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园艺学报 ›› 2020, Vol. 47 ›› Issue (10): 1999-2008.doi: 10.16420/j.issn.0513-353x.2019-0996

• 研究报告 • 上一篇    下一篇

‘越心’草莓组培突变体着色差异的分子机理初探

杨肖芳1,张祖瑛2,苗立祥1,张豫超1,沈 岚3,秦巧平4,*,蒋桂华1,*   

  1. 1浙江省农业科学院园艺研究所,杭州 310021;2浙江大学园艺系,杭州 310058;3宁波市农业科学研究院,浙江宁波 315040;4上海应用技术大学生态技术与工程学院,上海 201418
  • 出版日期:2020-10-25 发布日期:2020-11-10
  • 基金资助:
    浙江省科技厅重点研发计划项目(2016C02052-8);浙江省团队特派员项目;浙江省公益技术研究计划项目(LGN20C150008);宁波市“科技创新2025”重大专项(2019B10021)

Preliminary Analysis on the Molecular Differences of Fruit Colouring Between Yuexin Strawberry and Its Somaclonal Mutation

YANG Xiaofang1,ZHANG Zuying2,MIAO Lixiang1,ZHANG Yuchao1,SHEN Lan3,QIN Qiaoping4,*,and JIANG Guihua1,*   

  1. 1Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;2Department of Horticulture,Zhejiang University,Hangzhou 310058,China;3Ningbo Academy of Agricultural Sciences,Ningbo,Zhejiang 315040,China;4School of Ecological Technology and Engineering,Shanghai Institute of Technology,Shanghai 201418,China
  • Online:2020-10-25 Published:2020-11-10

摘要: 初步探讨了在‘越心’草莓(Fragaria × ananassa‘Yuexin’)茎尖离体培养再生植株中发现的稳定变异突变体着色差异的分子机理。分析结果显示,突变体与‘越心’在植株形态、始花期、始果期、始熟期、单果质量、平均株产、果形、风味等方面均无明显差异;而突变体外果皮色泽较淡,L*值较高,a*值较低,H色度角值较大,颜色指数CIRG显著小,且果肉不着色(‘越心’果肉红色)。突变体果实总花青苷含量为‘越心’的13.2%,其中以天竺葵素–3–O–葡萄糖苷(Pg3G)含量差异最大,突变体Pg3G含量仅为‘越心’的12.9%。花青苷合成途径结构基因FaF3H、FaCHS、FaDFR、FaANS、FaCHI、Fa3GT的表达水平在突变体中显著降低,这可能是突变体成熟果实花青苷水平显著低于‘越心’的直接原因。FaMYB10和FaMYB1在突变体中的表达水平显著下降,且FaMYB10在几个主成分中的特征向量绝对值均显著较高,进一步证实了MYB10是草莓成熟过程中花青苷合成的类黄酮或苯丙烷类化合物途径主要调节因子之一。提取叶片基因组DNA进行基于KASP标记的基因分型结果显示,74对KASP引物在‘越心’草莓及其突变体中的基因分型不存在差异。本结果初步证实突变导致参与调控花青苷合成途径的关键基因表达发生显著下调从而使花青苷积累显著减少,果实着色变淡。

关键词: 草莓, 组培, 突变体, 花青苷, 果实品质

Abstract: Fragaria × ananassa‘Yuexin’is a new strawberry cultivar selected by our group. The Yuexin mutant is a stable somaclonal variation from Yuexin found in the regeneration plants differentiated stem apex. The results showed that there were no significant differences between Yuexin and its mutant in plant morphology,onset of blooming and fruit,onset of fruit ripening,average fruit weight and plant yield,fruit shape and fruit flavor,etc. However,compared with Yuexin,the pricarp of its mutant was brighter with higher L* and lower a*,greater H and less CIRG index,and the mutant had colorless pulp. The total content of anthocyanin in the mutant was only 13.2% of Yuexin,of which the largest difference was the content of geranium-3-O-glucoside(Pg3G),the content of Pg3G in the mutant was only 12.9% of Yuexin. The expression levels of the structural genes FaF3H,FaCHS,FaDFR,FaANS,FaCHI and Fa3GT involved in the anthocyanin synthesis pathway were significantly reduced in the mutant fruit,indicating the possible direct factors that resulting in the lower levels of anthocyanin in the mutant. The expression levels of the transcription factors FaMYB10 and FaMYB1 in Yuexin mutant were decreased significantly,and the eigenvector absolute values of FaMYB10 in several principal components were significantly higher than other factors,which further proved that MYB10 was one of the main regulating factors involved in flavonoids/phenylpropanoids of anthocyanin pathway during strawberry maturation. KASP analysis using leaf genomic DNA showed that there was no genotype differentiation between Yuexin and its mutant based on 74 primer pairs amplification. The results in this study confirmed that the Yuexin mutant was genetically stable,and the mutation caused the significant decrease in the expression of key genes involved in the regulation of anthocyanin synthesis,resulting in decreasing of anthocyanin accumulation and reducing fruit coloration.

Key words: strawberry, tissue culture, somaclonal mutation, anthocyanin, fruit quality

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