Effect of Different Pre-treated Temperatures on the Microspore Embryogenesis in Broccoli

doi:10.16420/j.issn.0513-353x.2014-0469

ACTA HORTICULTURAE SINICA ›› 2015, Vol. 42 ›› Issue (3): 563-568.doi: 10.16420/j.issn.0513-353x.2014-0469

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Effect of Different Pre-treated Temperatures on the Microspore Embryogenesis in Broccoli

SUN Ji-feng，FANG Zhi-yuan，YUAN Su-xia，LI Zhan-sheng，YANG Li-mei，ZHUANG Mu，ZHANG Yang-yong，SUN Pei-tian，and LIU Yu-mei*

Institute of Vegetable and Flowers of Chinese Academy of Agricultural Sciences，Beijing 100081，China

Online:2015-03-25 Published:2015-03-25

Abstract

Abstract: B908 and B1092 of broccoli（Brassica oleracea L. var. italica Plenck）were used to be the materials in the research on microspore embryogenesis. The nuclear division patterns and embryo development efficiency of isolated microspores of broccoli pretreated at different temperatures were investigated under a fluorescence microscopy by using DAPI staining. Four pretreated temperatures were 25 ℃，4 ℃，32.5 ℃，or 4 ℃→32.5 ℃（combined treatment）. The results showed that the nuclear division patterns were much the same under different temperature pretreatment. The B pathway started from symmetrical division that was the main pathway. The cotyledons-shaped embryo was formed after different developmental stages，microspore divided equally，multicellular structure packaged by the exine，original embryoid，globular embryoid，heart-shaped embryoid，torpedo-shaped embryiod，cotyledons- shaped embryoid. The embryoid development efficiency of isolated microspores of broccoli pretreated at different temperatures was different. The combined treatment（4 ℃→32.5 ℃）induced the highest frequency of microspore derived embryo.

Key words: broccoli, isolated microspore culture, pathway, embryogenesis yield

CLC Number:

S 635

SUN Ji-feng，FANG Zhi-yuan，YUAN Su-xia，LI Zhan-sheng，YANG Li-mei，ZHUANG Mu，ZHANG Yang-yong，SUN Pei-tian，and LIU Yu-mei*. Effect of Different Pre-treated Temperatures on the Microspore Embryogenesis in Broccoli[J]. ACTA HORTICULTURAE SINICA, 2015, 42(3): 563-568.

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Effect of Different Pre-treated Temperatures on the Microspore Embryogenesis in Broccoli

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