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园艺学报 ›› 2020, Vol. 47 ›› Issue (5): 875-892.doi: 10.16420/j.issn.0513-353x.2019-0735

• 研究论文 • 上一篇    下一篇

辣椒MYB基因家族的鉴定及与辣味关系分析

居利香,雷 欣,赵成志,舒黄英,汪志伟,成善汉*   

  1. 海南大学园艺学院,海口 570228
  • 出版日期:2020-05-25 发布日期:2020-05-25
  • 基金资助:
    国家自然科学基金项目(31760578)

Identification of MYB Family Genes and Its Relationship with Pungency of Pepper

JU Lixiang,LEI Xin,ZHAO Chengzhi,SHU Huangying,WANG Zhiwei,and CHENG Shanhan*   

  1. Collgege of Horticulture,Hainan University,Haikou 570228,China
  • Online:2020-05-25 Published:2020-05-25

摘要: 利用生物信息学方法从辣椒全基因组数据库中筛选出172个MYB转录因子,并对其序列特征、染色体定位、进化关系、蛋白质保守基序和基因结构等进行综合分析,同时通过辣椒果实不同发育阶段胎座组织转录组测序筛选与辣味可能相关的MYB基因。结果表明,辣椒MYB有1R、2R(R2R3)、3R、6R等类型,其中R2R3型居多,结构域分析表明其具有典型的W型R2R3保守基序;获取20个motif元件并进行位置分析,发现同一支MYB蛋白具有相似的结构特征;进一步与拟南芥聚类得到37个类群,推测具有多种生物学功能。转录组测序发现,CaMYB98、CaMYB168等12个MYB表达量符合‘黄魔王’辣椒果实胎座组织在不同发育期的辣椒素含量变化规律,推测其可能通过特异位点的结合来上调或下调相关蛋白的表达,从而达到对辣椒素代谢路径的调控,可作为辣味调控的重要候选基因进一步研究。

关键词: 辣椒, MYB转录因子, 转录组测序, 辣椒素含量

Abstract: One hundred and seventy-two MYB transcription factors were extracted from the pepper genome database by bioinformatics methods,and there was a comprehensive analysis of the sequence characteristics,the chromosomal location,evolutionary relationships,protein conservative motif and genetic structure at the same time. What’s more,the technology of RNA-seq was used to screen MYB genes that might be related to the expression of spiciness genes. The results showed that there were 1R,2R(R2R3),3R,6R and other types of pepper MYB,among which R2R3 was the majority. Furthermore structural domain analysis showed that they have typical W type R2R3 conservative motifs. Subsequently,about twenty motif elements were obtained,and position analysis showed that MYB proteins from the same branch seem to have similar structural characteristics. A total of 37 groups were obtained through clustering with Arabidopsis thaliana,suggesting that the pepper MYBs may have various biological functions. The RNA-seq results indicated that the expression of twelve MYB,such as CaMYB98 and CaMYB168,were consistent with the accumulation of capsaicin content in fruit placenta tissues of‘Huangmowang’at different development stages. Thus it was predicted that they can regulate the metabolic pathwayof capsaicin by up- or down-regulating the expression of related proteins by binding to specific sites,which can be further studied as important candidate genes for the regulation of pungency.

Key words: Capsicum, MYB transcription factors, transcriptome sequencing, capsaicin content

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