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园艺学报 ›› 2020, Vol. 47 ›› Issue (4): 643-652.doi: 10.16420/j.issn.0513-353x.2019-0599

• 研究论文 • 上一篇    下一篇

茄子花药开裂相关基因SmDAD1 启动子的克隆及功能分析

张少伟1,*,袁 超1,*,牛 义1,*,汤青林1,魏大勇1,王永清2,田时炳2,杨 洋2,**,王志敏1,**   

  1. 1西南大学园艺园林学院,南方山地园艺学教育部重点实验室,重庆市蔬菜学重点实验室,重庆 400715;2重庆市农业科学院蔬菜花卉研究所,重庆 400055
  • 出版日期:2020-04-25 发布日期:2020-04-25
  • 基金资助:
    国家重点研发计划项目(2016YFD0100204);国家自然科学基金项目(31501756);重庆市自然科学基金项目(cstc2019jcyj-msxm2291);中央高校基本科研业务费专项(XDJK2018B039);重庆市技术创新与应用发展专项(cstc2019jscx-gksbX0149)

Cloning and Functional Analysis of SmDAD1 Promoter in Solanum melongena

ZHANG Shaowei1,*,YUAN Chao1,*,NIU Yi1,*,TANG Qinglin1,WEI Dayong1,WANG Yongqing2,TIAN Shibing2,YANG Yang2,**,and WANG Zhimin1,**   

  1. 1Chongqing Key Laboratory of Olericulture,Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education,College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China;2The Institute of Vegetables and Flowers,Chongqing Academy of Agricultural Sciences,Chongqing 400055,China
  • Online:2020-04-25 Published:2020-04-25

摘要: 以茄子‘F142’为材料,通过染色体步移法克隆了花药开裂相关基因SmDAD1上游746 bp 的启动子序列。通过生物信息在线软件预测,该启动子中含有多个与植物发育及逆境应答相关的顺式作用元件。为进一步分析SmDAD1启动子的功能,构建prSmDAD1::GUS融合表达载体,转化到拟南芥,并对T3代纯合转基因拟南芥进行GUS染色分析。结果表明,SmDAD1启动子在拟南芥幼苗的根部、叶片和花药均有较强活性,其中在根部活性最强,在茎中没有活性。检测转基因植株GUS基因的表达量发现,SmDAD1启动子受茉莉酸甲酯(MeJA)、脱落酸(ABA)、水杨酸(SA)、赤霉素(GA)、生长素(IAA)和1–氨基环丙烷–1–羧酸(ACC)诱导。因此推测SmDAD1可能在茄子发育和抗外界逆境胁迫中起重要作用。

关键词: 茄子, 启动子, SmDAD1, GUS 基因, 功能分析

Abstract: The promoter of SmDAD1 gene with 746 bp was cloned from eggplant accession‘F142’. Bioinformatics online software prediction showed that the promoter contained a plurality of cis-acting elements related to plant development and stress response. In order to further analyze the function of SmDAD1 promoter,prSmDAD1::GUS fusion expression vector was constructed. It was transferred into Arabidopsis thaliana,and GUS staining analysis was performed on T3 generation homozygous transgenic plant. The results showed that SmDAD1 promoter had very high activity in the roots,leaves and anthers,especially in the roots,but no activity in stems. Detection of GUS gene expression in transgenic plants indicated that SmDAD1 promoter was induced by MeJA,ABA,SA,GA,IAA and ACC. Therefore,we speculate that SmDAD1 may play important role in eggplant development and resistance to external stress.

Key words: Solanum melongena, promoter, SmDAD1, GUS gene, functional analysis

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