Application of AFLP Markers for Identification of Hybrids from Open Pollinated Dongzao (Zizyphus jujuba Mill. ) Progenies

ACTA HORTICULTURAE SINICA ›› 2005, Vol. 32 ›› Issue (4): 680-684.

• 研究报告 • Previous Articles Next Articles

Application of AFLP Markers for Identification of Hybrids from Open Pollinated Dongzao (Zizyphus jujuba Mill. ) Progenies

Lu Jinying; Mao Yongmin;Shen Lianying;Peng Shiqi;Liu Min

(1 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; 2 Research Center of Chinese Jujube, Agricultural University of Hebei, Baoding 071001, China)

Received:2005-02-01 Revised:2005-06-06 Online:2005-08-25 Published:2005-08-25

Abstract

Abstract: The 65 open-pollinated ‘Dongzao’ ( Zizyphus jujuba Mill. ) progenies were detected with AFLP technique. Twelve pairs of primers which amplified distinct and polymorphic segments were selected from 81 pairs of EcoRⅠand MseⅠ primers. Then twelve pairs of primers were used in the AFLP analysis. Altogether 517 bands were p roduced among which 376 bands were polymorphic ones with the percentage of polymorphism being 72.7%. Ten markers were present only in ‘Jinsi Xiaozao’. Thirty-four hybrids from
‘Dongzao’×‘Jinsi Xiaozao’were identificated. Seven markers were present only in ‘Jianzao’. Fifteen hybrids from ‘Dongzao’×‘J ianzao’were identificated. Sixteen plants were not confirmed.

Key words: Chinese jujube, Progeny, AFLP marker, Identification of hybrid

CLC Number:

S 665.1

Lu Jinying;Mao Yongmin;Shen Lianying;Peng Shiqi;Liu Min. Application of AFLP Markers for Identification of Hybrids from Open Pollinated Dongzao (Zizyphus jujuba Mill. ) Progenies[J]. ACTA HORTICULTURAE SINICA, 2005, 32(4): 680-684.

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Application of AFLP Markers for Identification of Hybrids from Open Pollinated Dongzao (Zizyphus jujuba Mill. ) Progenies

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