黄瓜及其近缘种对南方根结线虫的抗性及酶响应变化的研究

园艺学报 ›› 2009, Vol. 36 ›› Issue (12): 1755-1760.

黄瓜及其近缘种对南方根结线虫的抗性及酶响应变化的研究

叶德友^{1, 2}; 钱春桃¹ ; 贾媛媛¹ ; 张燕霞¹; 陈劲枫^1*

(¹ 作物遗传与种质创新国家重点实验室,南京大学园艺学院, 南京 210095; ² 甘肃省农业科学院蔬菜研究所, 兰州730070)

收稿日期:2009-06-12 修回日期:2009-12-07 出版日期:2009-12-25 发布日期:2009-12-25
通讯作者: 陈劲枫

Cucumber and Its Related Species for Resistance to the Sourthern Root-knotNematode Meloidogyne incognita and Respond to Changes of Enzyme

YE De-you^{1, 2} ; QIAN Chun-tao¹ , J IA Yuan-yuan¹ , ZHANG Yan-xia¹ , and CHEN Jin-feng^1*

(¹ State Key Laboratory of Crop Genetics and Germ plasm Enhancement, College of Horticulture, Nanjing Agricultural University,Nanjing 210095, China; ² Institute of Vegetables, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China)

Received:2009-06-12 Revised:2009-12-07 Online:2009-12-25 Published:2009-12-25
Contact: CHEN Jin-feng

摘要/Abstract

摘要： 选取6份黄瓜品种和2份近缘种(非洲角和酸黄瓜) 进行了南方根结线虫病抗性鉴定, 2份
近缘野生种高抗南方根结线虫病, 6份黄瓜品种为感病类型。接种前后根系酶活性与病情指数相关性分析表明, 超氧化物歧化酶( SOD) 、过氧化物酶( POD) 、苯丙氨酸解氨酶( PAL) 活性变化与其病情指数分别呈显著正相关( r = 0.8278*) 、极显著负相关( r = - 0.9679**
) 和显著负相关( r = - 0.8499*) 。POD同工酶电泳分析表明, 抗病材料酸黄瓜较未接种对照增加了3 条酶带(相对迁移率Rf = 0.126, 0.188,0.944) , 而感病材料北京截头只增加了1条弱带(Rf = 0.396) 。SOD、POD及PAL活性水平可作为甜瓜属作物抗根结线虫病鉴定的生化指标及常规抗性鉴定方法的补充。

关键词: 黄瓜, 甜瓜属, 近缘种, 南方根结线虫, 抗性, 酶

Abstract: The eight Cucum is germplasms were selected for evaluation of their resistance to the root-knot nematodes Meloidogyne incognita. The results showed that the two Cucumis wild relatives were highly resistant to Meloidogyne incognita, and the six varieties of cucumber were all susceptible. The correlation analysis between the rates of changes of superoxide dismutase ( SOD) , peroxidase ( POD) , phenylalanine ammonialyase ( PAL) in roots before and after inoculation and disease index for Cucumis species tested was performed.The results showed that there was a significant positive correlation of SOD ( r = 0.8278*),highly significant negative correlation of POD ( r = - 0.9679** )
and significant negative correlation of PAL(R=-0.8499*) associated with the disease index, respectively. POD isoenzyme electrophoresis analysis showed that there were three more enzyme bands (Rf = 0.126, 0.188, 0.944) in the resistant genotype ‘C. hystrix’, but only one weak band (Rf = 0.396) in the suscep \tible genotype‘Beijing Jietou’when compared to noninoculated control. The level of SOD, POD and PAL activities can be used as the biochemical markers and the supplement for the conventional method of evaluation for resistance to the root-knot nematodes in Cucumis species.

Key words: cucumber, Cucumis, related species, Meloidogyne incognita, resistance, enzyme

中图分类号:

叶德友;;钱春桃;贾媛媛;张燕霞;陈劲枫. 黄瓜及其近缘种对南方根结线虫的抗性及酶响应变化的研究[J]. 园艺学报, 2009, 36(12): 1755-1760.

YE De-you;;QIAN Chun-tao;J IA Yuan-yuan;ZHANG Yan-xia;and CHEN Jin-feng. Cucumber and Its Related Species for Resistance to the Sourthern Root-knotNematode Meloidogyne incognita and Respond to Changes of Enzyme[J]. ACTA HORTICULTURAE SINICA, 2009, 36(12): 1755-1760.

[1]	李瑞雅, 宋程威, 牛童非, 魏祯祯, 郭丽丽, 侯小改. ‘海黄’牡丹花挥发性物质释放规律及PsGDS的克隆与表达分析[J]. 园艺学报, 2023, 50(2): 331-344.
[2]	余阳俊, 汪维红, 苏同兵, 张凤兰, 张德双, 赵岫云, 于拴仓, 李佩荣, 辛晓云, 王姣. 抗根肿病耐抽薹大白菜新品种‘京春CR3’[J]. 园艺学报, 2022, 49(S2): 87-88.
[3]	王丽丽, 王鑫, 吴海东, 温蔷, 杨晓飞. 抗根肿病大白菜新品种‘辽白28’[J]. 园艺学报, 2022, 49(S2): 89-90.
[4]	罗天宽, 吴海涛, 张圣美, 黄宗安, 孙继, 水德聚, 陈先知. 黄瓜新品种‘瓯翠1号’[J]. 园艺学报, 2022, 49(S2): 125-126.
[5]	王鹤冰, 向华丰, 陈新中, 张生, 张洪成. 华南型黄瓜新品种‘新燕095’[J]. 园艺学报, 2022, 49(S1): 79-80.
[6]	许春梅, 张作标, 柳景兰, 王昕, 杨龙, 赵丹, 刘思宇, 贾云鹤, 孟雪娇, 崔嵩岑. 黄瓜新品种‘绿春2号’[J]. 园艺学报, 2022, 49(S1): 81-82.
[7]	张利东, 黄洪宇, 孔维良, 李加旺, 李愚鹤, . 华北型黄瓜新品种‘津优355’[J]. 园艺学报, 2022, 49(S1): 83-84.
[8]	王惠哲, 杨瑞环, 邓强, 曹明明, 李淑菊, . 抗黑星病黄瓜新品种‘津冬369’[J]. 园艺学报, 2022, 49(S1): 85-86.
[9]	侯潞丹, 赵梦然, 黄晨阳, 张金霞. 交替氧化酶增强糙皮侧耳高温耐受性的分子机制[J]. 园艺学报, 2022, 49(9): 1922-1934.
[10]	黄玲, 胡先梅, 梁泽慧, 王艳平, 产祝龙, 向林. 郁金香花青素合成酶基因TgANS的克隆与功能鉴定[J]. 园艺学报, 2022, 49(9): 1935-1944.
[11]	刘朝阳, 廖志婵, 路鑫鑫, 何业华. 菠萝类纤维素合成酶CslD家族基因鉴定及AcoCslD2a功能分析[J]. 园艺学报, 2022, 49(8): 1650-1662.
[12]	杨禹妍, 段新圆, 何治霖, 邴起浩, 陈锁英, 刘晓曼, 曾明, 刘小刚. 金柑UDP-鼠李糖合成酶基因的克隆与功能解析[J]. 园艺学报, 2022, 49(8): 1663-1672.
[13]	邱子文, 刘林敏, 林永盛, 林晓洁, 李永裕, 吴少华, 杨超. 千层金MbEGS基因的克隆与功能分析[J]. 园艺学报, 2022, 49(8): 1747-1760.
[14]	聂鑫淼, 栾恒, 冯改利, 王超, 李岩, 魏珉. 硅营养和嫁接砧木对黄瓜幼苗耐冷性的影响[J]. 园艺学报, 2022, 49(8): 1795-1804.
[15]	陶鑫, 朱荣香, 贡鑫, 吴磊, 张绍铃, 赵建荣, 张虎平. 梨果糖激酶基因PpyFRK5在果实蔗糖积累中的作用[J]. 园艺学报, 2022, 49(7): 1429-1440.