Cytological Observation of Fruit Peduncle Abscission Zone and Preliminary Mapping of Mature Fruit Abscission AL3 gene in Melon

doi:10.16420/j.issn.0513-353x.2020-1041

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (2): 341-351.doi: 10.16420/j.issn.0513-353x.2020-1041

• Research Papers • Previous Articles Next Articles

Cytological Observation of Fruit Peduncle Abscission Zone and Preliminary Mapping of Mature Fruit Abscission AL3 gene in Melon

SHENG Yunyan¹, YANG Limin¹, DAI Dongyang¹, ZHANG Jiaxin¹, WANG Ling¹, WANG Di², CAI Yi¹, TIAN Limei¹^,^*()

¹College of Horticulture and Landscape Architecture,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163319,China
²Daqing Branch of Heilongjiang Academy of Agricultural Sciences,Daqing,Heilongjiang 163310,China

Received:2021-05-11 Revised:2021-11-03 Online:2022-02-25 Published:2022-02-28
Contact: TIAN Limei E-mail:tianmeili2007@163.com

Abstract

Abstract:

The F₂,F₃,F₄and backcross populations derived from a cross between M2-10 （none-mature fruit abscission,NMFA）and ZT091（mature fruit abscission,MFA）was used to study the cytological observation of abscission zone and gene mapping of one of mature fruit abscission（MFA）gene（AL3）.The analysis of genetic inheritance indicated that the MFA was controlled by two genes. Paraffin section and scanning electron microscopy observation of the parent fruit at the maturity stage showed that the cells in the abscission zone of peduncle of ZT091 were larger in size,loosen arrangement and larger spaces between cells,there were significant difference between parental peduncle abscission zone. AL3 was primary mapped into a 0.355 Mb region（from 10 686 821 to 11 042 074 bp）on chromosome 8 with the strategy of SLAF-BSA using 83-F₃ family only segregated with AL3. F₄family and CAPs markers were used for narrowing down the mapping region. Finally, AL3 was fined mapped into a 64.7 kb（from 10 774 778 to 10 839 486 bp）region with 10 annotated candidate genes.

Key words: melon, fruit abscission, cyotological observation, gene mapping

CLC Number:

S652

SHENG Yunyan, YANG Limin, DAI Dongyang, ZHANG Jiaxin, WANG Ling, WANG Di, CAI Yi, TIAN Limei. Cytological Observation of Fruit Peduncle Abscission Zone and Preliminary Mapping of Mature Fruit Abscission AL3 gene in Melon[J]. Acta Horticulturae Sinica, 2022, 49(2): 341-351.

Figures/Tables 7

References 35

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引物编号 Marker ID	染色体 Chr.	基因组位点 Position	正向引物序列（5′-3′） Forward sequence	反向引物序列（5′-3′） Reverse sequence	酶 Enzyme	酶切位点 Recognition site
SSR20531	8	9 968 964	GCAGACCAAAAATGGGAAAA	GGAATGGATGCAGTGATGC
SNP269	8	10 698 869	GCCAAACGGGTTATGAGGTA	TATTCCATGATTGGACGCCT	AluⅠ	AGCT
SNP361	8	10 761 364	TACCACCGGTTCCATGTTTT	ATGGTGGTTCCAATGACGTT	AluⅠ	AGCT
SNP392	8	10 774 778	TGCATAATACTCATTCAACGCA	TTGATGCTTACTTTGGTGATGC	AluⅠ	AGCT
SNP485	8	10 839 486	TGGAAAAATGGCTTCAAATCT	TGCAAACGATTCGTCATTTC	AluⅠ	AGCT
SNP523	8	10 857 497	CATGGCGCTATGAGGTTTTT	TGCAAAATTTTCTTCCAATCG	MseⅠ	TTAA
SNP700	8	10 934 492	TGGAATGCAAACAAATGTGAA	GGACATCCGGTGTTGAACTT	AluⅠ	AGCT

引物编号 Marker ID	染色体 Chr.	基因组位点 Position	正向引物序列（5′-3′） Forward sequence	反向引物序列（5′-3′） Reverse sequence	酶 Enzyme	酶切位点 Recognition site
SSR20531	8	9 968 964	GCAGACCAAAAATGGGAAAA	GGAATGGATGCAGTGATGC
SNP269	8	10 698 869	GCCAAACGGGTTATGAGGTA	TATTCCATGATTGGACGCCT	AluⅠ	AGCT
SNP361	8	10 761 364	TACCACCGGTTCCATGTTTT	ATGGTGGTTCCAATGACGTT	AluⅠ	AGCT
SNP392	8	10 774 778	TGCATAATACTCATTCAACGCA	TTGATGCTTACTTTGGTGATGC	AluⅠ	AGCT
SNP485	8	10 839 486	TGGAAAAATGGCTTCAAATCT	TGCAAACGATTCGTCATTTC	AluⅠ	AGCT
SNP523	8	10 857 497	CATGGCGCTATGAGGTTTTT	TGCAAAATTTTCTTCCAATCG	MseⅠ	TTAA
SNP700	8	10 934 492	TGGAATGCAAACAAATGTGAA	GGACATCCGGTGTTGAACTT	AluⅠ	AGCT

群体 Generation	植株数 Plant number			分离比 Segregation ratio	卡方 Chi-test	P 值 P value
群体 Generation	总株数 Total	不脱蒂 None-abscission	脱蒂 Abscission	分离比 Segregation ratio	卡方 Chi-test	P 值 P value
P₁（M2-10）	30	30	0
P₂（ZT091）	30	0	30
F₁	30	30	0
F₂	189	110	79	9:7	0.173	0.678
BC₁P₁	153	153	0
BC₁P₂	146	115	31	3:1	0.481	0.817

群体 Generation	植株数 Plant number			分离比 Segregation ratio	卡方 Chi-test	P 值 P value
群体 Generation	总株数 Total	不脱蒂 None-abscission	脱蒂 Abscission	分离比 Segregation ratio	卡方 Chi-test	P 值 P value
P₁（M2-10）	30	30	0
P₂（ZT091）	30	0	30
F₁	30	30	0
F₂	189	110	79	9:7	0.173	0.678
BC₁P₁	153	153	0
BC₁P₂	146	115	31	3:1	0.481	0.817

分析方法 Analysis way	ED		Index		合集 Combination
分析方法 Analysis way	开始位点 Start	终止位点 Ending	开始位点 Start	终止位点 Ending	开始位点 Start	终止位点 Ending
SNP	10 685 509	11 693 628	10 686 821	11 693 628	10 686 821	11 693 628
InDel	10 428 865	11 108 980	10 459 389	11 042 074	10 459 389	11 042 074
合并区域 Combination region	10 686 821	11 042 074

Cytological Observation of Fruit Peduncle Abscission Zone and Preliminary Mapping of Mature Fruit Abscission AL3 gene in Melon

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