Abstract: Karyological study of 12 wild populations of Lilium sargentiae Wilson from Yunnan
Province was carried out by traditional squashing and pressing method. The results were the following.
The base number of L. sargentiae was 12（x = 12）. All of the 12 wild populations were diploid（2n = 2x =
24）with a karyotype of 3A and without B chromosome. Their first and second pairs of chromosomes were
either large median chromosomes（m）or large submedian chromosomes（sm）. All the first pairs of
chromosomes of the 12 populations had satellite chromosomes. The Karyotype asymmetry coefficient （As.K%）of the 12 populations were from 78.66% to 82.05%，which indicated the karyotype of L.
sargentiae was asymmetrical and advanced. The As.K% of 12 populations of L. sargentiae from high to
low was as follows：Maguan population > Shikan population > Longling population > Jiucheng population >
Shilin population = Luxi population > Shuanghe population > Xichou population = Shizong population >
Daguan population > Wenshan population > Zhaxi population，which might reflect the intraspecific
evolution relationships among populations in this species. The 12 populations of L. sargentiae could be
divided into 3 groups by cluster analysis when the threshold was 1.04. The above results indicated that
the chromosome number of L. sargentiae was stable，and the chromosome variation among populations
was generally coming from structure variation. The 12 populations had obvious differences in the
composition of chromosomes，the number and distribution of satellite chromosomes，arm ratio，
chromosome length ratio and As.K%. There were obvious karyological variations among populations of
L. sargentiae.

Key words: Lilium sargentiae, population, chromosome, karyotype