN Mingwei1,HUI Linchong1,and SHAO Xiaobin1,3,*   

  1. (1Lianyungang Academy of Agricultural Sciences,Flower Research Center,Lianyungang,Jiangsu 222000,China;2Research Institute of Subtropical Forestry of Chinese Academy of Forestry,Zhejiang Provincial Key Laboratory of Tree Breeding,Hangzhou 311400,China;3Jiangsu Baicaoyuan Horticultural Engineering Co.,LTD,Lianyungang,Jiangsu 222000,China)
  • Online:2019-03-25 Published:2019-03-25

摘要: 根据杜鹃红山茶(Camellia azalea)抗坏血酸过氧化物酶(ascorbate peroxidase,APX)基因(CaAPX1,GenBank登录号:KP635267)序列,设计实时荧光定量PCR引物,并对4 ℃低温和200 mg ? L-1 NaCl胁迫下杜鹃红山茶CaAPX1表达量进行分析,发现该基因能够在低温和NaCl胁迫下应激上调表达。实时荧光定量PCR分析发现,CaAPX1在49个山茶品种中均得到表达,但表达量有差异。通过田间抗寒耐盐指数和APX1表达量隶属函数值对49个山茶品种抗寒和耐盐双抗能力进行分析,其中Ⅰ类抗寒和耐盐能力强的有4个品种,Ⅱ类抗寒和耐盐能力中等的有11个品种,Ⅲ类抗寒和耐盐能力弱的有34个品种。

关键词: 山茶, 抗寒, 耐盐, 双抗, 表达分析, 综合评价

Abstract: On the basic of CaAPX1(GenBank accession ID:KP635267) sequence of Camellia azalea,a pair of qPCR primer was designed and synthesized. Fluorescent quantitative Real-time PCR analysis indicated that the expression levels of CaAPX1 in leaves of Camellia azalea were both upregulated under 4 ℃ and 200 mg ? L-1 NaCl treatment,reaching peaks at 8 h and 12 h treatment respectively,which indicated that CaAPX1 could be expressed in camellia plants after separately exposed to cold and salt stresses. It was also found that CaAPX1 gene was expressed in the 49 camellia cultivars,but the expression levels were different. Furthermore,on the basis of comprehensive evaluation,49 camellia cultivars were evaluated for their potential of double resistance through the field investigation and CaAPX1 relative expression,the first category(typeⅠ)with strong cold-salt resistance including four camellias;the second category(typeⅡ)to moderate cold-salt resistance including eleven camellias;while the third category (type Ⅲ)with weaker cold-salt resistance including thirty four camellias.

Key words: Camellia japonica, cold resistance, salt tolerance, double resistances, expression analysis, comprehensive evaluation