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http://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2020, Vol. 47 ›› Issue (6): 1165-1171.doi: 10.16420/j.issn.0513-353x.2019-0872

• 研究报告 • 上一篇    下一篇

桃乙烯应答因子PpERF1a的克隆与功能分析

刘 勇1,*,王泽琼1,2,*,龚林忠1,**,王富荣1,王会良1,艾小艳1,何华平1,**   

  1. 1湖北省农业科学院果树茶叶研究所,武汉 430064;2武汉生物工程学院,武汉 430415
  • 出版日期:2020-06-25 发布日期:2020-06-25
  • 基金资助:
    国家重点研发计划项目(2018YFD0201400);国家现代农业产业技术体系建设专项资金项目(CARS-31-02A);农业部华中作物有害生物综合治理重点实验室开放基金项目(2018ZTSJJ6);农业部农业微生物资源利用华中区域科学观测试验站联合开放基金项目(JF-NBCOES-1804)

Cloning and Functional Analysis of ERF Transcription Factor Gene PpERF1a in Peach

LIU Yong1,*,WANG Zeqiong1,2,*,GONG Linzhong1,**,WANG Furong1,WANG Huiliang1,AI Xiaoyan1,and HE Huaping1,**   

  1. 1Institute of Fruit and Tea,Hubei Academy of Agricultural Sciences,Wuhan 430064,China;2Wuhan Institute of Bioengineering,Wuhan 430415,China
  • Online:2020-06-25 Published:2020-06-25

摘要: 以‘丰光’油桃嫩梢组织为试材,克隆了1个ERF家族基因并命名为PpERF1a(ppa018178m)。该基因全长为600 bp,编码199个氨基酸,含有1 个典型的AP2 结构域。亚细胞定位结果显示PpERF1a定位于细胞膜和细胞核。在拟南芥中超量表达PpERF1a,共获得15个阳性转基因株系。与对照相比,转基因植株出现发育不良的表型,其中6株持续长出莲座叶,但不抽薹;6株能够抽薹开花,但不结实且生长势明显弱于对照;3株能够开花结实,但种子产量极低。这些结果表明PpERF1a在转基因拟南芥中具有调控生长发育的功能,为后期在桃中开展PpERF1a的功能研究提供了有益的启示。

关键词: 桃, 乙烯应答因子, 亚细胞定位, 功能分析

Abstract: In this study,an ethylene response factor was cloned from‘Fengguang’nectarine and named as PpERF1a(ppa018178m). The full length of PpERF1a was 600 bp,which encoded 199 amino acids. PpERF1a contained a single AP2 domain, which was consistent with the ERF families’ features. Subcellular localization assays showed that PpERF1a was targeted to nucleus and cell membrane. Fifteen transgenic Arabidopsis plants overexpressing PpERF1a were generated. Comparing with the wild type Arabidopsis plants,transgenic plants showed obvious stunted phenotype. Six transgenic plants kept on producing rosette leaves without bolting or producing seeds. Six transgenic plants bolted and flowered,but no seed. Three transgenic plants could flower and produce seed,but the seed yield of the transgenic plants was obviously lower than that of the wild type. These results showed that PpERF1a gene regulated the growth and development of transgenic Arabidopsis plants. This study provides a technical basis for further analysis the functions of PpERF1a gene in peach growth and development.

Key words: peach, ethylene response factor, subcellular localization, function analysis

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