Abstract:

In order to explore the effects of biological-organic fertilizer combined with specialized fertilizer on soil nutrients，soil microbial diversity and yield in vineyards，this study selected the‘Shine Muscat’grape under protected cultivation as the research object，and conventional fertilization treatment as the control treatment. The research results showed that the soil pH，available phosphorus，available potassium，and exchangeable calcium or magnesium contents under the biological-organic fertilizer combined with specialized fertilizer and specialized water-soluble fertilizer treatment were significantly increased（P < 0.05）compared with the control treatment. The value of soil bacterial diversity Chao1 index，Shannon index，and total OUT on showed a significant increase，but the soil fungal diversity Chao1 index and Shannon index had no significant changes. The results of Illumina MiSeq high-throughput sequencing analysis showed that soil bacterial communities can be classified into 40 phyla，108 classes，240 orders，326 families，and 595 genera，while soil fungal communities can be classified into 13 phyla，32 classes，71 orders，126 families，and 172 genera. Compared with control treatment，biological-organic fertilizer combined with specialized fertilizer and specialized water-soluble fertilizer treatments at the bacterial phyla level，the relative abundance of Actinobacteria increased by 80.33%，and the bacterial classes level，the relative abundance of α-Proteobacteria and Actinobacteria increased by 29.62% and 108.56%，respectively. At the fungi phyla level，the relative abundance of Ascomycota increased by 4.48%，and at the fungi classes level，Sordariomycetes increased by 10.42%，but Eurotiomycetes and Dothideomycetes decreased by 26.38% and 29.42%. Redundancy analysis（RDA）found that at the phyla level，the explanation rates of environmental factors for soil bacterial and fungal communities change were 64.21% and 56.91% respectively. Soil pH，available potassium，and exchangeable calcium or magnesium had a significant impact on the structure of soil microbial communities in vineyards. Compared to the control treatment，the yield and soluble solid content increased by 25.91% and 5.61% under the biological-organic fertilizer combined with specialized fertilizer and specialized water-soluble fertilizer treatment，respectively.

Key words: biological-organic fertilizers, fertilization patterns, vineyards, nutrients, microbial diversity