Abstract: Seven kinds of F1 generation cucumber unpollinated ovary were used as test materials for regenerated plant cultivation. Paraffin sections of ovary were observed，and hormone content of ovary slices at different culture stages were measured to explore the formation process and related physiological changes of embryonary sac regenerated plants. The results showed that the regenerated plants were obtained from unpollinated ovary culture of cucumbers with three donor materials under MS + 0.06mg · L-1 TDZ medium，which were identified as haploid，double haploid and autotetraploid respectively by flow cytometry，chromosome count and SSR. A large number of embryonary like structures were produced in ovary under MS + 0.04 mg · L-1 TDZ + 1.0 mg · L-1 6-BA + 0.1 mg · L-1 NAA + 12 mg · L-1 AgNO3medium，but no regenerated plants were obtained after differentiation culture. The embryonary sac underMS and MS + 0.06 mg · L-1 TDZ medium was observed to develop on the 5th and 17th day by paraffin sections. Hormone determination suggested that the content of GA and IAA are on the decline to the lowest at the 8th day and increased subsequently. The GA content in the ovary slices at 17 d was higher than that in the culture for 2 d，but the variation of IAA content is slight.

Key words: cucumber, ovary culture, embryonary sac regenerated plant