Rapid Method for the Extraction of Abscisic Acid from Strawberry and Analysis by Ultra-high Performance Liquid Chromatography

ACTA HORTICULTURAE SINICA ›› 2014, Vol. 41 ›› Issue (3): 577-584.

• Technologies and Methods • Previous Articles Next Articles

Rapid Method for the Extraction of Abscisic Acid from Strawberry and Analysis by Ultra-high Performance Liquid Chromatography

MA Hong-qi，CHEN Min-dong，ZHU Hai-sheng*，and WEN Qing-fang*

Crops Research Institute，Fujian Academy of Agricultural Sciences；Vegetable Research Center，Fujian Academy of
Agricultural Sciences；Fujian Engineering Research Center for Vegetables，Fuzhou 350013，China

Online:2014-03-25 Published:2014-03-25

Abstract

Abstract: This study was carried out to look for an optimized method for extraction of endogenous
abscisic acid（ABA）from strawberry and for ultra performance liquid chromatography（UPLC）analysis.
A rapid extracting method that using Methanol-ethyl acetate-formic acid（50︰50︰1，v/v）as extracting
liquid was found out，which can extract ABA from strawberry in half an hour. An efficient UPLC analysis
method for ABA was also established，Chromatographic separation was carried out by reversed Phase
ACQUITY UPLC BEH C18 column and with Methanol︰acetonitrile︰0.1% formic acid（20︰10︰70）
isocratic elution program，at column temperature of 30 ℃，injection volume of 10 μL，flowrate of
0.3 mL · min-1，and wavelength at 265 nm. Applying the extracting and UPLC analysis method，we
evaluated the content of ABA in strawberry fruit at different developments and ripening stages. The result
showed that with developing and ripening，content of ABA in strawberry was increasing，and reached the
highest level at the red ripening stage.

Key words: strawberry, ABA, UPLC, extraction

CLC Number:

S 668.4

MA Hong-Qi, CHEN Min-Dong, ZHU Hai-Sheng, WEN Qing-Fang. Rapid Method for the Extraction of Abscisic Acid from Strawberry and Analysis by Ultra-high Performance Liquid Chromatography[J]. ACTA HORTICULTURAE SINICA, 2014, 41(3): 577-584.

[1]	YUAN Xin, XU Yunhe, ZHANG Yupei, SHAN Nan, CHEN Chuying, WAN Chunpeng, KAI Wenbin, ZHAI Xiawan, CHEN Jinyin, GAN Zengyu. Studies on AcAREB1 Regulating the Expression of AcGH3.1 During Postharvest Ripening of Kiwifruit [J]. Acta Horticulturae Sinica, 2023, 50(1): 53-64.
[2]	HE Chengyong, ZHAO Xiaoli, XU Tengfei, GAO Dehang, LI Shifang, WANG Hongqing. Genome Sequence Analysis of Strawberry Virus 1 from Shandong Province，China [J]. Acta Horticulturae Sinica, 2023, 50(1): 153-160.
[3]	YANG Lei, LI Li, DONG Hui, FENG Jia, ZHANG Jianjun, FAN Jingfang, YANG Qiuye, and YANG Li, . A New Strawberry Cultivar‘Shimei 11’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 79-80.
[4]	ZHAO Xia, LI Gang, LIU Lifeng, SONG Yanhong, and ZHOU Houcheng. A New Strawberry Cultivar‘Huashuo 1’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 81-82.
[5]	SUN Simiao, WANG Kun, GAO Yuan, WANG Dajiang, and LI Lianwen. A New Ornamental Crabapple Cultivar‘Zichen’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 267-268.
[6]	DONG Jing, CHANG Linlin, WANG Guixia, ZHONG Chuanfei, WEI Yongqing, SUN Jian, SUN Rui, ZHANG Hongli, GAO Yongshun, XU Liping, TAO Pang, LUO Zhiwei, and ZHANG Yuntao, . A New Ever-bearing Strawberry Cultivar‘Jinghong’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 61-62.
[7]	ZHU Linlin, ZHOU Wanfei, NING Xuan, ZHU Jun, XI Wan, ZENG Xumei, XIONG Kangshun, WANG Caiyun, LUO Jing, ZHENG Riru. Analysis of Free Aromatic Components of Different Osmanthus fragrans Cultivars [J]. Acta Horticulturae Sinica, 2022, 49(11): 2395-2406.
[8]	XIE Siyi, ZHOU Chengzhe, ZHU Chen, ZHAN Dongmei, CHEN Lan, WU Zuchun, LAI Zhongxiong, GUO Yuqiong. Genome-wide Identification and Expression Analysis of CsTIFY Transcription Factor Family Under Abiotic Stress and Hormone Treatments in Camellia sinensis [J]. Acta Horticulturae Sinica, 2022, 49(1): 100-116.
[9]	A New Chaenomeles Crabapple Cultivar‘Caiyu’. A New Chaenomeles Crabapple Cultivar‘Caiyu’ [J]. Acta Horticulturae Sinica, 2021, 48(S2): 2975-2976.
[10]	ZHOU Jinchuan, GUAN Qide, GUAN Xueying, ZHAO Yuhan, ZHANG Haijuan, and WANG Xiangbo. A New Chaenomeles Crabapple Cultivar‘Zui Xishi’ [J]. Acta Horticulturae Sinica, 2021, 48(S2): 2977-2978.
[11]	ZHANG Zhifang, YUAN lei, CHENG Nini, LIU Zongzhao, and CHEN Zhiqun, . A New Ornamental Crabapple Cultivar‘Fengguanhong’ [J]. Acta Horticulturae Sinica, 2021, 48(S2): 2979-2980.
[12]	CHEN Li, ZHOU Jinchuan, HAN Qingdian, WANG Xiangbo, GUAN Qide, and CHEN Zhiqun, . A New Crabapple Cultivar‘Menghuijuan’ [J]. Acta Horticulturae Sinica, 2021, 48(S2): 2981-2982.
[13]	ZHANG Haijuan, GUAN Qide, ZHOU Jinchuan, and GAO Yueheng. A New Chaenomeles Crabapple Cultivar‘Huatiannü’ [J]. Acta Horticulturae Sinica, 2021, 48(S2): 2983-2984.
[14]	YANG Liyuan, WANG Qian, WANG Xuhui, XU Tongda, MA Jun. The Important Biological Function of T111,an Evolutional Conserved Amino Acid in FveTAA1,a Key Enzyme in Strawberry Auxin Biosynthesis [J]. Acta Horticulturae Sinica, 2021, 48(9): 1695-1705.
[15]	HONG Yanhong, YE Qinghua, LI Zekun, WANG Wei, XIE Qian, CHEN Qingxi, CHEN Jianqing. Accumulation of Anthocyanins in Red-flowered Strawberry‘Meihong’Petals and Expression Analysis of MYB Gene [J]. Acta Horticulturae Sinica, 2021, 48(8): 1470-1484.

Rapid Method for the Extraction of Abscisic Acid from Strawberry and Analysis by Ultra-high Performance Liquid Chromatography

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments