Comparison of Amplification Effects on Apricot (Prunus armeniaca L.) Resources in China Using Different Primer Combinations

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (5): 1141-1146.

• 果树 • Previous Articles Next Articles

Comparison of Amplification Effects on Apricot (Prunus armeniaca L.) Resources in China Using Different Primer Combinations

ZHANGLi-jie; CHEN Xue-sen^*;CHEN Xiao-liu;FENG Jian-rong; LIU Xiao-li;CI Zhi-juan

(State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai^,an, Shandong, 271018, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-10-25 Published:2007-10-25

Abstract

Abstract: S genotypes of 30 apricot cultivars native to China were detected usingS-allele-specific PCR with 5 pairs of Prunus consensus primer combinations which had been published, and amplification effects were evaluated according to coefficient and efficiency of amplification. The results were as follows: ①The effects of PCR amplification with 5 primer combinations were different greatly. The primer combination EM-PC2consFD+EM-PC3consRD was the most suitable for Chinese apricot, and its’ coefficient and efficiency of amplification were 66.7% and 53.3%, respectively; ②No amplification was obtained using the combination EM-PC2consFD+EM-PC3consRD in the two cultivars ‘Huaxian Dajiexing’ and ‘Zhupi Shuixing’ while the other two combinations, PruC2+PruC4R and PaConsⅡ-F+PaConsⅡ-R, could amplify two fragments: In addition, no amplification was obtained using all the five pairs of combinations in the two cultivars ‘Lanzhou Dajiexing’ and ‘Baimuxing’. Then it could be concluded that, to detect S genotypes of all Chinese apricot cultivars accurately, new specific primers need to be developed besides the five primer combinations; ③The results amplified in the four cultivars ‘Hongfeng’,‘Xinshiji’, ‘Honghebao’ and ‘Erhuacao’ by two pairs of combinations, EM-PC2consFD+EM-PC3consRD and PruC2+PruC4R, showed that it was of validity to evaluate amplification effects of different primer combinations according to coefficient and efficiency of amplification in this study.

Key words: Apricot, S-gene, Primer combination, Amplification effect

ZHANGLi-jie;CHEN Xue-sen;CHEN Xiao-liu;FENG Jian-rong;LIU Xiao-li;CI Zhi-juan. Comparison of Amplification Effects on Apricot (Prunus armeniaca L.) Resources in China Using Different Primer Combinations[J]. ACTA HORTICULTURAE SINICA, 2007, 34(5): 1141-1146.

[1]	YUAN Kejun, NIU Qinglin, QIN Zhihua, and WANG Peijiu. A New Early-ripening Apricot Cultivar‘Yinghua’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 27-28.
[2]	GE Furong, YUAN Kejun, NIU Qinglin, YANG Yuliang, ZHUANG Di, and XU Ping. A New Early-ripening Apricot Cultivar‘Liyuan’ [J]. Acta Horticulturae Sinica, 2020, 47(S2): 2887-2888.
[3]	WANG Shaomin, RAN Kun, WEI Shuwei, WANG Hongwei, ZHANG Yong, DONG Ran, and DONG Xiaochang. A New Early-maturing Apricot Cultivar‘Luxing 6’ [J]. Acta Horticulturae Sinica, 2020, 47(S2): 2889-2890.
[4]	FENG Jing1，YANG Can1，LU Juanfang1，and XI Wanpeng1,2,*. Cloning and cis-acting Element Analysis of CCD1 and CCD4 Promoter in Apricot [J]. ACTA HORTICULTURAE SINICA, 2020, 47(5): 939-952.
[5]	CHEN Yahan1,2,，MA Qiang1,，SUN Pingping1，ZHANG Lei1，and LI Zhengnan1,**. Identification of Viruses Causing Apricot Decline and Leaf Chlorosis Disease by siRNA High-throughput Sequencing and RT-PCR Detection [J]. ACTA HORTICULTURAE SINICA, 2020, 47(4): 725-733.
[6]	SUN Haoyuan，YANG Li，ZHANG Junhuan，JIANG Fengchao，ZHANG Meiling，and WANG Yuzhu*. A New Apricot Cultivar‘Jingluofeng’ [J]. ACTA HORTICULTURAE SINICA, 2020, 47(4): 805-806.
[7]	YUAN Kejun, WANG Peijiu, NIU Qinglin, GE Furong, and LI Shenglong. A New Mid-ripening and Big-fruit Apricot Cultivar‘Xiahua’ [J]. Acta Horticulturae Sinica, 2020, 47(10): 2063-2064.
[8]	YUAN Kejun1,*，WANG Peijiu1，NIU Qinglin1，GE Furong1，and LI Shenglong2. A New Early- or Medium-ripening Apricot Cultivar‘Yuhua’ [J]. ACTA HORTICULTURAE SINICA, 2019, 46(S2): 2743-2744.
[9]	YUAN Kejun1,*，WANG Peijiu1，LI Shenglong2，NIU Qinglin1，GE Furong1，MENG Xiaoye3，and DAI Huafeng4. A New Very Early-ripening Apricot Cultivar‘Chunhua’ [J]. ACTA HORTICULTURAE SINICA, 2019, 46(S2): 2745-2746.
[10]	YANG Li，SUN Haoyuan，ZHANG Junhuan，JIANG Fengchao，ZHANG Meiling，and WANG Yuzhu*. A New Apricot Cultivar‘Jingluohong’ [J]. ACTA HORTICULTURAE SINICA, 2019, 46(9): 1853-1854.
[11]	LIU Shuo1,2，LIU Ning1，ZHANG Qiuping1，ZHANG Yuping1，ZHANG Yujun1，XU Ming1，MA Xiaoxue1，and LIU Weisheng1,*. Genetic Diversity of the Apricot Germplasms from North & Northeast China [J]. ACTA HORTICULTURAE SINICA, 2019, 46(6): 1045-1056.
[12]	WANG Shaomin*，RAN Kun，WEI Shuwei，WANG Hongwei，ZHANG Yong，DONG Ran，and DONG Xiaochang. A New Early-maturing Apricot Cultivar‘Luxing 4’ [J]. ACTA HORTICULTURAE SINICA, 2018, 45(S2): 2717-2718.
[13]	ZHANG Qiuping and LIU Weisheng*. Advances of the Apricot Resources Collection，Evaluation and Germplasm Enhancement [J]. ACTA HORTICULTURAE SINICA, 2018, 45(9): 1642-1660.
[14]	ZHAO Xiping1，WU Xiaohong1,*，ZHANG Xiancheng2，YUAN Liyong3，LI Liying4，TANG Huanying3，and CUI Qizhi4. A New Very Early-maturing Apricot Cultivar‘Jinhe’ [J]. ACTA HORTICULTURAE SINICA, 2018, 45(7): 1417-1418.
[15]	LIU Shengyu1，ZHANG Shikui2，LU Juanfang1，LI Wenhui2，and XI Wanpeng1,3,*. Molecular Cloning and Function Analysis of Carotenoid Cleavage Dioxygenase 1（CCD1）in Apricot Fruit [J]. ACTA HORTICULTURAE SINICA, 2018, 45(3): 471-482.

Comparison of Amplification Effects on Apricot (Prunus armeniaca L.) Resources in China Using Different Primer Combinations

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments