Polyploidy Induction and Identification of Lilium pumilum and Lilium davidii var. unicolor Based on Somatic Embryogenesis

doi:10.16420/j.issn.0513-353x.2017-0879

ACTA HORTICULTURAE SINICA ›› 2018, Vol. 45 ›› Issue (6): 1136-1146.doi: 10.16420/j.issn.0513-353x.2017-0879

• Research Papers • Previous Articles Next Articles

Polyploidy Induction and Identification of Lilium pumilum and Lilium davidii var. unicolor Based on Somatic Embryogenesis

SUN Hongmei1,2,*，FU Linlan1，WANG Zhiping1，GAI Meizhu1，and WANG Chunxia1,2

1College of Horticulture，Shenyang Agricultural University，Shenyang 110866，China；2Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province，Shenyang 110866，China

Online:2018-06-25 Published:2018-06-25

Abstract

Abstract: Using scales and embryogenic callus as the target tissues，Lilium pumilum DC. Fisch. and Lilium davidii var. unicolor were accomplished the ployploid inducement by soaking and mixed culture with different concentrations of colchicine. Nineteen tetraploids of Lilium pumilum DC. Fisch. and 6 tetraploids of Lilium davidii var. unicolor have been identified，through morphological observation，root tip chromosome count and stomatal observation. The optimum condition is immersing embryogenic callus with 0.1% colchicine for 24 hours to achieve the highest efficiency of chromosome doubling. Dipold plants of both lilies are genetically stable while polyploid plants experience the genetic variation by using ISSR to conduct the genetic analysis of plants via somatic embryogenesis. The variation rates of polyploid in Lilium pumilum DC. Fisch. and Lilium davidii var. unicolor are 15.48% and 9.75%，respectively.

Key words: Lilium pumilum, Lilium davidii var. unicolor, somatic embryogenesis, polyploid, ISSR

CLC Number:

S 682.2

SUN Hongmei1,2,*，FU Linlan1，WANG Zhiping1，GAI Meizhu1，and WANG Chunxia1,2. Polyploidy Induction and Identification of Lilium pumilum and Lilium davidii var. unicolor Based on Somatic Embryogenesis[J]. ACTA HORTICULTURAE SINICA, 2018, 45(6): 1136-1146.

[1]	ZHU Wei, CAO Jinjin, CHEN Xi, ZHANG Wei, SUN Rongze, ZHU Shaocai, ZHAO Jiageng, CUI Yaqi, WANG Yuxuan, and YU Xiaonan. New Herbaceous Peony Cultivars‘Fluttering Pink’‘Fairy’s Cheek’ ‘Blushing Smile’and‘Tiny Lotus’ [J]. Acta Horticulturae Sinica, 2023, 50(2): 457-458.
[2]	JIANG Jingdong, WEI Zhuangmin, WANG Nan, ZHU Chenqiao, YE Junli, XIE Zongzhou, DENG Xiuxin, CHAI Lijun. Exploitation and Identification of Tetraploid Resources of Hongkong Kumquat（Fortunella hindsii） [J]. Acta Horticulturae Sinica, 2023, 50(1): 27-35.
[3]	XU Xiaoping, CAO Qingying, CAI Roudi, GUAN Qingxu, ZHANG Zihao, CHEN Yukun, XU HAN, LIN Yuling, LAI Zhongxiong. Gene Cloning and Expression Analysis of miR408 and Its Target DlLAC12 in Globular Embryo Development and Abiotic Stress in Dimocarpus longan [J]. Acta Horticulturae Sinica, 2022, 49(9): 1866-1882.
[4]	WU Ting, JIA Ruidong, YANG Shuhua, ZHAO Xin, YU Xiaonan, GUO Yuan, GE Hong. Research Advances and Prospects on Phalaenopsis Polyploid Breeding [J]. Acta Horticulturae Sinica, 2022, 49(2): 448-462.
[5]	SHEN Xu, CHEN Xiaohui, ZHANG Jing, CHEN Rongzhu, XU Xiaoping, LI Xiaofei, JIANG Mengqi, LIU Pudong, NI Shanshan, LIN Yuling, LAI Zhongxiong. Evolutionary Dynamics Investigation and the Expression Analysis of Chromatin Remodeling Factor Snf2 Gene Family During Early Somatic Embryogenesis in Dimocarpus longan [J]. Acta Horticulturae Sinica, 2022, 49(1): 41-61.
[6]	ZHANG Chunyu, XU Xiaoqiong, XU Xiaoping, ZHAO Pengcheng, SHEN Xu, Munir Nigarish, ZHANG Zihao, LIN Yuling, Chen Zhenguang, LAI Zhongxiong. Genome-wide Identification of the SKP1-like Family and Analysis of Their Expression During Early Somatic Embryogenesis in Longan [J]. Acta Horticulturae Sinica, 2021, 48(9): 1665-1679.
[7]	WANG Xin, LI Mingyang, TIAN Lin, LIU Dongyun. ISSR and rDNA-ITS Sequence Analysis of the Genetic Relationship of Clematis in Hebei Province [J]. Acta Horticulturae Sinica, 2021, 48(9): 1755-1767.
[8]	YU Bo1，HUANG Lili1，ZHU Yu2，ZHU Genfa1，and SUN Yingbo1,*. Induction of Embryogenic Calli from Immature Pedicels and Efficient Plant Regeneration of Hippeastrum [J]. ACTA HORTICULTURAE SINICA, 2020, 47(5): 907-915.
[9]	YA Rong1，XU Weirong2,3,4,5,*，ZHANG Ying1，XIA Siqi2，and ZHANG Ningbo2,3,5. Investigation of Melatonin on Somatic Embryo Induction for‘Thompson Seedless’Grapevine [J]. ACTA HORTICULTURAE SINICA, 2020, 47(5): 953-962.
[10]	ZHOU Rui, XIE Kaidong, WANG Wei, PENG Jun, XIE Shanpeng, HU Yibo, WU Xiaomeng, and GUO Wenwu, . Efficient Identification of Tetraploid Plants from Seedling Populations of Apomictic Citrus Genotypes Based on Morphological Characteristics [J]. Acta Horticulturae Sinica, 2020, 47(12): 2451-2458.
[11]	DANG Jiangbo1，LIANG Guolu1，LI Cai2，WU Di1，GUO Qigao1,*，LIANG Senlin1，and WANG Peng1. Polyploid Rootstock of Fruit Tree：Research Status and Prospects [J]. ACTA HORTICULTURAE SINICA, 2019, 46(9): 1701-1710.
[12]	BEN Haiyan1,3，QU Hongyun1，GAO Wei3，ZHANG Junmin1，ZHANG Xueyan1，LI Baoju2,，and CHAI Ali2,. Pathogenicity Differentiation and ISSR Genetic Variation Analysis of Verticillium dahliae Isolates from Eggplant in Northeast China [J]. ACTA HORTICULTURAE SINICA, 2019, 46(7): 1320-1332.
[13]	WANG Chunxia，YIN Yue，WANG Zhiping，YAN Rui，Fu Linlan，and SUN Hongmei*. Effects of Regeneration and Abiotic Stress Tolerance in Polyploid Lilium davidii var. unicolor and Lilium pumilum in Vitro [J]. ACTA HORTICULTURAE SINICA, 2019, 46(12): 2359-2368.
[14]	LUAN Aiping1，HE Junhu1,，LIU Jiarou2，GONG Xue2，FANG Shaoqiu2，XIA Jingxian2，LIN Wenqiu3，XU Wentian3，and HE Yehua2,. Character Identification of the Short-vegetative-growth-period Mutant ‘14-1’in Ananas comosus var. nanus [J]. ACTA HORTICULTURAE SINICA, 2019, 46(1): 143-148.
[15]	JIANG Yawen，SUN Xiaoqin，LUO Huolin，YANG Boyun，and XIONG Dongjin. Studies on Genetic Diversity of Cymbidium kanran Populations from the Main Mountains in Jiangxi Province Based on ISSR Marker [J]. ACTA HORTICULTURAE SINICA, 2017, 44(10): 1993-2001.

Polyploidy Induction and Identification of Lilium pumilum and Lilium davidii var. unicolor Based on Somatic Embryogenesis

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments