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园艺学报 ›› 2014, Vol. 41 ›› Issue (8): 1642-1650.

• 观赏植物 • 上一篇    下一篇

牡丹PobZIP1在种子发育中的表达及其与ABA含量的关系

张 萍,王顺利,朱富勇,薛璟祺,刘传娇,任秀霞,张秀新*   

  1. (中国农业科学院蔬菜花卉研究所,北京 100081)
  • 收稿日期:2014-03-03 出版日期:2014-08-25 发布日期:2014-08-25
  • 基金资助:
    国家公益性行业(农业)科研专项(201203071);中国农业科学院科技创新工程项目(2013-2014);中国农业科学院院所基金项目(2060302-2-12);国家‘863’计划项目(2011AA10020703);农业部园艺作物生物学与种质创制重点实验室项目

Expression Analysis of the PobZIP1 Transcription Factor Gene in Developmental Seeds from Tree Peony and Its Relationship with ABA Content

ZHANG Ping,WANG Shun-li,ZHU Fu-yong,XUE Jing-qi,LIU Chuan-jiao,REN Xiu-xia,and ZHANG Xiu-xin*   

  1. (Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
  • Received:2014-03-03 Online:2014-08-25 Published:2014-08-25

摘要: 采用RT-PCR方法从‘凤丹’牡丹(Paeonia ostii‘Fengdan’)种子中克隆得到1个与ABA信号途径相关的转录因子基因PobZIP1,并利用超高效液相色谱—串联质谱(UPLC–MS/MS)的方法测定了不同发育时期种子的ABA含量。PobZIP1 cDNA全长957 bp,编码318个氨基酸,其编码的蛋白C末端含有bZIP转录因子的典型结构域,GenBank登录号为KJ009392。PobZIP1蛋白与拟南芥bZIP转录因子聚类分析显示,PobZIP1转录因子属于拟南芥bZIP转录因子的A亚族,与AtbZIP39/ABI5聚在一起。与其他物种bZIP蛋白的系统进化分析表明,牡丹PobZIP1与蔷薇科的苹果bZIP亲缘关系最近。SqRT-PCR结果表明:PobZIP1在‘凤丹’牡丹根、茎、叶和花芽中均有表达,其中花芽中的表达量最低,根、茎、叶中的表达量相近。在种子发育过程中,PobZIP1的表达呈现出先增加后降低的趋势。ABA含量测定结果显示,随着种子的发育ABA含量迅速上升,达到最大值后缓慢下降并保持在较高水平。推测种子发育后期,较高含量的ABA和表达相对较高的PobZIP1可能是诱导种子休眠形成的原因。

关键词: 牡丹, 种子, ABA含量, PobZIP1, 半定量RT-PCR

Abstract: One important transcription factor gene PobZIP1 involved in ABA signaling was obtained by RT-PCR,first time in Paeonia ostii‘Fengdan’. And the abscisic acid(ABA)content in seeds of ‘Fengdan’from different developmental stages was analyzed by ultra-performance liquid chromatography/ tandem mass spectrometric(UPLC-MS/MS)method. The full length of the coding sequence of PobZIP1 was 957 bp,encoding a protein of 318 amino acid residues with a characteristic bZIP domain in the C-terminal domain. The GenBank accession number is KJ009392. A phylogenetic analysis with otherbZIP proteins from Arabidopsis thaliana revealed that PobZIP1 belongs to the group A of bZIP transcription factors,and it was closely related to AtbZIP39/ABI5. Then another phylogenetic tree was constructed with other bZIPs from different species,the results suggested that PobZIP1 be closely related to bZIP protein in Malus × domestica. Specific tissue and organ expression analysis showed that PobZIP1 could express in leaves,stems,roots and buds,while low expression in buds. The PobZIP1 expression pattern of different developmental stages of seeds was up-down trend. The results suggested that ABA content increased rapidly in early developmental stages of seeds,then decreased and kept relatively high level. It has been hypothesized that the high level of ABA and PobZIP1 in late developmental stages could induce seeds to dormancy.

Key words: tree peony, seed, ABA content, PobZIP1, semi-quantitative RT-PCR

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