Screening and Validation of Interacting Proteins of the Grape Ovule Development Transcription Factor VvDDF2

doi:10.16420/j.issn.0513-353x.2024-0889

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (11): 2846-2860.doi: 10.16420/j.issn.0513-353x.2024-0889

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Screening and Validation of Interacting Proteins of the Grape Ovule Development Transcription Factor VvDDF2

LIU Miaomiao, YAO Jin, ZOU Fuyan, CHU Yanyan, and WANG Xiping^*()

State Key Laboratory for Crop Stress Resistance and High-Efficiency Production， Key Laboratory of Northwest Horticultural Crop Biology and Germplasm Creation，Ministry of Agriculture and Rural Affairs，College of Horticulture，Northwest A & F University，Yangling， Shaanxi 712100， China

Received:2025-03-22 Revised:2025-07-02 Online:2025-11-26 Published:2025-11-26
Contact: and WANG Xiping

Abstract

Abstract:

In order to investigate the regulatory mechanism of the grape transcription factor VvDDF2 in ovule development and seedless traits formation，the yeast two-hybrid bait vector pGBKT7-VvDDF2 was constructed. It was verified that VvDDF2 had transcriptional self-activation activity and the active region was located at the C terminal. Using yeast two-hybrid system（Y2H），14 candidate interacting proteins of VvDDF2 were screened from the yeast cDNA yeast library related to the development of grape ovule，including lipase，amine oxidase，exocyst complex component，large ribosomal subunit protein and other enzymes and proteins，which are mainly involved in cell division，growth，and morphogenesis，embryonic development，plant hormone signal transduction and fertility regulation，as well as biosynthesis and metabolic reactions of various substances，and play an important biological function in the process of plant seed development. The interaction between VvDDF2 and VvEXO70A1 was further verified by yeast two-hybrid system（Y2H），bimolecular fluorescence complementation（BiFC）and luciferase reporter system（Split-Luc）. RT-qPCR analysis showed that the expression level of VvEXO70A1 during the development of grape ovule were significantly different between seeded and seedless grapes. In summary，VvDDF2 and VvEXO70A1 may form a complex through protein interaction to synergistically regulate the development process of grape ovule.

Key words: grape, seedless mechanism, ovule development, VvDDF2, protein interaction, VvEXO70A1

LIU Miaomiao, YAO Jin, ZOU Fuyan, CHU Yanyan, and WANG Xiping. Screening and Validation of Interacting Proteins of the Grape Ovule Development Transcription Factor VvDDF2[J]. Acta Horticulturae Sinica, 2025, 52(11): 2846-2860.

Figures/Tables 8

References 40

[1]	Ahmad B, Zhang S L, Yao J, Chai S Y, Yadav V, Athar H R, Rahman M U, Wang L, Wang X P. 2022. Ectopic expression of VvFUS,B3 domain transcription factor,in tomato influences seed development via affecting endoreduplication and hormones. Horticultural Plant Journal, 8 (3):351-360.
[2]	Chen Tianchi, Zhang Qiao, Wang Jinyang, Zhang Min, Xiao Xuteng, Dong Yiling, Shen Leyi, Li Jiacheng, Jiang Yuqi, Wu Yueyan. 2023. Cloning and bioinformatics analysis of HDR gene from‘Yinhong’grape. Molecular Plant Breeding, 21 (2):409-419. (in Chinese)
	陈天池, 张巧, 王锦阳, 张敏, 肖旭腾, 董昳伶, 沈乐意, 李佳程, 姜雨琦, 吴月燕. 2023. ‘鄞红’葡萄HDR基因的克隆与生物信息学分析. 分子植物育种, 21 (2):409-419.
[3]	Clauss K, von Roepenack-Lahaye E, Böttcher C, Roth M R, Welti R, Erban A, Kopka J, Scheel D, Milkowski C, Strack D. 2011. Overexpression of sinapine esterase BnSCE3 in oilseed rape seeds triggers global changes in seed metabolism. Plant Physiology, 155 (3):1127-1145.
[4]	Cui Mengjie, Wang Chen, Zhang Wenying, Tang Wei, Zhu Xudong, Li Xiaopeng, Fang Jinggui. 2017. Research progress of seedless grape. Plant Physiology Journal, 53 (3):317-330. (in Chinese)
	崔梦杰, 王晨, 张文颖, 汤崴, 朱旭东, 李晓鹏, 房经贵. 2017. 无核葡萄研究进展. 植物生理学报, 53 (3):317-330.
[5]	Ding Xianlong. 2017. Identification and functional study of differential miRNA in cytoplasmic-nuclear male sterile line NJCMS1A and its maintainer,restorer of soybean(Glycine Max(L.)Merr.)[Ph. D. Dissertation]. Nanjing: Nanjing Agricultural University. (in Chinese)
	丁先龙. 2017. 大豆质核互作雄性不育系NJCMS1A与其保持系、恢复系的差异miRNA鉴定及功能研究[博士论文]. 南京: 南京农业大学.
[6]	Dong J, Kim S T, Lord E M. 2005. Plantacyanin plays a role in reproduction in Arabidopsis. Plant Physiology, 138 (2):778-789.
[7]	Fang X, Lin Y L, Chen C, Tariq P, Wang X C, Luo H F, Fang J G, Shangguan L F. 2023. Whole genome identification of CBF gene families and expression analysis in Vitis vinifera L. Czech Journal of Genetics and Plant Breeding, 59 (3):119-132.
[8]	Fortes A M, Agudelo-Romero P, Pimentel D, Alkan N. 2019. Transcriptional modulation of polyamine metabolism in fruit species under abiotic and biotic stress. Frontiers in Plant Science,10:816.
[9]	Gong P J, Zhang J H, Li H X, Yang C X, Zhang C J, Zhang X H, Khurram Z, Zhang Y Y, Wang T T, Fei Z J, Ye Z B. 2010. Transcriptional profiles of drought-responsive genes in modulating transcription signal transduction,and biochemical pathways in tomato. Journal of Experimental Botany, 61 (13):3563-3575.
[10]	Hala M, Cole R, Synek L, Drdova E, Pecenkova T, Nordheim A, Lamkemeyer T, Madlung J, Hochholdinger F, Fowler J E. 2008. An exocyst complex functions in plant cell growth in Arabidopsis and tobacco. Plant Cell, 20 (5):1330-1345.
[11]	Ji Wei, Guo Rongrong, Wang Jingbo, Jiao Xiaobo, Yan Zhao, Chang Qinxiang, Dong Zhigang, Wang Yuejin. 2019. Grey correlation analysis of physiological and biochemical factors in embryo abortion of seedless grape. Acta Horticulturae Sinica, 46 (8):1473-1485. (in Chinese)
	纪薇, 郭荣荣, 王静波, 焦晓博, 闫钊, 昌秦湘, 董志刚, 王跃进. 2019. 无核葡萄胚败育生理生化因子灰色关联分析. 园艺学报, 46 (8):1473-1485.
[12]	Julian J, Coego A, Lozano-Juste J, Lechner E, Wu Q, Zhang X, Merilo E, Belda-Palazon B, Park S Y, Cutler S R, An C C, Genschik P, Rodriguez P L. 2019. The MATH-BTB BPM3 and BPM5 subunits of Cullin3-RING E3 ubiquitin ligases target PP2CA and other clade A PP2Cs for degradation. Proceedings of the National Academy of Sciences of the United States of America, 116 (31):15725-15734.
[13]	Khan F. 2020. The functional analysis of BnaRPLS34 in the self-incompatibility response of Brassica napus. L[M. D. Dissertation]. Wuhan: Huazhong Agricultural University. (in Chinese)
	Khan F. 2020. 甘蓝型油菜核糖体大亚基BnaRPLS34基因调控自交不亲和反应的功能研究[硕士论文]. 武汉: 华中农业大学.
[14]	Kulich I, Cole R, Drdová E, Cvrčková F, Soukup A, Fowler J, Žárský V. 2010. Arabidopsis exocyst subunits SEC8 and EXO70A1 and exocyst interactor ROH1 are involved in the localized deposition of seed coat pectin. The New Phytologist, 188 (2):615-625.
[15]	Li S P, van Os G M, Ren S C, Yu D L, Ketelaar T, Emons A M, Liu C M. 2010. Expression and functional analyses of EXO70 genes in Arabidopsis implicate their roles in regulating cell type-specific exocytosis. Plant Physiology, 154 (4):1819-1830.
[16]	Li Z Q, Jiao Y T, Zhang C, Dou M R, Weng K, Wang Y J, Xu Y. 2021. VvHDZ28 positively regulate salicylic acid biosynthesis during seed abortion in Thompson Seedless. Plant Biotechnology Journal, 19 (9):1824-1838.
[17]	Li Shasha, Wang Yuejin. 2019. Advances in seedless gene researches and seedless breeding in grapevine. Acta Horticulturae Sinica, 46 (9):1711-1726. (in Chinese)
	李莎莎, 王跃进. 2019. 葡萄无核基因及无核育种研究进展. 园艺学报, 46 (9):1711-1726.
[18]	Liu Miaomiao, Yao Jin, Bao Min, Chu Yanyan, Wang Xiping. 2024. Research progress on the mechanism of GAs-induced seedlessness in grape. Acta Horticulturae Sinica, 51 (7):1610-1622. (in Chinese)
	刘苗苗, 姚锦, 包敏, 楚言言, 王西平. 2024. 赤霉素诱导葡萄无核形成机理研究进展. 园艺学报, 51 (7):1610-1622.
[19]	Lyu X G, Li Y H, Li Y F, Li D L, Han C, Hong H L, Tian Y, Han L D, Liu B, Qiu L J. 2023. The domestication-associated L1 gene encodes a eucomic acid synthase pleiotropically modulating pod pigmentation and shattering in soybean. Molecular Plant, 16 (7):1178-1191.
[20]	Magome H, Yamaguchi S, Hanada A, Kamiya Y, Oda K. 2004. Dwarf and delayed-flowering 1,a novel Arabidopsis,mutant deficient in gibberellin biosynthesis because of overexpression of a putative AP2 transcription factor. The Plant Journal, 37 (5):720-729.
[21]	Malabarba J, Buffon V, Mariath J E A, Gaeta M L, Dornelas M C, Margis-Pinheiro M, Pasquali G, Revers L F. 2017. The MADS-box gene Agamous-like 11 is essential for seed morphogenesis in grapevine. Journal of Experimental Botany, 68 (7):1493-1506.
[22]	Mejía N, Soto B, Guerrero M, Casanueva X, Houel C, Miccono Mde L, Ramos R, Le Cunff L, Boursiquot J M, Hinrichsen P, Adam-Blondon A F. 2011. Molecular,genetic and transcriptional evidence for a role of VvAGL11 in stenospermocarpic seedlessness in grapevine. BMC Plant Biology,11:57.
[23]	Mizoi J, Shinozaki K, Yamaguchi-Shinozaki K. 2012. AP2/ERF family transcription factors in plant abiotic stress responses. Biochimica et Biophysica Acta. Gene Regulatory Mechanisms, 1819 (2):86-96.
[24]	Rezaei M K, Shobbar Z S, Shahbazi M, Abedini R, Zare S. 2013. Glutathione S-transferase(GST)family in barley:identification of members,enzyme activity,and gene expression pattern. Journal of Plant Physiology, 170 (14):1277-1284.
[25]	Royo C, Torres-Pérez R, Mauri N, Diestro N, Cabezas J A, Marchal C, Lacombe T, Ibáñez J, Tornel M, Carreño J, Martínez-Zapater J M, Carbonell-Bejerano P. 2018. The major origin of seedless grapes is associated with a missense mutation in the MADS-box gene VviAGL11. Plant Physiology, 177 (3):1234-1253.
[26]	Sakuma Y, Liu Q, Dubouzet J G, Abe H, Shinozak K, Yamaguchi-Shinozaki K. 2002. DNA-binding specificity of the ERF/AP 2 domain of Arabidopsis DREBs,transcription factors involved in dehydration- and cold-inducible gene expression. Biochemical and Biophysical Research Communications, 290 (3):998-1009.
[27]	Samuel M A, Chong Y T, Haasen K E, Aldea-Brydges M G, Stone S L, Goring D R. 2009. Cellular pathways regulating responses to compatible and self-incompatible pollen in Brassica and Arabidopsis stigmas intersect at EXO70A1,a putative component of the exocyst complex. The Plant Cell, 21 (9):2655-2671.
[28]	Synek L, Schlager N, Eliás M, Quentin M, Hauser Mt, Zárský V. 2006. AtEXO70A1,a member of a family of putative exocyst subunits specifically expanded in land plants,is important for polar growth and plant development. The Plant Journal, 48 (1):54-72.
[29]	Tenhaken R, Doerks T, Bork P. 2005. DCD-a novel plant specific domain in proteins involved in development and programmed cell death. BMC Bioinformatics,6:169.
[30]	Tillett R L, Wheatley M D, Tattersall E A, Schlauch K A, Cramer G R, Cushman J C. 2012. The Vitis vinifera C-repeat binding protein 4(VvCBF4) transcriptional factor enhances freezing tolerance in wine grape. Plant Biotechnology Journal, 10 (1):105-124.
[31]	Uemura T, Kim H, Saito C, Ebine K, Ueda T, Schulze-Lefert P, Nakano A. 2012. Qa-SNAREs localized to the trans-Golgi network regulate multiple transport pathways and extracellular disease resistance in plants. Proceedings of the National Academy of Sciences of the United States of America, 109 (5):1784-1789.
[32]	Wang L, Yao W, Zhang X, Tang Y J, Van Nocker S, Wang Y J, Zhang C H. 2023. The putative ABCG transporter VviABCG20 from grapevine (Vitis vinifera)is strongly expressed in the seed coat of developing seeds and may participate in suberin biosynthesis. Physiology and Molecular Biology of Plants, 29 (1):23-34.
[33]	Wang X, Liu Z J, Zhang F, Xiao H, Cao S, Xue H, Liu W W, Su Y, Liu Z Y, Zhong H X, Zhang F C, Ahmad B, Long Q M, Zhang Y C, Liu Y T, Gan Y, Hou T, Jin Z X, Wu X Y, Liu G T, Wang Y W, Peng Y L, Zhou Y F. 2024. Integrative genomics reveals the polygenic basis of seedlessness in grapevine. Current Biology,34:3763-3777.
[34]	Yao J, Zhang S L, Wu N, Li X M, Ahmad B, Wu J Y, Guo R R, Wang X P. 2023. KNOX transcription factor VvHB63 affects grape seed development by interacting with protein VvHB06. Plant Science,330:111665.
[35]	Zhang Hecui, Liu Jing, Lian Xiaoping, Zeng Jing, Yang Kun, Zhang Xuejie, Yang Dan, Shi Songmei, Gao Qiguo, Zhu Liquan. 2016. Expression and interaction between ROH1 and EXO70A1 in reproductive development. Scientia Agricultura Sinica, 49 (4):775-783. (in Chinese)
	张贺翠, 柳菁, 廉小平, 曾静, 杨昆, 张学杰, 杨丹, 施松梅, 高启国, 朱利泉. 2016. 甘蓝ROH1与EXO70A1的表达与相互作用. 中国农业科学, 49 (4):775-783.
[36]	Zhang Songlin. 2023. Functional analysis of grape transcription factor VvMADS39 and VvMADS28 and its regulation mechanism on ovule development[Ph. D. Dissertation]. Yangling: Northwest A & F University. (in Chinese)
	张松霖. 2023. 葡萄转录因子VvMADS39和VvMADS28功能分析及其对胚珠发育的调控研究[博士论文]. 杨凌: 西北农林科技大学.
[37]	Zhang Y, Immink R, Liu C M, Emons A M, Ketelaar T. 2013. The Arabidopsis exocyst subunit sec3A is essential for embryo development and accumulates in transient puncta at the plasma membrane. The New Phytologist, 199 (1):74-88.
[38]	Zhang Y, Wang Y M, Liu R T, Fei Z J, Fan X C, Jiang J F, Sun L, Meng X, Liu C H. 2024. Antibody array-based proteome approach reveals proteins involved in grape seed development. Plant Physiology, 195 (1):462-478.
[39]	Zhang Yucheng, Niu Jiao, Xu Xiaozhao, Wang Hua, Wang Xiping. 2012. Evolutionary,expression and promoter analysis of grape aldehyde dehydrogenase(ALDH2)genes. Journal of Northwest A & F University(Natural Science Edition), 40 (9):169-180. (in Chinese)
	张玉成, 牛姣, 徐晓召, 王华, 王西平. 2012. 葡萄醛脱氢酶(ALDH2)基因进化、表达及启动子功能的生物信息学分析. 西北科技大学学报(自然科学版), 40 (9):169-180.
[40]	Zhu Peipei, Qin Haoxiang, Zhang Jianxia. 2023. Changes of endogenous hormones and polyamines during ovule development of stenospermocarpic seedless grape. Scientia Agricultura Sinica, 56 (23):4789-4800. (in Chinese)
	朱佩佩, 秦浩翔, 张剑侠. 2023. 无核葡萄胚珠发育过程中内源激素及多胺含量的变化. 中国农业科学, 56 (23):4789-4800.

用途 Purpose	引物名称 Primer name	上游序列（5′-3′） Forward sequence	下游序列（5′-3′） Reverse sequence
转录自激活验证 Transcriptional self-activation validation	VvDDF2-BD	atggccatggaggccgaattcATGGACTTCTTCAATCAATTTTGTAATT	cgctgcaggtcgacggatccTCAAATTGAGTAACTCCATAGTGACATATC
转录自激活验证 Transcriptional self-activation validation	VvDDF2-D1-BD	atgg ccatggaggccgaattcATGGACTTCTTCAATCAATTTTGTAATT	cgctgcaggtcgacggatccCTGAGGCCGGAACGCCTC
酵母双杂交点对点回复验证 Yeast two-hybrid point-to-point response validation	VvEXO70A1-AD	gccatggaggccagtgaattcATGGGCGTAGCAGTAGGCATG	cagctcgagctcgatggatccTCGCTTGGCTTCATTCAAGGTT
	VvBBP-AD	gccatggaggccagtgaattcATGGCTGTGGGAAGAGGCAGTG	cagctcgagctcgatggatccAGTAGCAGTTACTGCTATCTTCATT
BiFC实验 BiFC assay	VvDDF2-NE	ggcgcgccactagtggatccATGGACTTCTTCAATCAATTTTGTAATT	catcccgggagcggtaccAATTGAGTAACTCCATAGTGACATATC
BiFC实验 BiFC assay	VvEXO70A1-CE	ggcgcgccactagtggatccATGGGCGTAGCAGTAGGCATG	catcccgggagcggtaccTCGCTTGGCTTCATTCAAGGTT
Split-Luc实验 Split-Luc assay	VvDDF2-N-Luc	tcggtacccgggatccATGGACTTCTTCAATCAATTTTGTAATT	acgagatctggtcgacAATTGAGTAACTCCATAGTGACATATC
Split-Luc实验 Split-Luc assay	VvEXO70A1-C-Luc	acgcgtcccggggcggtaccATGGGCGTAGCAGTAGGCATG	ta cgaacgaaagctctgcagTTATCGCTTGGCTTCATTCAAGGTT
基因表达分析 Gene expression analysis	VvActin-qPCR	GATTCTGGTGATGGTGTGAGT	GACAATTTCCCGTTCAGCAGT
基因表达分析 Gene expression analysis	VvEXO70A1-qPCR	CGTCAGGCAGAGGGTAAAG	AGGAGGGTCGCAGTGAGTT

用途 Purpose	引物名称 Primer name	上游序列（5′-3′） Forward sequence	下游序列（5′-3′） Reverse sequence
转录自激活验证 Transcriptional self-activation validation	VvDDF2-BD	atggccatggaggccgaattcATGGACTTCTTCAATCAATTTTGTAATT	cgctgcaggtcgacggatccTCAAATTGAGTAACTCCATAGTGACATATC
转录自激活验证 Transcriptional self-activation validation	VvDDF2-D1-BD	atgg ccatggaggccgaattcATGGACTTCTTCAATCAATTTTGTAATT	cgctgcaggtcgacggatccCTGAGGCCGGAACGCCTC
酵母双杂交点对点回复验证 Yeast two-hybrid point-to-point response validation	VvEXO70A1-AD	gccatggaggccagtgaattcATGGGCGTAGCAGTAGGCATG	cagctcgagctcgatggatccTCGCTTGGCTTCATTCAAGGTT
	VvBBP-AD	gccatggaggccagtgaattcATGGCTGTGGGAAGAGGCAGTG	cagctcgagctcgatggatccAGTAGCAGTTACTGCTATCTTCATT
BiFC实验 BiFC assay	VvDDF2-NE	ggcgcgccactagtggatccATGGACTTCTTCAATCAATTTTGTAATT	catcccgggagcggtaccAATTGAGTAACTCCATAGTGACATATC
BiFC实验 BiFC assay	VvEXO70A1-CE	ggcgcgccactagtggatccATGGGCGTAGCAGTAGGCATG	catcccgggagcggtaccTCGCTTGGCTTCATTCAAGGTT
Split-Luc实验 Split-Luc assay	VvDDF2-N-Luc	tcggtacccgggatccATGGACTTCTTCAATCAATTTTGTAATT	acgagatctggtcgacAATTGAGTAACTCCATAGTGACATATC
Split-Luc实验 Split-Luc assay	VvEXO70A1-C-Luc	acgcgtcccggggcggtaccATGGGCGTAGCAGTAGGCATG	ta cgaacgaaagctctgcagTTATCGCTTGGCTTCATTCAAGGTT
基因表达分析 Gene expression analysis	VvActin-qPCR	GATTCTGGTGATGGTGTGAGT	GACAATTTCCCGTTCAGCAGT
基因表达分析 Gene expression analysis	VvEXO70A1-qPCR	CGTCAGGCAGAGGGTAAAG	AGGAGGGTCGCAGTGAGTT

编号 Number	登录号 Sequence ID	蛋白名称 Protein name	功能注释 Annotation	参考文献 Reference
1	XM_010652827.3	腺苷酸激酶同工酶6同源物 Adenylate kinase isoenzyme 6 homolog [Vitis vinifera（wine grape）]	腺苷酸激酶（ADK）是一种能量代谢的关键酶，维持细胞能量动态平衡，在植物中参与调节生物产量和逆境应答等过程 Adenylate kinase（ADK）is a key enzyme in energy metabolism that maintains the dynamic balance of cellular energy，and is involved in regulating biological production and response to adversity in plants	Gong et al.,2010
2	XM_010656680.3	反式高尔基体网状结构定位的SYP41相互作用蛋白1 trans-Golgi network- localized SYP41-interacting protein 1[V. vinifera（wine grape）]	拟南芥SYP4家族定位于TGN（trans-Golgi Network），参与种子贮藏蛋白前体运输途径，调控植物生长发育 The SYP4 family in Arabidopsis is localized in the TGN （trans-Golgi Network）and is involved in the seed storage protein precursor transport pathway，regulating plant growth and development	Uemura et al.,2012
3	XM_003631207.4	2-亚甲基-呋喃-3-酮还原酶 2-Methylene-furan-3-one reductase [V. vinifera（wine grape）]	一种乙醇脱氢酶（ADH），与果实成熟过程中醇的形成密切相关，GmADH1可能参与了大豆质核互作雄性不育（CMS）的育性调控 A type of alcohol dehydrogenase（ADH），closely related to the formation of alcohols during fruit ripening，GmADH1 may be involved in the regulation of cytoplasmic-nuclear male sterility（CMS）in soybean	丁先龙,2017
续表2
编号 Number	登录号 Sequence ID	蛋白名称 Protein name	功能注释 Annotation	参考文献 Reference
4	XM_002266352.3	DCD结构域蛋白NRP-B DCD domain-containing protein NRP-B [V. vinifera（wine grape）]	DCD/NRP蛋白在植物激素的响应、胚胎发育和病原体或臭氧导致的程序性细胞死亡中起作用 DCD/NRP proteins play a role in plant hormone response，embryonic development and programmed cell death caused by pathogens or ozone	Tenhaken et al.,2005
5	XM_002285094.5	4-羟基-3-甲基丁-2-烯基二磷酸还原酶（HDR） 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase （ferredoxin），chloroplastic [V. vinifera（wine grape）]	与质体发育有关，定位于叶绿体，参与植物异戊烯基二磷酸（IPP）和胆固醇生物合成，是萜烯类物质生物合成的重要限速酶 Associated with plastid development，localized in chloroplasts，involved in the biosynthesis of isopentenyl diphosphate（IPP）and cholesterol in plants，important rate limiting enzyme for terpenoid biosynthesis	陈天池等,2023
6	XM_002275951.5	碱性蓝铜蛋白 Basic blue protein[V. vinifera （wine grape）]	一种植物铜氧还蛋白，属于蓝铜蛋白家族的成员，参与生殖授粉、器官形成和胁迫响应 A plant copper oxyreticulin protein，a member of the blue copper protein family，involved in reproductive pollination，organ formation and stress response	Dong et al.,2005
7	XM_002268074.5	胞外囊复合体亚基 Exocyst complex component EXO70A1[V. vinifera（wine grape）]	参与胞质分裂、细胞极性生长和形态建成、花粉萌发和花粉管的极性伸长、种皮细胞壁的形成和PIN蛋白的再循环和极性定位、植物免疫反应和自噬等过程 Involved in cytoplasmic division，polar growth and morphogenesis of cells，pollen germination and polar elongation of pollen tubes，formation of seed coat cell wall，recirculation and polar localization of PIN proteins，plant immune response and autophagy	Hala et al.,2008
8	XM_002285852.4	BTB/POZ结构域蛋白 BTB/POZ domain-containing protein At4g08455[V. vinifera （wine grape）]	与植物激素信号转导、抗逆性、蛋白质泛素化、细胞骨架构成、次生代谢产物合成等有关 Related to plant hormone signal transduction，stress resistance，protein ubiquitination，cytoskeleton composition and secondary metabolite synthesis	Julian et al.,2019
9	XM_059743404.1	胺氧化酶 Amine oxidase [copper- containing] gamma 1[V. vinifera （wine grape）]	参与植物多胺合成代谢通路 Involved in plant polyamine synthesis metabolic pathway	Fortes et al.,2019
10	XM_010653238.3	羟甲基戊二酰辅酶A裂解酶 Hydroxymethylglutaryl-CoA lyase，mitochondrial[V. vinifera（wine grape）]	一种线粒体蛋白，在大豆中调节豆荚色素沉着和开裂 A mitochondrial proteins that regulates pod pigmentation and cracking in soybean	Lyu et al.,2023
11	XM_010651472.2	脂肪酶 Lipase [V. vinifera（wine grape）]	参与植物种子中的油脂代谢过程，影响种子发育；拟南芥GDSL脂肪酶基因在胚乳中表达并且调节胚胎的发育 Involvement in lipid metabolism in plant seeds that affects seed development. The GDSL lipase gene in Arabidopsis is expressed in endosperm and regulates embryo development	Clauss et al.,2011
12	XM_002283173.4	谷胱甘肽转移酶（GST） Glutathione S-transferase F9[V. vinifera（wine grape）]	植物体中广泛存在的一种多功能酶，在植物的生长发育、次生代谢和抵御非生物胁迫发挥作用 A multifunctional enzyme widely found in plants that plays a role in plant growth and development，secondary metabolism and resistance to abiotic stresses	Rezaei et al.,2013
13	XM_059740061.1	核糖体大亚基蛋白 Large ribosomal subunit protein bL21m[V. vinifera（wine grape）]	参与蛋白质合成，调控油菜自交不亲和反应 Involvement in protein synthesis and regulation of self-incompatibility response in oilseed rape	Khan,2020
14	XM_010657812.3	醛脱氢酶7家族成员 Aldehyde dehydrogenase family 7 member [V. vinifera（wine grape）]	醛脱氢酶（ALDH）参与植物对生物及非生物的胁迫反应以及对生长发育的调节 Aldehyde dehydrogenase（ALDH）is involved in plant responses to biotic and abiotic stresses and regulation of growth and development	张玉成等,2012

编号 Number	登录号 Sequence ID	蛋白名称 Protein name	功能注释 Annotation	参考文献 Reference
1	XM_010652827.3	腺苷酸激酶同工酶6同源物 Adenylate kinase isoenzyme 6 homolog [Vitis vinifera（wine grape）]	腺苷酸激酶（ADK）是一种能量代谢的关键酶，维持细胞能量动态平衡，在植物中参与调节生物产量和逆境应答等过程 Adenylate kinase（ADK）is a key enzyme in energy metabolism that maintains the dynamic balance of cellular energy，and is involved in regulating biological production and response to adversity in plants	Gong et al.,2010
2	XM_010656680.3	反式高尔基体网状结构定位的SYP41相互作用蛋白1 trans-Golgi network- localized SYP41-interacting protein 1[V. vinifera（wine grape）]	拟南芥SYP4家族定位于TGN（trans-Golgi Network），参与种子贮藏蛋白前体运输途径，调控植物生长发育 The SYP4 family in Arabidopsis is localized in the TGN （trans-Golgi Network）and is involved in the seed storage protein precursor transport pathway，regulating plant growth and development	Uemura et al.,2012
3	XM_003631207.4	2-亚甲基-呋喃-3-酮还原酶 2-Methylene-furan-3-one reductase [V. vinifera（wine grape）]	一种乙醇脱氢酶（ADH），与果实成熟过程中醇的形成密切相关，GmADH1可能参与了大豆质核互作雄性不育（CMS）的育性调控 A type of alcohol dehydrogenase（ADH），closely related to the formation of alcohols during fruit ripening，GmADH1 may be involved in the regulation of cytoplasmic-nuclear male sterility（CMS）in soybean	丁先龙,2017
续表2
编号 Number	登录号 Sequence ID	蛋白名称 Protein name	功能注释 Annotation	参考文献 Reference
4	XM_002266352.3	DCD结构域蛋白NRP-B DCD domain-containing protein NRP-B [V. vinifera（wine grape）]	DCD/NRP蛋白在植物激素的响应、胚胎发育和病原体或臭氧导致的程序性细胞死亡中起作用 DCD/NRP proteins play a role in plant hormone response，embryonic development and programmed cell death caused by pathogens or ozone	Tenhaken et al.,2005
5	XM_002285094.5	4-羟基-3-甲基丁-2-烯基二磷酸还原酶（HDR） 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase （ferredoxin），chloroplastic [V. vinifera（wine grape）]	与质体发育有关，定位于叶绿体，参与植物异戊烯基二磷酸（IPP）和胆固醇生物合成，是萜烯类物质生物合成的重要限速酶 Associated with plastid development，localized in chloroplasts，involved in the biosynthesis of isopentenyl diphosphate（IPP）and cholesterol in plants，important rate limiting enzyme for terpenoid biosynthesis	陈天池等,2023
6	XM_002275951.5	碱性蓝铜蛋白 Basic blue protein[V. vinifera （wine grape）]	一种植物铜氧还蛋白，属于蓝铜蛋白家族的成员，参与生殖授粉、器官形成和胁迫响应 A plant copper oxyreticulin protein，a member of the blue copper protein family，involved in reproductive pollination，organ formation and stress response	Dong et al.,2005
7	XM_002268074.5	胞外囊复合体亚基 Exocyst complex component EXO70A1[V. vinifera（wine grape）]	参与胞质分裂、细胞极性生长和形态建成、花粉萌发和花粉管的极性伸长、种皮细胞壁的形成和PIN蛋白的再循环和极性定位、植物免疫反应和自噬等过程 Involved in cytoplasmic division，polar growth and morphogenesis of cells，pollen germination and polar elongation of pollen tubes，formation of seed coat cell wall，recirculation and polar localization of PIN proteins，plant immune response and autophagy	Hala et al.,2008
8	XM_002285852.4	BTB/POZ结构域蛋白 BTB/POZ domain-containing protein At4g08455[V. vinifera （wine grape）]	与植物激素信号转导、抗逆性、蛋白质泛素化、细胞骨架构成、次生代谢产物合成等有关 Related to plant hormone signal transduction，stress resistance，protein ubiquitination，cytoskeleton composition and secondary metabolite synthesis	Julian et al.,2019
9	XM_059743404.1	胺氧化酶 Amine oxidase [copper- containing] gamma 1[V. vinifera （wine grape）]	参与植物多胺合成代谢通路 Involved in plant polyamine synthesis metabolic pathway	Fortes et al.,2019
10	XM_010653238.3	羟甲基戊二酰辅酶A裂解酶 Hydroxymethylglutaryl-CoA lyase，mitochondrial[V. vinifera（wine grape）]	一种线粒体蛋白，在大豆中调节豆荚色素沉着和开裂 A mitochondrial proteins that regulates pod pigmentation and cracking in soybean	Lyu et al.,2023
11	XM_010651472.2	脂肪酶 Lipase [V. vinifera（wine grape）]	参与植物种子中的油脂代谢过程，影响种子发育；拟南芥GDSL脂肪酶基因在胚乳中表达并且调节胚胎的发育 Involvement in lipid metabolism in plant seeds that affects seed development. The GDSL lipase gene in Arabidopsis is expressed in endosperm and regulates embryo development	Clauss et al.,2011
12	XM_002283173.4	谷胱甘肽转移酶（GST） Glutathione S-transferase F9[V. vinifera（wine grape）]	植物体中广泛存在的一种多功能酶，在植物的生长发育、次生代谢和抵御非生物胁迫发挥作用 A multifunctional enzyme widely found in plants that plays a role in plant growth and development，secondary metabolism and resistance to abiotic stresses	Rezaei et al.,2013
13	XM_059740061.1	核糖体大亚基蛋白 Large ribosomal subunit protein bL21m[V. vinifera（wine grape）]	参与蛋白质合成，调控油菜自交不亲和反应 Involvement in protein synthesis and regulation of self-incompatibility response in oilseed rape	Khan,2020
14	XM_010657812.3	醛脱氢酶7家族成员 Aldehyde dehydrogenase family 7 member [V. vinifera（wine grape）]	醛脱氢酶（ALDH）参与植物对生物及非生物的胁迫反应以及对生长发育的调节 Aldehyde dehydrogenase（ALDH）is involved in plant responses to biotic and abiotic stresses and regulation of growth and development	张玉成等,2012

Screening and Validation of Interacting Proteins of the Grape Ovule Development Transcription Factor VvDDF2

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 8

References 40

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	GENG Hao, GE Mengqing, DING Yunlong, XU Weidong, CONG Chunlei, YAN Hongwei, QIAN Yu, LIU Yufeng. A New Medium Ripening Table Grape Cultivar‘Yuanjinxiang’ [J]. Acta Horticulturae Sinica, 2025, 52(S2): 31-32.
[2]	GAO Min, ZHAO Ning, LI Zhi, WANG Xianhang, TU Mingxing, GUO Junqiang, WANG Xiping. A New Table Grape Cultivar‘Qinpu 3 Hao’ [J]. Acta Horticulturae Sinica, 2025, 52(S2): 33-34.
[3]	WEI Xixi, ZHAO Ning, LI Zhi, WANG Xianhang, TU Mingxing, GAO Min, WANG Xiping. A New Table Grape Cultivar‘Qinpu 4 Hao’ [J]. Acta Horticulturae Sinica, 2025, 52(S2): 35-36.
[4]	HAO Yan, ZHU Yanfang, GAO Bo. A New Seedless Table Grape Cultivar‘Ziyan’ [J]. Acta Horticulturae Sinica, 2025, 52(S2): 37-38.
[5]	NI Peijin, AI Jun, SHI Guangli, WANG Zhenxing, SUN Dan, SUN Yanfeng, LIU Xiaoying. A New Cold-Resistant Wine Grape Cultivar‘Fuhong’ [J]. Acta Horticulturae Sinica, 2025, 52(S2): 39-40.
[6]	SHU Nan, LU Wenpeng, FAN Shutian, WANG Yanli, LIU Guoliang, LI Changyu, SUN Bowei, LIU Xinhua, WANG Yue, TAN Yue, ZHANG Baoxiang, TIAN Taiping. A New Wine Grape Cultivar‘Zijing Xianglu’ [J]. Acta Horticulturae Sinica, 2025, 52(S2): 43-44.
[7]	HAN Jiayu, GUO Rongrong, ZHANG Ying, SHI Xiaofang, CAO Xiongjun, HUANG Guiyuan, BAI xianjin, XIE Taili, YU Huan, XIE Shuyu, LIN Ling. Low Chilling Requirement Grape New Cultivar‘Guipu 9’ [J]. Acta Horticulturae Sinica, 2025, 52(S1): 45-46.
[8]	SHU Nan, LU Wenpeng, YANG Yiming, XU Peilei, LI Jiaqi, LIU Tao, QU Bingzhang. A New Wine Grape Cultivar‘Zijinglu’ [J]. Acta Horticulturae Sinica, 2025, 52(S1): 47-48.
[9]	ZHU Cunlan, FAN Junliang, WANG Kaitong, WEI Meng, YANG Liang, LIU Haotian, SI Huaijun, ZHANG Ning. Function Analysis ofStRGLG1in Regulating Potato to Drought Stress Response [J]. Acta Horticulturae Sinica, 2025, 52(9): 2329-2342.
[10]	REN Tingting, ZHANG Tingxiu, ZHAN Liang, ZHANG Zhichang, REN Yiran, JI Xinglong, WANG Peipei, LIU Yuanxia, MENG Qingfu, LIU Gengsen, FANG Jinggui, LENG Xiangpeng. Effect of Different Light Quality on Berry Quality of‘Shine Muscat’Grape [J]. Acta Horticulturae Sinica, 2025, 52(9): 2464-2476.
[11]	LIANG Jing, ZENG Baozhen, LIANG Guoping, MAO Juan, CHEN Baihong. Grapevine VvARF18 Regulates Fruit Expansion and Screening of its Interacting Proteins [J]. Acta Horticulturae Sinica, 2025, 52(4): 821-834.
[12]	WANG Yi, LIANG Zhenchang. Analysis of Genetic Mechanism of Grape Distant Hybridization Based on Population Transcriptome [J]. Acta Horticulturae Sinica, 2025, 52(4): 835-845.
[13]	WANG Rui, WU Hongfei, ZHANG Changyuan, CAO Haishun, TAN Delong, GUO Jinju, WANG Yunlong, WANG Rufang, YUAN Yu, WU Tingquan. CsPUB54 Negatively Regulates Cucumber Resistance to Phytophthora Melonis by Interacting with PmRXLR1 Effector [J]. Acta Horticulturae Sinica, 2025, 52(3): 591-602.
[14]	XING Zhigan, LEI Xiangzhao, WANG Haochen, FENG Mingxin, LI Jingwen, LIU Yujia, FANG Yulin, MENG Jiangfei. Physiological Response of Shine Muscat Grape Seedlings Grafted with Different Rootstocks to Combined Stress of Salt and Low Temperature [J]. Acta Horticulturae Sinica, 2025, 52(3): 693-704.
[15]	ZHAO Yulei, LI Shan, LI Chengnan, MA Jinlong, MA Hongyi, YIN Xiao. Proteomics Studies on the Early Stages of Botrytis cinerea Infection in Grapevine Leaves [J]. Acta Horticulturae Sinica, 2025, 52(2): 279-291.