Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (9): 2183-2194.doi: 10.16420/j.issn.0513-353x.2023-0564
• Plant Protection • Previous Articles Next Articles
CHEN Ming, ZHANG Jieru, YANG Hangyun, WANG Yinbao, ZHENG Zhiyuan, ZENG Jiaoke, CHEN Jinyin, FU Yongqi*(), XIANG Miaolian*(
)
Received:
2024-01-02
Revised:
2024-06-21
Online:
2024-09-25
Published:
2024-09-19
Contact:
FU Yongqi, XIANG Miaolian
CHEN Ming, ZHANG Jieru, YANG Hangyun, WANG Yinbao, ZHENG Zhiyuan, ZENG Jiaoke, CHEN Jinyin, FU Yongqi, XIANG Miaolian. Induction Mechanism of Methyl Jasmonate on Postharvest Navel Orange Fruit Against Blue Mold[J]. Acta Horticulturae Sinica, 2024, 51(9): 2183-2194.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2023-0564
甜橙基因 Orange gene | 引物序列(5′-3′) Primer sequence | 片段大小/bp Size | NCBI号 NCBI No. |
---|---|---|---|
CsPOD | F-GACATTCGCTATGCCGTTCT;R-TCGGTCCCGTTGTAATCG | 94 | XM_006492612.2 |
CsPPO | F-ATGAGATGCCAATAGGGCCG;R-AATGCGAGCTAGACCCTTCC | 253 | XM_006468155.2 |
CsPAL | F-GATTACGGATTCAAGGGTGC;R-TTGGTGACAGGTATTGGCGAG | 80 | XM_006485585.3 |
CsCAD | F-CCTTTGGTGCCTGGACATGAG;R-GTGTAAACTGACCGAGCACAA | 170 | XM_006476176.3 |
Cs4CL | F-ACCGGCGACATTGGTTACAT;R-TGGAGTGCGAGAGAAGCAAG | 130 | XM_006494749.3 |
CsC4H | F-GTACTTGCCTTTTGGCGTGG;R-TGCAATCACAAGCCCCAAGA | 79 | NM_001288840.1 |
CsLAC | F-CGCAATGTCCAATTCAGCC;R-GCTTTCCACCATTCACCGA | 208 | XM_006486310.3 |
CsActin | F-CATCCCTCAGCACCTTCC;R-CCAACCTTAGCACTTCTCC | 195 | XM_006464503.3 |
Table 1 Primers for qRT-PCR analysis
甜橙基因 Orange gene | 引物序列(5′-3′) Primer sequence | 片段大小/bp Size | NCBI号 NCBI No. |
---|---|---|---|
CsPOD | F-GACATTCGCTATGCCGTTCT;R-TCGGTCCCGTTGTAATCG | 94 | XM_006492612.2 |
CsPPO | F-ATGAGATGCCAATAGGGCCG;R-AATGCGAGCTAGACCCTTCC | 253 | XM_006468155.2 |
CsPAL | F-GATTACGGATTCAAGGGTGC;R-TTGGTGACAGGTATTGGCGAG | 80 | XM_006485585.3 |
CsCAD | F-CCTTTGGTGCCTGGACATGAG;R-GTGTAAACTGACCGAGCACAA | 170 | XM_006476176.3 |
Cs4CL | F-ACCGGCGACATTGGTTACAT;R-TGGAGTGCGAGAGAAGCAAG | 130 | XM_006494749.3 |
CsC4H | F-GTACTTGCCTTTTGGCGTGG;R-TGCAATCACAAGCCCCAAGA | 79 | NM_001288840.1 |
CsLAC | F-CGCAATGTCCAATTCAGCC;R-GCTTTCCACCATTCACCGA | 208 | XM_006486310.3 |
CsActin | F-CATCCCTCAGCACCTTCC;R-CCAACCTTAGCACTTCTCC | 195 | XM_006464503.3 |
Fig. 1 Effects of methyl jasmonate(MeJA)and its synthesis inhibitor(SHAM)pretreatments on the disease resistance of navel orange inoculated with Penicillium italicum The different lowercase letters indicate significant differences among treatments at 0.05 level. The same below.
Fig. 2 Effects of methyl jasmonate(MeJA)and its synthesis inhibitor(SHAM)on the activities of related enzyme. in navel orange inoculated with Penicillium italicum * indicates significant differences between MeJA treatment and the control(P < 0.05),** indicate extremely significant differences between MeJA treatment and the control(P < 0.01). The same below.
Fig. 3 Effects of methyl jasmonate(MeJA)and its synthesis inhibitor(SHAM)pretreatments on phenylpropane metabolizing enzyme genes expression in navel orange inoculated with Penicillium italicum
Fig. 4 Effects of methyl jasmonate(MeJA)and its synthesis inhibitor(SHAM)pretreatments on the content of lignin etc. in navel orange inoculated with Penicillium italicum
Fig. 5 The sketch map for effects of methyl jasmonate(MeJA)treatment on lignin biosynthesis pathway in navel orange fruits The white box indicates no detection,the red box indicates a significant increase in content,and the blue box indicates a significant decrease in content. The dotted arrow indicates multiple catalytic steps and intermediate products,and the solid arrow indicates further catalysis;the upward red arrow indicates upregulation;the question mark indicates unknown.
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