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园艺学报 ›› 2019, Vol. 46 ›› Issue (5): 901-909.doi: 10.16420/j.issn.0513-353x.2018-0659

• 研究论文 • 上一篇    下一篇

一氧化氮通过调控水分和抗氧化酶活性参与脱落酸延缓切花月季衰老

方 华,王春蕾,廖伟彪*,张 静,霍建强,黄登静,牛丽涓,王 波   

  1. 甘肃农业大学园艺学院,兰州 730070
  • 出版日期:2019-05-25 发布日期:2019-05-25
  • 基金资助:
    国家重点研发计划项目(2018YFD1000800);国家自然科学基金项目(31860568,31560563,31160398);中国博士后科学基金项目(20100470887,2012T50828);甘肃省自然科学基金项目(1606RJZA073,1606RJZA077)

NO is Involved in ABA-regulated Senescence of Cut Roses by Maintaining Water Content and Increasing Antioxidant Enzyme Activities

FANG Hua,WANG Chunlei,LIAO Weibiao*,ZHANG Jing,HUO Jianqiang,HUANG Dengjing,NIU Lijuan,and WANG Bo   

  1. College of Horticulture,Gansu Agricultural University,Lanzhou 730070,China
  • Online:2019-05-25 Published:2019-05-25

摘要: 以切花月季‘坦尼克’(Rosa hybrida‘Tineke’)为材料,研究了脱落酸(ABA)和一氧化氮(NO)对其衰老的影响及其相互关系。结果表明:0.5 μmol · L-1 ABA溶液瓶插处理的切花月季瓶插寿命和花径明显高于蒸馏水处理(对照),而1.0和2.0 μmol · L-1 ABA处理的花径显著小于对照。150 μmol · L-1 NO供体硝普钠(SNP)溶液瓶插处理的瓶插寿命明显高于对照,比对照高70.7%。NO合成抑制剂钨酸钠(Na2WO4)抑制了ABA对瓶插寿命和花径的促进作用,说明ABA延缓切花衰老需要NO的参与。进一步分析表明,ABA处理保持了切花月季叶片的相对含水量,而NO合成抑制剂钨酸钠抑制了ABA对含水量的保持。此外,ABA处理明显提高了切花月季超氧化物歧化酶、过氧化物酶和抗坏血酸过氧化物酶的活性,相反,钨酸钠抑制了ABA这一作用,表明ABA提高切花月季抗氧化能力需NO的参与。综上,NO参与了ABA通过保持水分和提高抗氧化酶活性来延缓切花月季衰老的过程。

关键词: 月季, 切花, 一氧化氮, 脱落酸, 含水量, 抗氧化酶

Abstract: Cut roses(Rosa hybrida‘Tineke’)were used to investigate the effects of abscisic acid (ABA)and nitric oxide(NO)on cut flower senescence and the interaction between NO and ABA during postharvest freshness. The results show that 0.5 μmol · L-1 ABA significantly increased the vase life and flower diameter compared with distilled water(the control). However,1.0 and 2.0 μmol · L-1 ABA significantly reduced flower diameter in comparison with the control. 150 μmol · L-1 NO donor SNP increased vase life by 70.7% compared with the control. NO synthesis inhibitor sodium tungstate significantly inhibited the promotive effects of ABA on vase life and flower diameter,showing that NO was required for ABA-regulated senescence of cut roses. Moreover,ABA kept the relative water content of cut rose leaves,while sodium tungstate inhibited the positive effects of ABA. Furthermore,ABA significantly increased the activities of superoxide dismutase,peroxidase and ascorbate peroxidase in cut rose leaves. However,the promotive effect of ABA on the activities of antioxidant enzymes was reversed by sodium tungstate,indicating that NO is involved in ABA-induced antioxidant capacity during postharvest freshness. Together,our results show that NO might be involved in ABA-delayed senescence of cut flowers by maintaining water content and increasing activities of antioxidant enzymes.

Key words: rose, cut roses, nitric oxide, abscisic acid, water content, antioxidant enzyme

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