Abstract: The watermelon germplasm P.I.595203 resistant to ZYMV-CH ( Zucchini Yellow Mosaic Virus-CHINA ) and 98R susceptible to ZYMV-CH were used as the parents to construct F₂ population and F_2:3 population. The markers linked to the resistant gene zym-CH were filtered by BSA strategy.The reference genetic map constructed basing on the F₂S₈ population（P.I.296341-FR×97103）was used for comparative mapping of zym-CH gene. The results showed that b13-₁₄₀₀, MCPI-₁₄ and MCPI-₂₆ were linked to zym-CH gene with the genetic distance of 25.6 cM, 57cM and 87cM respectively. The resistant gene zym-CH was located on 105 cM of the linkage group 3 by the collinearity relationship of zym-CH, b13-1400, MCPI-₁₄ and MCPI-₂₆ in the two linkage maps.

Key words: watermelon, the resistant gene for zucchini yellow mosaic virus, bulked segregant analysis, collinearity relationship, comparative mapping