红掌愈伤组织诱导和芽的分化

园艺学报 ›› 2003, Vol. 30 ›› Issue (1): 107-109.

红掌愈伤组织诱导和芽的分化

兰芹英¹;李启任²;何惠英¹;张艳军¹;解星云³

(¹中国科学院西双版纳热带植物园, 勐腊666303 ;² 云南大学生物技术系, 昆明650091 ;³云南省茶叶科学研究所,勐海666201)

收稿日期:2001-12-10 修回日期:2002-06-21 出版日期:2003-02-25 发布日期:2003-02-25

The Callus Induction of Anthurium andraeanum Linden and Bud Differentiation

Lan Qinying¹;Li Qiren²;He Huiying¹;Zhang Yanjun¹;Xie Xingyun³

(¹Xishuangbanna Tropic Botanical Garden , The Academy of Sciences , Mengla 666303 , China ;² Department of Biology Technology,Kunming 650091 , China ;　³Tea Reserch Institute of Yunnan Province , Menghai 666201 , China)

Received:2001-12-10 Revised:2002-06-21 Online:2003-02-25 Published:2003-02-25

摘要/Abstract

摘要： 对影响红掌愈伤组织诱导及芽分化的几个因素进行了研究。同一成熟度的外植体, 其叶柄愈
伤组织诱导率、芽分化率、分化时间均明显优于叶片。不同放置方式和光照时间对叶片愈伤组织的诱导亦有影响, 叶背向下放置, 光照时间24 h/ d 和10 h/ d 愈伤组织诱导率较高, 分别为100 %、97 %。光照时间对叶柄愈伤组织诱导无显著影响, 但光照24 h/ d 和10 h/ d 较无光照处理的明显促进芽的分化。叶柄培养以N6 , KC 和1/ 2 MS 培养基为佳。叶片培养则以P , N6 和1/ 2 MS 为好。以未展叶叶柄为外植体, 从接种到丛芽分化共49 d , 较已有报道提前11～31 d。

关键词: 红掌, 愈伤组织, 诱导, 芽分化

Abstract: The factors that influence the callus induction and bud differentiation of Anthurium andraeanum Linden were studied. Petiole showed significantly better results than blade in callus induction , bud differentiation and duration for bud formation. The callus induction on blade was influenced by the way of placement on medium and period for lighting , and the highest percentage (100 % and 97 %) for callus induction were recorded under the condition that the downside of blade were against the medium and with 24 h/ d and 10 h/ d light . Differnet light treatments did not affect the callus induction of petiole significantly. However , the 24 h/ d and 10 h/ d light treatment obviously improved the bud differentiation compared to the treatment with no light . Promising results among several mediums tested were recorded : N6 , KC and 1/ 2 MS for petiole and P , N6 and 1/ 2 MS for blade , respectively. The time from explant to bud differentiation were 49 d , which was 11 - 31 d earlier than time previously reported.

Key words: Anthurium andraeanum Linden, Inducing of callus, Differentiating bud

中图分类号:

S 68

兰芹英;李启任;何惠英;张艳军;解星云. 红掌愈伤组织诱导和芽的分化[J]. 园艺学报, 2003, 30(1): 107-109.

Lan Qinying;Li Qiren;He Huiying;Zhang Yanjun;Xie Xingyun. The Callus Induction of Anthurium andraeanum Linden and Bud Differentiation[J]. ACTA HORTICULTURAE SINICA, 2003, 30(1): 107-109.

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