Detection of Strawberry mottle virus and Strawberry mild yellow edge virus byMultiplex RT-PCR

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (3): 507-510.

• 研究论文 • Previous Articles Next Articles

Detection of Strawberry mottle virus and Strawberry mild yellow edge virus byMultiplex RT-PCR

Zhang Zhihong;Yang Hongyi^*;Dai Hongyan; Li He;Xiao Min

(College of Horticulture, Shenyang Agricultural University, Shenyang, L iaoning 110161, China)

Received:2005-05-20 Revised:2005-07-01 Online:2006-06-25 Published:2006-06-25

Abstract

Abstract: Amultiplex reverse transcriptase polymerase chain reaction (RT - PCR) assay was developed for simultaneously detecting Strawberry mottle virus ( SMoV) and Strawberry mild yellow edge virus ( SMY2EV). Both field-grown strawberries and microplants were detected effectively. The primers of multiplex PCR were selected by primer-primer interactions and melting temperature. In multiplex PCR for detecting SMoV and SMYEV, the appropriate concentration of PCR buffer was 2 ×, and the appropriate temperatures for anneal and extension were 57℃ and 66℃ respectively. Eleven lines of strawberry ( Fragaria ×ananassa) cultivar‘Hokowase’, which were obtained by mini shoot tip culture in vitro, were screened for virus elimination by standard RT - PCR and multiplex RT - PCR.

Key words: Multiplex RT - PCR, Virus detection, Strawberry

Zhang Zhihong;Yang Hongyi;Dai Hongyan;Li He;Xiao Min. Detection of Strawberry mottle virus and Strawberry mild yellow edge virus byMultiplex RT-PCR[J]. ACTA HORTICULTURAE SINICA, 2006, 33(3): 507-510.

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Detection of Strawberry mottle virus and Strawberry mild yellow edge virus byMultiplex RT-PCR

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