Abstract: The effect of methionine-selenium（Met-Se）on selenium（Se）content，physiological characteristics，and quality of the muskmelon（Cucumis melo‘M135’）were studied by spraying Met-Se solution on the leaves. Spraying the same amount of water was set as control in the spraying concentration test，Met-Se solution with different Se concentration（15，30 or 60 mg ? L-1）were respectively sprayed on the leaves of the muskmelon. Results indicated that the Se concentration of 30 mg ? L-1 Met-Se solution significantly increased the quality of muskmelon fruit compared with the control，and up to the selenium-rich standards. Taking none spraying as control in the spraying times test，sprayed Met-Se solution with Se concentration of 30 mg ? L-1 respectively once，twice or three times on the leaves. Results indicated thatsprayed Met-Se solution for 1–3 times led to an obvious increase in pigment content，photosynthetic rate（Pn），growth volume and yield. The contents of organic and inorganic Se，soluble sugars，proteins and free anomic acids in fruit were also higher than those of the control. The contents of Se and organic Se in muskmelon fruit treated with Met-Se solution for 2 times were 43.13 μg ? kg-1 and 35.38 μg ? kg-1 respectively，and the soluble sugar，protein and free anomic acid contents increased by 9.14%，31.72% and 33.90% respectively compared with those of the control. These data suggested that foliar spraying Met-Se not only increased the Se content in muskmelon fruits，but also enhanced the photosynthetic capacity，and improved the yield and integrated quality，especially sprayed Met-Se solution with Se concentration 30 mg ? L-1 for 2 times.

Key words: muskmelon, methionine-selenium, physiological characteristic, selenium content, quality