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Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (8): 1927-1948.doi: 10.16420/j.issn.0513-353x.2023-0894

• Reviews • Previous Articles     Next Articles

Advances in the CRISPR/Cas DNA-Free Genome Editing in Plant

LU Di1,2, HU Chunhua1, SHENG Ou1, YANG Qiaosong1, DOU Tongxin1, HE Weidi1, DENG Guiming1, GAO Huijun1, LIU Siwen1, LI Chunyu1, DONG Tao1, YI Ganjun1,*(), BI Fangcheng1,*()   

  1. 1 Institute of Fruit Tree Research,Guangdong Academy of Agricultural Science/Key Laboratory of South Subtropical Fruit Tree Biology and Genetic Resources Utilization,Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Research,Guangzhou 510640,China
    2 College of Horticulture and Forestry,Huazhong Agricultural University,Wuhan 430070,China
  • Received:2024-01-26 Revised:2024-05-29 Online:2024-08-25 Published:2024-08-22
  • Contact: YI Ganjun, BI Fangcheng

Abstract:

CRISPR/Cas genome editing technology has become an important tool for the genetic improvement of crops. However,common genome editing methods of Agrobacterium-mediated or particle bombardment delivery DNA can lead to the integration of exogenous vector fragments into the plant genome,raising consumer concerns about its potential biosafety. At present,some plants have established DNA-free genome editing systems,but there are problems such as low delivery efficiency,low editing efficiency,and regeneration difficulties. In this review,the advances in the establishment and application of plant CRISPR/Cas DNA-free genome editing system were summaried from three aspects:the vector elements,delivery methods and the commonly used editing strategy. The main existing problems and future perspectives were discussed.

Key words: CRISPR/Cas, genome editing, DNA-free editing strategy, vector elements, delivery methods, biosafety