An Improved Method for Extraction and Identification of Potato RNAs

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (4): 1059-1062.

• 新技术与方法 • Previous Articles Next Articles

An Improved Method for Extraction and Identification of Potato RNAs

BAI Yun-feng; GUO Zhi-hua; BAI Dong-mei; WANG Xiao-qi; ZHANGWei-feng^*

(Crop Genetics Institute, Shanx i Academ y of Agricultural Sciences, Taiyuan 030031,China)

Received:2007-01-15 Revised:2007-04-28 Online:2007-08-25 Published:2007-08-25

Abstract

Abstract: An improved method of extracting and identifying total RNAs from potato leaf and stem was put forward. The electrophoretic patterns and absorbance at 260 nm and 280 nm in a UV-Vis spectrophotometer revealed that the total RNAs of potato extracted by this method has good completeness and high purity. Furthermore, the result of RT-PCR indicated that the RNAs prepared with this improved method could meet the needs of most molecular biological experiment including gene cloning and expression analysis.

Key words: Potato, Total RNAs extraction

BAI Yun-feng;GUO Zhi-hua;BAI Dong-mei;WANG Xiao-qi;ZHANGWei-feng. An Improved Method for Extraction and Identification of Potato RNAs[J]. ACTA HORTICULTURAE SINICA, 2007, 34(4): 1059-1062.

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An Improved Method for Extraction and Identification of Potato RNAs

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