Construction of One Mutant Library and Research on Phenotypic Variation of M2 Population Leaves in Chinese Cabbage

ACTA HORTICULTURAE SINICA ›› 2014, Vol. 41 ›› Issue (8): 1609-1619.

• Vegetables • Previous Articles Next Articles

Construction of One Mutant Library and Research on Phenotypic Variation of M2 Population Leaves in Chinese Cabbage

LU Yin，LIU Meng-yang，ZHAO Jian-jun，WANG Yan-hua，LUO Shuang-xia，XUAN Shu-xin，DAI Shuang-yan，WANG Chao-shuo，and SHEN Shu-xing*

（Key Laboratory for Vegetable Germplasm Enhancement and Utilization of Hebei，College of Horticulture，Agricultural University of Hebei，Baoding，Heibei 071001，China）

Received:2014-04-29 Online:2014-08-25 Published:2014-08-25

Abstract

Abstract: Seeds of the Chinese cabbage inbred line‘A03’were treated by EMS with different concentrations in this study. The suitable EMS concentration for seed mutagenesis was determined as 0.4% by studying the germination rate，survival rate，fertility of M1 plants and survival rate of M2 individuals. By three ways of one time seeds treatment with EMS，two times of seeds treatment with EMS，and buds treatment with EMS combined with microspore culture，the Chinese cabbage mutant library contained 4 253 M1 families and their selfing seeds of M2 was constructed. For M2 individuals in the mutant library，leaf phenotypic traits of 6 404 seedlings at seedling stage and 7 756 plants at rosette stage were investigated，resulting in total mutant frequency of 37.62% and 26.33%，respectively.

Key words: Brassica rapa, EMS, mutant library, mutagenesis

CLC Number:

S 634.1

LU Yin，LIU Meng-yang，ZHAO Jian-jun，WANG Yan-hua，LUO Shuang-xia，XUAN Shu-xin，DAI Shuang-yan，WANG Chao-shuo，and SHEN Shu-xing*. Construction of One Mutant Library and Research on Phenotypic Variation of M2 Population Leaves in Chinese Cabbage[J]. ACTA HORTICULTURAE SINICA, 2014, 41(8): 1609-1619.

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Construction of One Mutant Library and Research on Phenotypic Variation of M2 Population Leaves in Chinese Cabbage

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