Abstract: cDNA of Pol cytop lasmic male sterility (CMS) and its maintainer line were amp lified with 30
pairs of SRAP ( sequence related amp lified polymorphism) p rimers combination, and two fragmentsBcCMS1 and
BcCMS2 were obtained. Information from cloning and sequencing of these two bands indicated thatBcCMS1 was
of 63% of homology with 19 amino acids of chromosome related to cytogenetic of B rassica rapa, BcCMS2 was of
88% of homologywith the FLC3 gene for inhibiting flower gene of Brassica oleracea. The accession numbers for
these two sequences in GenBank were DQ914921 and DQ914922, respectively. The results obtained suggested
that the exp ression of the nuclear gene in cytogenetic male sterility was affected by the cytop lasmic gene. The
SRAP technique was firstly successfully adop ted in the research of gene differential exp ression.

Key words: Brassica rapa ssp. chinensis, CMS, SRAP, Geneexp ression