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园艺学报 ›› 2015, Vol. 42 ›› Issue (6): 1112-1120.doi: 10.16420/j.issn.0513-353x.2015-0156

• 蔬菜 • 上一篇    下一篇

四倍体西瓜抗枯萎病生理小种1分子标记辅助选择技术研究

焦荻,任毅,宫国义,张海英,郭绍贵,张洁,许勇   

  1. 1北京农学院植物科学技术学院,北京 102206;2北京市农林科学院蔬菜研究中心,农业部华北地区园艺作物生物学与种质创制重点实验室,北京蔬菜种质改良实验室,北京 100097
  • 出版日期:2015-06-25 发布日期:2015-06-25
  • 基金资助:
    国家现代农业产业技术体系建设专项资金项目(CARS-26);国家科技支撑计划项目(2013BAD01B04,2012BAD02B03);北京市科委项目(Z131100003113012,KJCX20140111)

Mocecular Marker-assisted Selection for Resistance to Fusarium oxysporum f. sp. niveum Race 1 in Tetraploid Watermelon

JIAO Di, REN Yi, GONG Guo-Yi, ZHANG Hai-Ying, GUO Shao-Gui, ZHANG Jie, XU Yong   

  1. 1Plant Science and Technology College,Beijing University of Agriculture,Beijing 102206,China;2National Engineering Research Center for Vegetables,Beijing Academy of Agriculture and Forestry Sciences,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China),Ministry of Agriculture,Beijing Vegetable Germplasm Improvement Lab,Beijing 100097,China
  • Online:2015-06-25 Published:2015-06-25

摘要: 基于已获得的二倍体西瓜与抗枯萎病基因Fon-1紧密连锁的分子标记,进一步完成了Fon-1基因的精细定位。利用两个四倍体西瓜材料(易感病的NF3为受体,抗病的JH为供体),构建以NF3为轮回亲本的回交群体,使用新开发出的SNP标记对各世代群体进行分子标记辅助选择,及苗期抗病性接种鉴定。结果发现,在分离世代群体中通过接种鉴定获得抗病株比例较分子标记检测值低12.64% ~ 15.34%,这可能是由于基因剂量效应造成的。抗病基因杂合位点对枯萎病抗性依次为:三显体(AAAa)> 二显体(AAaa)> 单显体(Aaaa)。目前分子标记尚无法检测杂合基因型的剂量效应,容易将感病的单显体(Aaaa)误判为抗病株。在构建的673株BC1F2代自交群体中检测到29株纯合基因型(AAAA)抗病单株,占总检测株数的4.31%,与苗期抗病性接种鉴定结果符合度达到100%。

关键词: 西瓜, 四倍体, 枯萎病生理小种1, 抗性, 分子标记辅助选择

Abstract: Based on the molecular markers tightly linked to Fon-1 for Fusarium oxysporum f. sp. niveum race 1 resistance in the diploid watermelon developed in our laboratory,the fine mapping of Fon-1 was finished in this study. A backcross population of susceptible recurrent parent NF3 and resistant donor parent JH was constructed. The newly developed SNP marker was used for Fon-1 resistance marker- assisted selection and seedling inoculation identification in different generations. The results showed that the ratio of resistant plants post-inoculated was 12.64%–15.34% lower than the ratio of resistantgenotype detected by molecular marker. The reason is that because of gene dosage effect,different heterozygosity genotype have different resistance in tetraploid watermelon as follow:AAAa > AAaa > Aaaa. At present the molecular marker could not detect the gene dosage effect or identify the susceptible genotype Aaaa. In the 673 plants BC1F2 population,we detected 29 homozygous resistant genotype(AAAA),accounting for 4.31% of the total number of plants. All of the 29 homozygous plants showed Fon-1 resistance by screening with inoculation of Fon race 1.

Key words: watermelon, tetraploid, Fusarium oxysporum f. sp. niveurn race 1, resistance, molecular marker-assisted selection

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