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园艺学报 ›› 2019, Vol. 46 ›› Issue (11): 2143-2154.doi: 10.16420/j.issn.0513-353x.2019-0076

• 研究论文 • 上一篇    下一篇

CmsCRC与佛手指状结构的伸展相关并受低温激活

陈泽宇1,王玉晶1,徐启越1,韩晓霞2,胡秋梅3,杨 莉1,陈文荣1,廖芳蕾1,*,郭卫东1,*   

  1. 1浙江师范大学化学与生命科学学院,浙江金华 321004;2杭州市求知小学,杭州 310022;3新余市第一中学,江西新余 338000
  • 出版日期:2019-11-25 发布日期:2019-11-25
  • 基金资助:
    浙江省自然科学基金项目(LY17C020001);浙江省科技厅公益性项目(2016C32010);浙江省科技厅重点研发计划项目(2018C02007);浙江省2018年度高校国内访问学者“教师专业发展项目”(FX2018005)

CmsCRC is Involved in Regulated Fingers Stretch in Fingered Citron and Activated Under Lower Temperature

CHEN Zeyu1,WANG Yujing1,XU Qiyue1,HAN Xiaoxia2,HU Qiumei3,YANG Li1,CHEN Wenrong1,LIAO Fanglei1,*,and GUO Weidong1,*   

  1. 1College of Chemistry and Life Science,Zhejiang Normal University,Jinhua,Zhejiang 321004,China;2Hangzhou Qiuzhi Primary School,Hangzhou 310022,China;3Xinyu First Middle School,Xinyu,Jiangxi 338000,China
  • Online:2019-11-25 Published:2019-11-25

摘要: 佛手(Citrus medica L. var. sarcodactylis Swingle)是香橼(C. medica L.)的变种,两者亲缘关系相近但果形差异巨大。青皮佛手一般为指状,如果以较高温度(20 ℃)诱导成花可以得到拳(状)佛手。石蜡切片发现拳佛手的内层心皮在开花前部分融合。利用qRT-PCR技术分析了CRC在拳佛手、指佛手及香橼从花芽到花后的表达,发现指佛手CRC表达量显著低于香橼和拳佛手,且CRC在拳佛手花器官的表达模式与香橼类似,表明果形的变化可能与CRC的差异表达有关。在香橼和指佛手中分离了CRC基因的全长,发现指佛手CRC启动子较香橼缺失了两个TATA-Box元件。构建指佛手和香橼部分CRC启动子的瞬时表达载体转化本氏烟,常温下指佛手CmsCRC启动子活性低于香橼。低温(4 ℃)诱导后,香橼和指佛手CRC启动子活性增强,且指佛手的活性强于香橼,表明相对于香橼,指佛手启动子在低温下更加敏感。因此,CmsCRC可能与佛手指状结构的伸展相关且受低温的诱导。

关键词: 佛手, CmsCRC, 果形, 启动子活性, 低温, 心皮融合

Abstract: Fingered citron(Citrus medica L. var. sarcodactylis Swingle),a variant of citron(C. medica L.),has a close genetic relation with citron while huge difference in fruit shape. The fingers of ‘Qingpi’,a fingered citron,commonly stretch out,but rolled up to form a fist when cultured under 20 ℃ during flower formation. Paraffin sections revealed that the inner carpel of the fist-like citron partially fused before anthesis. qRT-PCR technology was used to analyze the expression patterns of CRC in fingered citron,fist-like fingered citron,and the citron during the entire stages of flower development. The result shows that the expression pattern of CRC in fingered citron was significantly lower the other two. In addition,the expression pattern of fist-like fingered citron was quite close to that of the citron. It implied that the fruit shape difference is associated with the CRC gene expression patterns. The full length of CRC gene was identified from fingered citron and citron. Compared with citron,the promoter of CRC in fingered citron lacked two TATA-Box elements. Both CRC promoters in fingered citron and citron were transiently transformed into N. benthamiana leaves. It shows that the activity of CRC promoter from the fingered citron was lower than that from the citron under room temperature. When cultured under low temperature(4 ℃),CRC promoters activities were enhanced either for the fingered citron or the citron. However,the promoter activity in the fingered citron was much more stronger than that in the citron. It indicates that the promoter of fingered citron is more sensitive to low temperature than that of citron. Therefore,CmsCRC maybe involved in regulating the fingers stretch in fingered citron and activated by lower temperature.

Key words: fingered citron, CmsCRC, fruit shape, promoter activity, low temperature, carpel fusion

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