Screening and Culture Characteristics of Biocontrol Strains of Postharvest Anthracnose of Mango

doi:10.16420/j.issn.0513-353x.2020-0760

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (11): 2171-2184.doi: 10.16420/j.issn.0513-353x.2020-0760

• Original article • Previous Articles Next Articles

Screening and Culture Characteristics of Biocontrol Strains of Postharvest Anthracnose of Mango

ZHANG Xiaoyong, LI Shujiang, YAN Kai, WENG Guiying, ZHANG Yunxia, LONG Qiaofang, LIANG Wen, JIANG Qinna, YANG Youlian()

School of Biological Sciences and Technology,Liupanshui Normal University,Liupanshui,Guizhou 553004,China

Received:2021-04-22 Revised:2021-08-27 Published:2021-12-02
Contact: YANG Youlian E-mail:yangyoulianl@163.com

Abstract

Abstract:

Bacillus strains were isolated from Gentiana rhodantha stems with highly antifungal activity resist mango anthracnose was screened by confrontation culture with Colletotrachum gloeosporioides. The fungistatic effect of mycelial growth and conidia germination was measured by in vivo experiment. The control efficiency of antagonistic strain against anthracnose was tested by surface coating method using wounded mango fruit. The structure of the antifungal substances in fermentation broth of antagonistic bacteria was detected by high performance liquid chromatography-mass spectrometry（HPLC-MS）,and single factor experiment was used to study on the optimal growth and fermentation conditions,respectively. Based on morphological,physiological and 16S rDNA and gyrB sequences analysis,the strain SB023 which significant antifungal activity against mango anthracnose was identified as Bacillus velezensis. The mycelial growth and spore germination of pathogen were significantly inhibited by the fermentation broth of strain SB023. The control effect of SB023 to against anthracnose of mango reached 100% then coated with 0.1% Tween-80,2% sodium alginate and 10% fermentation broth. The main antifungal substances were Surfactin C,Bacillomycin D,Bacillomycin L and Iturin A in broth. The fermentation study showed that the optimal culture medium for KMB with yeast extract,maltose,magnesium sulfate,initial pH 7.0 and incubated at 28-30 ℃.

Key words: mango, anthracnose, Bacillus velezensis, antagonistic bacteria, antifungal substance, fermentative conditions

CLC Number:

S667.7

ZHANG Xiaoyong, LI Shujiang, YAN Kai, WENG Guiying, ZHANG Yunxia, LONG Qiaofang, LIANG Wen, JIANG Qinna, YANG Youlian. Screening and Culture Characteristics of Biocontrol Strains of Postharvest Anthracnose of Mango[J]. Acta Horticulturae Sinica, 2021, 48(11): 2171-2184.

Figures/Tables 10

References 41

[1]	Bayisa R A. 2020. Enhancing resistance of Sesamum indicum against Alternaria sesami through Bacillus velezensis AR1. Pest Management Science,doi: 10.1002/ps.5890. doi: 10.1002/ps.5890
[2]	Buchanan R E, Gibbons N E. 1994. Bergey’s manual of systematic bacteriology. 9th ed. Baltimore:Williams and Wilkins Company,
[3]	Damasceno Caroline, Duarte E, Santos L, Oliveira T, Jesus F, Oliveira L, Góes-Neto A, Soares A. 2019. Postharvest biocontrol of anthracnose in bananas by endophytic and soil rhizosphere bacteria associated with sisal(Agave sisalana)in Brazil. Biological Control, 137:104016. doi: 10.1016/j.biocontrol.2019.104016 URL
[4]	Dong Xiuzhu, Cai Miaoying. 2001. Manual for systematic identification of common bacteria. Beijing: Science Press. (in Chinese)
	东秀珠, 蔡妙英. 2001. 常见细菌系统鉴定手册. 北京: 科学出版社.
[5]	Dou Yanan, Niu Shiquan, Dou Jiantao, Zhao Dan, Zheng Doudou, Zhou Xuan, Wang Yan, Zhu Xuetai, Kong Weibao. 2018. Screening,identification and preliminary study on biocontrol effect of antagonistic Bacillus against the pathogen of Valsa mali for apple tree. Microbiology China, 45 (12):2684-2694.
	豆雅楠, 牛世全, 豆建涛, 赵丹, 郑豆豆, 周璇, 王彦, 朱学泰, 孔维宝. 2018. 芽孢杆菌拮抗苹果树腐烂病菌的筛选、鉴定及抑菌活性初探. 微生物学通报, 45 (12):2684-2694.
[6]	Duca M T L, Satomib M, Agatac N, Venkateswarana K. 2004. GyrB as a phylogenetic discriminator for members of the Bacillus anthracis-cereus-thuringiensis group. Journal of Microbiological Methods, 56 (3):383-394. doi: 10.1016/j.mimet.2003.11.004 URL
[7]	Farungsang U, Sinlapasunthorn S, Rattanakreetakul C, Phavaphutanon L, Farungsang N. 2014. Bacillus megaterium isolate 3103:antagonistic spectrum on Colletotrichum gloeosporioides diversity and impact of field application on postharvest incidence of mango fruit anthracnose. International Society for Horticultural Science, 973:81-88.
[8]	Gong Deqiang, Gao Zhaoyin, Li Min, Hu Meijiao, Li Chunxia, Chen Qianfu, Huang Huili, Yang Jinying, Huang Taiming, Zhu Shijiang. 2019. Effects of pre-harvest salicylic acid spraying on postharvest quality and disease resistance of mango fruit. Shandong Agricultural Sciences, 51 (8):91-96.
	弓德强, 高兆银, 李敏, 胡美姣, 李春霞, 陈千付, 黄慧俐, 杨谨瑛, 黄台明, 朱世江. 2019. 采前水杨酸处理对芒果采后品质及抗病性的影响. 山东农业科学, 51 (8):91-96.
[9]	Gu Q, Yang Y, Yuan Q, Shi G, Wu L, Lou Z, Huo R, Wu H, Borriss R, Gao X. 2017. Bacillomycin D produced by Bacillus amyloliquefaciens is involved in the antagonistic interaction with the plant pathogenic fungus Fusarium graminearum. Applied and Environmental Microbiology, 83 (19):AEM.01075-17.
[10]	Harun-Or-Rashid M, Khan A, Hossain M T, Chung Y R. 2017. Induction of systemic resistance against aphids by endophytic Bacillus velezensis YC 7010 via expressing PHYTOALEXIND EFICIENT4 in Arabidopsis. Frontiers in Plant Science, 8 (211):1-12.
[11]	He Meiyu. 2002. Modern organic and biological mass spectrometry. Beijing: Peking University Press. (in Chinese)
	何美玉. 2002. 现代有机与生物质谱. 北京: 北京大学出版社.
[12]	Jang S J, Kim Y J, Park J M, Park Y S. 2011. Analysis of microflora in Gochujang,Korean traditional fermented food. Food Science & Biotechnology, 20 (5):1435-1440.
[13]	Jeffrey D, Wisotzkey P, Jurtshuk J R, George E, Fox G D, Karl P. 1992. Comparative sequence analyses on the 16s rDNA(rDNA)of Bacillus acidocaldarius,Bacillus acidoterrestris,and Bacillus cycloheptanicus and proposal for creation of a new genus,Alicyclobacillus gen. nov. International Journal of Systematic and Evolutionary Microbiology, 42 (2):263-269.
[14]	Ji Z H, He H W, ZhouH J L, Han F, Tong Y H, Ye Z W, Xu J Y. 2015. The biocontrol effects of the bacillus licheniformis W 10 strain and its antifungal protein against brown rot in peach. Horticultural Plant Journal, 1 (3):131-138.
[15]	Ji Zuoliang, Dai Hongfen, Zhang Zhaoqi. 1998. Changes of ascorbic acid and glutatione contents during chilling injury of mango fruit. Acta Horticulturae Sinica, 25 (4):3-5. (in Chinese)
	季作梁, 戴宏芬, 张昭其. 1998. 杧果果实冷害过程中谷胱甘肽和抗坏血酸含量的变化. 园艺学报, 25 (4):3-5.
[16]	Jin P F, Wang H N, Tan Z, Xuan Z, Dahar G Y, Li Q X, Miao W G, Liu W B. 2020. Antifungal mechanism of bacillomycin D from Bacillus velezensis HN-2 against Colletotrichum gloeosporioides penz. Pesticide Biochemistry and Physiology, 163:102-107. doi: 10.1016/j.pestbp.2019.11.004 URL
[17]	Koomen I, Jeffries P. 2007. Effects of antagonistic organisms on the postharvest development of Colletotrichum gloeosporioides on mango. Plant Pathology, 42:230-237. doi: 10.1111/ppa.1993.42.issue-2 URL
[18]	Li Chunling. 2012. Screening and bio-control potential determination of some antagonistic bacteria isolates for controlling anthracnose diseases of mango[M. D. Dissertation]. Tai’an: Shandong Agricultural University. (in Chinese)
	李春玲. 2012. 芒果炭疽病拮抗细菌筛选及生防潜能测定[硕士论文]. 泰安: 山东农业大学.
[19]	Li Jiawei, Xu Lanyi, Wang Dongshuang, Lü Lijie, Chen Xiaomeng, Zhang Dongdong. 2019. Screening and identification of Bacillus antagonist against anthracnose of jujube and analysis of its antifungal substance. Acta Horticulturae Sinica, 46 (12):2406-2414. (in Chinese)
	李嘉维, 徐兰依, 王冬霜, 吕丽洁, 陈晓萌, 张冬冬. 2019. 枣炭疽病菌拮抗芽孢杆菌的筛选鉴定及其抑菌物质分析. 园艺学报, 46 (12):2406-2414.
[20]	Liu G, Kong Y, Fan Y, Geng C, Peng D, Sun M. 2017. Whole-genome sequencing of Bacillus velezensis lS69,a strain with a broad inhibitory spectrum against pathogenic bacteria. Journal of Biotechnology, 249:20. doi: 10.1016/j.jbiotec.2017.03.018 URL
[21]	Lu Deling, Wei Shuyan, Wei Hongfei, Cao Hongyu, Cui Shasha, Zhao Man, Huang Yanchang. 2017. Joint toxicity of Difenoconazole- Azoxystrobin mixture against Zizyphus jujube anthracnose and effect on field trial. Agrochemicals, 56 (1):61-64. (in Chinese)
	陆德玲, 魏书艳, 魏洪飞, 曹洪玉, 崔沙沙, 赵曼, 黄延昌. 2017. 苯醚甲环唑、嘧菌酯对冬枣炭疽病的联合毒力与田间防效. 农药, 56 (1):61-64.
[22]	Lu Xiaocheng. 2006. Studies on antifungal active substance of a Bacillus subtilis strain[M. D. Dissertation]. Hangzhou: Zhejiang University. (in Chinese)
	鲁小城. 2006. 一株枯草芽孢杆菌抗植物病原真菌活性物质的研究[硕士论文]. 杭州: 浙江大学.
[23]	Lü Qianqian, Zhao Xinggang, Wang Dongdong, Mao Xia, Zuo Cunwu, Yang Jiangshan. 2020. Identification of Bacillus amyloliquefaciens BaA-007 and its inhibitory effect on apple canker. Acta Horticulturae Sinica, 47 (10):1895-1904. (in Chinese)
	吕前前, 赵兴刚, 王东东, 冒霞, 左存武, 杨江山. 2020. 解淀粉芽孢杆菌BaA-007鉴定及其对苹果腐烂病的抑制作用. 园艺学报, 47 (10):1895-1904.
[24]	Mu Xiangyi, Zhang Xuan, Hu Youzhen, Guan Bo, Chen Guogang. 2020. Screening and identification of an antagonistic bacterium against blackhead disease of Korla fragrant pear. Microbiology China, 47 (6):1795-1806.
	穆香轶, 张璇, 胡有贞, 关波, 陈国刚. 2020. 库尔勒香梨黑头病拮抗菌的筛选和鉴定. 微生物学通报, 47 (6):1795-1806.
[25]	Nam M H, Kim H S, Lee H D, Whang K S, Kim H G. 2014. Biological control of anthracnose crown rot in strawberry using Bacillus velezensis NSB-1. Acta Horticulturae, 1049:685-688.
[26]	Ramarathnam R, Bo S, Chen Y, Fernando W G D, Xuewen G, Kievit T D. 2007. Molecular and biochemical detection of Fengycin and Bacillomycin D-producing Bacillus spp. antagonistic to fungal pathogens of canola and wheat. Canadian Journal of Microbiology, 53 (7),901-911. pmid: 17898845
[27]	Ren Yanfang, Xue Yuhao, Tian Dan, He Junyu, Zhang Liming, Wu Qing, Liu Shu, 2021. Synergistic effect of salicylic acid and nitric oxide treatment on quality and antioxidant activity in postharvest mango. Food Science, 42 (9):151-159. (in Chinese) doi: 10.1111/jfds.1977.42.issue-1 URL
	任艳芳, 薛宇豪, 田丹, 何俊瑜, 张黎明, 吴情, 刘树. 2021. 水杨酸和硝普钠协同处理对芒果贮藏品质及抗氧化活性的影响. 食品科学, 42 (9):151-159
[28]	Romero D, de Vicente A, Rakotoaly R H, Dufour S E, Veening J W, Arrebola E, Cazorla F M, Kuipers O P, Paquot M, Perez-Garcia A. 2007. The Iturin and Fengycin families of lipopeptides are key factors in antagonism of Bacillus subtilis toward Podosphaera fusca. Molecular Plant Microbe Interact, 20:430-440. doi: 10.1094/MPMI-20-4-0430 URL
[29]	Ruiz-García C, Bejar V, Martínez-Checa F, Llamas I, Quesada E. 2005. Bacillus velezensis sp. nov. a surfactant-producing bacterium isolated from the river Velez in Malaga,southern Spain. International Journal of Systematic and Evolutionary Microbiology, 55 (1):191-195. doi: 10.1099/ijs.0.63310-0 URL
[30]	Shen Shunshan, Zhang Tao, Wang Juan, Liu Dongping, Zhang Shanshan, Sun Zhiqiang, Piao Fengzhi. 2019. Antifungal activity of Paenibacillus polymyxa HK18- 8 against pepper anthracnose and its colonization ability. Acta Horticulturae Sinica, 46 (3):499-507. (in Chinese)
	申顺善, 张涛, 王娟, 刘东平, 张珊珊, 孙治强, 朴凤植. 2019. 多粘类芽孢杆菌HK18-8对辣椒炭疽病菌的抑制作用及其定殖能力. 园艺学报, 46 (3):499-507.
[31]	Sun P P, Cui J C, Jia X H, Wang W H. 2017. Isolation and characterization of bacillus amyloliquefaciens L-1 for biocontrol of pear ring rot. Horticultural Plant Journal, 3 (5):183-189. doi: 10.1016/j.hpj.2017.10.004 URL
[32]	Trinh T H T, Wang S L, Nguyen V B, Tran M D, Nguyen A D. 2019. A potent antifungal rhizobacteria Bacillus velezensis RB.DS29 isolated from black pepper(Piper nigrum L.). Research on Chemical Intermediates 45 (11):5309-5323. doi: 10.1007/s11164-019-03971-5 URL
[33]	Wang Yu, Tan Zheng, Wei Dandan, Miao Weiguo, Liu Wenbo, Jin Pengfei. 2020. Identification of Bacillus HN-2 and analysis of its antifungal properties. Chinese Journal of Biological Control, 36 (2):220-230. (in Chinese)
	王雨, 谭峥, 韦丹丹, 缪卫国, 刘文波, 靳鹏飞. 2020. 贝莱斯芽胞杆菌HN-2的鉴定及对杧果炭疽菌的抑菌活性研究. 中国生物防治学报, 36 (2):220-230.
[34]	Waseem R, Yang X M, Wu H S, Wang Y, Xu Y C, Shen Q R. 2009. Isolation and characterization of fusaricidin-type compound-producing strain of Paenibacillus polymyxa SQR-21 active against Fusarium oxysporum f. sp. nevium. European Journal of Plant Pathology, 125 (3):471-483. doi: 10.1007/s10658-009-9496-1 URL
[35]	Xiang N, Lawrence K S, Kloepper J W, Donald P A, McInroy J A. 2017. Biological control of Meloidogyne incognita by spore-forming plant growth-promoting rhizobacteria on cotton. Plant Disease, 101:774-784. doi: 10.1094/PDIS-09-16-1369-RE pmid: 30678579
[36]	Yang Bo. 2013. Survey and identification of fungal disease for mango fruits in Hainan[M. D. Dissertation]. Haikou: Hainan University. (in Chinese)
	杨波. 2013. 海南芒果采后真菌病害调查及病原鉴定[硕士论文]. 海口: 海南大学.
[37]	Yang Youlian, Liu Yongxiang, Liu Zuoyi. 2014. Colletotrichum species responsible for Anthracnose disease of postharvest fruits. Southwest Agricultural Journal, 27 (3):1114-1123. (in Chinese)
	杨友联, 刘永翔, 刘作易. 2014. 水果采后炭疽病病原鉴定. 西南农业学报, 27 (3):1114-1123.
[38]	Yu Feijin, Chen Weiliang. 2006. Effects of culture conditions on the production of antagonistic substance produced by Bacillus cereu 357. Journal of Zhejiang University(Agriculture and Life Sciences),(4):396-400. (in Chinese)
	于飞进, 陈卫良. 2006. 培养条件对Bacillus cereus 357生长及产生拮抗物质的影响. 浙江大学学报(农业与生命科学版),(4):396-400.
[39]	Yuan Xue, Hou Xu, Hu Xiao, Chang Haotian, Yang Rui, Liu Yueping. 2018. Screening and identification of antagonistic bacteria and its control effect against the peach brown rot. Journal of Beijing University of Agriculture, 33 (4):7-13. (in Chinese)
	袁雪, 侯旭, 胡晓, 常昊天, 杨瑞, 刘悦萍. 2018. 桃褐腐病拮抗细菌的筛选、鉴定及生防作用. 北京农学院学报, 33 (4):7-13.
[40]	Zhang Y, Gao X, Wang S, Zhu C, Li R, Shen Q. 2018. Application of Bacillus velezensis NJZU-Z9 enhanced plant growth associated with efficient rhizospheric colonization monitored by qPCR with primers designed from the whole genome sequence. Current Microbiology, 75:1574-1583. doi: 10.1007/s00284-018-1563-4 pmid: 30194479
[41]	Zhou Deqing. 2002. Microbiology experiment teaching. 2nd ed. Beijing: Higher Education Press. (in Chinese)
	周德庆. 2002. 微生物学实验教程. 2版北京: 高等教育出版社.

发酵液添加量/% Contents of fermentation broth	菌落直径/mm Colony diam	菌丝生长抑制率/% Mycelial growth rate	孢子萌发率/% Spore germination	孢子萌发抑制率/% Inhibition rate to spore germination	孢子芽管生长率/ （μm · d^-1） Growth rate of germ tube
0（对照Control）	90.00 ± 0.00 a	—	97.23 ± 3.24 a	—	257.83 ± 18.04 a
2.5	55.10 ± 1.41 b	38.89 ± 1.57 e	78.84 ± 4.33 b	18.91 ± 2.20 c	241.33 ± 6.03 a
5.0	41.30 ± 1.41 c	54.44 ± 1.57 d	34.47 ± 5.56 c	64.55 ± 5.45 b	183.33 ± 23.15 b
10	24.50 ± 0.71 d	72.78 ± 0.79 c	10.23 ± 2.32 d	100.00 ± 0.00 a	0 c
15	11.50 ± 0.71 e	87.22 ± 0.79 b	0 e	100.00 ± 0.00 a	0 c
20	7.10 ± 0.14 f	92.11 ± 0.16 a	0 e	100.00 ± 0.00 a	0 c

发酵液添加量/% Contents of fermentation broth	菌落直径/mm Colony diam	菌丝生长抑制率/% Mycelial growth rate	孢子萌发率/% Spore germination	孢子萌发抑制率/% Inhibition rate to spore germination	孢子芽管生长率/ （μm · d^-1） Growth rate of germ tube
0（对照Control）	90.00 ± 0.00 a	—	97.23 ± 3.24 a	—	257.83 ± 18.04 a
2.5	55.10 ± 1.41 b	38.89 ± 1.57 e	78.84 ± 4.33 b	18.91 ± 2.20 c	241.33 ± 6.03 a
5.0	41.30 ± 1.41 c	54.44 ± 1.57 d	34.47 ± 5.56 c	64.55 ± 5.45 b	183.33 ± 23.15 b
10	24.50 ± 0.71 d	72.78 ± 0.79 c	10.23 ± 2.32 d	100.00 ± 0.00 a	0 c
15	11.50 ± 0.71 e	87.22 ± 0.79 b	0 e	100.00 ± 0.00 a	0 c
20	7.10 ± 0.14 f	92.11 ± 0.16 a	0 e	100.00 ± 0.00 a	0 c

处理 Treatment	病斑面积/ mm²Lessons area	抑制率/% Inhibition rate	病情指数 Disease index	防治效果/% Control efficiency
涂膜剂 + 菌悬液 + 接病菌 Coating agents + suspension + inoculation	0 c	100.00 ± 0.00 a	0 b	100.00 ± 0.00 a
涂膜剂 + 发酵液 + 接病菌 Coating agents + fermentation broth + inoculation	0 c	100.00 ± 0.00 a	0 b	100.00 ± 0.00 a
涂膜剂 + 菌悬液Coating agents + suspension	0 c	—	0 b	—
涂膜剂 + 接病菌Coating agents + inoculation	189.50 ± 45.05 a	5.50 ± 1.45 c	75.35 ± 3.25 a	4.17 ± 1.23 c
接病菌 + 涂膜剂 + 发酵液 Inoculation + coating agents + fermentation broth	135.25 ± 21.21 b	32.37 ± 7.24 b	71.33 ± 4.54 a	9.28 ± 1.67 b
接病菌（对照）Inoculation（Control）	201.55 ± 53.85 a	—	78.63 ± 6.25 a	—

处理 Treatment	病斑面积/ mm²Lessons area	抑制率/% Inhibition rate	病情指数 Disease index	防治效果/% Control efficiency
涂膜剂 + 菌悬液 + 接病菌 Coating agents + suspension + inoculation	0 c	100.00 ± 0.00 a	0 b	100.00 ± 0.00 a
涂膜剂 + 发酵液 + 接病菌 Coating agents + fermentation broth + inoculation	0 c	100.00 ± 0.00 a	0 b	100.00 ± 0.00 a
涂膜剂 + 菌悬液Coating agents + suspension	0 c	—	0 b	—
涂膜剂 + 接病菌Coating agents + inoculation	189.50 ± 45.05 a	5.50 ± 1.45 c	75.35 ± 3.25 a	4.17 ± 1.23 c
接病菌 + 涂膜剂 + 发酵液 Inoculation + coating agents + fermentation broth	135.25 ± 21.21 b	32.37 ± 7.24 b	71.33 ± 4.54 a	9.28 ± 1.67 b
接病菌（对照）Inoculation（Control）	201.55 ± 53.85 a	—	78.63 ± 6.25 a	—

Screening and Culture Characteristics of Biocontrol Strains of Postharvest Anthracnose of Mango

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 10

References 41

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHAO Zhichang, HUANG Jianfeng, LUO Ruixiong, GAO Aiping, CHEN Yeyuan, WANG Guangying, CHEN Jingfeng, GONG Deyong, and DANG Zhiguo, . A New Mango Cultivar‘Hongyu’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 55-56.
[2]	ZHAO Zhichang, HUANG Jianfeng, LUO Ruixiong, GAO Aiping, CHEN Yeyuan, WANG Guangying, CHEN Jingfeng, GONG Deyong, and DANG Zhiguo, . A New Mango Cultivar‘Fenghuang’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 57-58.
[3]	GONG Changyi, LIU Jiaojiao, DENG Qiang, ZHANG Lixin. Identification and Pathogenicity of Colletotrichum Species Causing Anthracnose on Camellia sinensis [J]. Acta Horticulturae Sinica, 2022, 49(5): 1092-1101.
[4]	WU Hongxia, LI Xing, XU Wentian, ZHENG Bin, MA Xiaowei, SU Muqing, LIANG Qingzhi, YAO Quansheng, WANG Songbiao. Genetic Analysis of Fruit Traits in F₁ Progenies of ‘Chinhuang’×‘Renong 1’Mango [J]. Acta Horticulturae Sinica, 2022, 49(2): 416-426.
[5]	YU Lin, SHE Xiaoman, TANG Yafei, LAN Guobing, LI Zhenggang, HE Zifu. Identification of Pathogen Causing Allium ascalonicum Anthracnose in Guangdong [J]. Acta Horticulturae Sinica, 2022, 49(1): 129-140.
[6]	YAN Zhubing, WANG Mei, MING Changjun, JIANG Yuanmao, SHEN Xiang, CHEN Xuesen, YIN Chengmiao, MAO Zhiquan. Screening and Identification of Bacillus velezensis XC1 and Its Influence on Apple Replant Disease [J]. Acta Horticulturae Sinica, 2021, 48(3): 409-420.
[7]	LI Xinyu1,2，LI Lei1，CHEN Lida1，SHI Yanxia1，CHAI Ali1，XIE Xuewen1,，and LI Baoju1,. Screening and Identification of Antagonistic Bacteria Against Gray Leaf Spot of Tomato Caused by Stemphylium solani [J]. ACTA HORTICULTURAE SINICA, 2020, 47(4): 741-748.
[8]	WANG Hong，YUE Kun，YANG Chengkun，GUO Yujian，WANG Peixuan，and ZHOU Kaibing. Physiological Reasons for Inhibiting Photosynthesis of Mango Leaves by Enhanced UV-B Radiation [J]. ACTA HORTICULTURAE SINICA, 2020, 47(2): 242-252.
[9]	WANG Tongtong，GAO Fen，LIU Dan，and LI Peili*. Primary Identification of the Pathogen Causing Anthracnose of Nandina domestica in Chengdu，Sichuan [J]. ACTA HORTICULTURAE SINICA, 2019, 46(8): 1576-1584.
[10]	ZHOU Kaibing*，LI Shijun，YUAN Mengling，and YUE Kun. Injuries and the Responses on Antioxidation of Adult Mango Trees Under the Treatments of Enhanced UV-B Radiation [J]. ACTA HORTICULTURAE SINICA, 2019, 46(7): 1279-1289.
[11]	LI Jiawei，XU Lanyi，WANG Dongshuang，Lü Lijie，CHEN Xiaomeng，and ZHANG Dongdong*. Screening and Identification of Bacillus Antagonist Against Anthracnose of Jujube and Analysis of Its Antifungal Substances [J]. ACTA HORTICULTURAE SINICA, 2019, 46(12): 2406-2414.
[12]	MA Xiaowei，MA Yongli，WU Hongxia，ZHOU Yigang，SU Muqing，and WANG Songbiao*. Assessment of Mineral Elements Contents at the Mango Germplasm Level Based on Factor Analysis and Cluster Analysis [J]. ACTA HORTICULTURAE SINICA, 2018, 45(7): 1371-1381.
[13]	ZHOU Dengbo1，JING Tao2,，QI Dengfeng1，FENG Renjun1，DUAN Yajie3，CHEN Yufeng1，WANG Fei1，ZHANG Xiyan1，and XIE Jianghui1,*. Isolation and Identification of Streptomyces lunalinharesii and its Control Effect on the Banana Fusarium Wilt Disease [J]. ACTA HORTICULTURAE SINICA, 2017, 44(4): 664-674.
[14]	HUANG Guo-di*，LI Ri-wang，GUO Li-mei，MO Yong-long，ZHAO Ying，ZHANG Yu，and CHEN Yong-sen. A New Mango Cultivar‘Guiremang 4’ [J]. ACTA HORTICULTURAE SINICA, 2016, 43(S2): 2719-2720.
[15]	MA Xiao-wei，YANG Ying-di，WU Hong-xia，ZHOU Yi-gang，and WANG Song-biao* . Diversity Analysis of Volatile in Mature Fruits of Mango Germplasm [J]. ACTA HORTICULTURAE SINICA, 2016, 43(7): 1267-1274.